KEGG   ENZYME: 3.5.1.117Help
Entry
EC 3.5.1.117                Enzyme                                 

Name
6-aminohexanoate-oligomer endohydrolase;
endo-type 6-aminohexanoate oligomer hydrolase;
Ahx endo-type-oligomer hydrolase;
6-aminohexanoate oligomer hydrolase;
Ahx-oligomer hydrolase;
nylon hydrolase;
nylon-oligomer hydrolase;
NylC;
nylon-6 hydrolase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
6-aminohexanoate oligomer endoamidohydrolase
Reaction(IUBMB)
[N-(6-aminohexanoyl)]n + H2O = [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x [RN:R10976]
Reaction(KEGG)
R10976;
(other) R10978 R10979
Show
Substrate
[N-(6-aminohexanoyl)]n [CPD:C20988];
H2O [CPD:C00001]
Product
[N-(6-aminohexanoyl)]n-x;
[N-(6-aminohexanoyl)]x
Comment
The enzyme is involved in degradation of nylon-6 oligomers. It degrades linear or cyclic oligomers of poly(6-aminohexanoate) with a degree of polymerization greater than three (n > 3) by endo-type cleavage, to oligomers of a length of two or more (2 <= x < n). It shows negligible activity with N-(6-aminohexanoyl)-6-aminohexanoate (cf. EC 3.5.1.46, 6-aminohexanoate-oligomer exo hydrolase) or with 1,8-diazacyclotetradecane-2,9-dione (cf. EC 3.5.2.12, 6-aminohexanoate-cyclic-dimer hydrolase).
History
EC 3.5.1.117 created 2014
Pathway
Caprolactam degradation
Microbial metabolism in diverse environments
Orthology
K19963  
6-aminohexanoate-oligomer endohydrolase
Reference
1  [PMID:8285701]
  Authors
Kakudo S, Negoro S, Urabe I, Okada H
  Title
Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.
  Journal
Appl. Environ. Microbiol. 59 (1993) 3978-80.
  Sequence
[pg:6390782]
Reference
2  [PMID:17827307]
  Authors
Yasuhira K, Tanaka Y, Shibata H, Kawashima Y, Ohara A, Kato D, Takeo M, Negoro S
  Title
6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp. strain KY5R and Kocuria sp. strain KY2.
  Journal
Appl. Environ. Microbiol. 73 (2007) 7099-102.
Reference
3  [PMID:22187439]
  Authors
Negoro S, Shibata N, Tanaka Y, Yasuhira K, Shibata H, Hashimoto H, Lee YH, Oshima S, Santa R, Oshima S, Mochiji K, Goto Y, Ikegami T, Nagai K, Kato D, Takeo M, Higuchi Y
  Title
Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis.
  Journal
J. Biol. Chem. 287 (2012) 5079-90.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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