| Entry |
|
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| Name |
glutaryl-7-aminocephalosporanic-acid acylase;
7beta-(4-carboxybutanamido)cephalosporanic acid acylase;
cephalosporin C acylase;
glutaryl-7-ACA acylase;
CA;
GCA;
GA;
cephalosporin acylase;
glutaryl-7-aminocephalosporanic acid acylase;
GL-7-ACA acylase
|
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| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
 |
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| Sysname |
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase
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| Reaction(IUBMB) |
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7-aminocephalosporanate + glutarate [RN: R07402]
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| Reaction(KEGG) |
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| Substrate |
(7R)-7-(4-carboxybutanamido)cephalosporanate [CPD: C15666];
H2O [CPD: C00001]
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| Product |
(7R)-7-aminocephalosporanate [CPD: C07756];
glutarate [CPD: C00489]
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| Comment |
Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.
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| Pathway |
| Penicillin and cephalosporin biosynthesis |
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| Orthology |
| glutaryl-7-aminocephalosporanic-acid acylase |
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| Reference |
|
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| Authors |
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K. |
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| Title |
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176. |
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| Journal |
Eur. J. Biochem. 230 (1995) 773-8. |
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| Organism |
Pseudomonas sp. |
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| Reference |
|
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| Authors |
Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M. |
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| Title |
Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 56 ( Pt 4) (2000) 458-9. |
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| Organism |
Pseudomonas sp. |
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| Reference |
|
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| Authors |
Monti D, Carrea G, Riva S, Baldaro E, Frare G. |
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| Title |
Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase. |
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| Journal |
Biotechnol. Bioeng. 70 (2000) 239-44. |
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| Reference |
|
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| Authors |
Kwon TH, Rhee S, Lee YS, Park SS, Kim KH. |
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| Title |
Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16. |
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| Journal |
J. Struct. Biol. 131 (2000) 79-81. |
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| Organism |
Pseudomonas sp. |
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| Reference |
|
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| Authors |
Kim Y, Yoon K, Khang Y, Turley S, Hol WG. |
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| Title |
The 2.0 A crystal structure of cephalosporin acylase. |
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| Journal |
Structure. Fold. Des. 8 (2000) 1059-68. |
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| Organism |
Pseudomonas diminuta |
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| Reference |
|
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| Authors |
Huang X, Zeng R, Ding X, Mao X, Ding Y, Rao Z, Xie Y, Jiang W, Zhao G. |
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| Title |
Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130. |
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| Journal |
J. Biol. Chem. 277 (2002) 10256-64. |
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| Organism |
Pseudomonas sp. |
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| Reference |
|
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| Authors |
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH. |
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| Title |
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation. |
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| Journal |
Biochemistry. 42 (2003) 4084-93. |
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| Organism |
Pseudomonas sp. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 56645-46-6 |
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