KEGG   ENZYME: 3.5.1.93Help
Entry
EC 3.5.1.93                 Enzyme                                 

Name
glutaryl-7-aminocephalosporanic-acid acylase;
7beta-(4-carboxybutanamido)cephalosporanic acid acylase;
cephalosporin C acylase;
glutaryl-7-ACA acylase;
CA;
GCA;
GA;
cephalosporin acylase;
glutaryl-7-aminocephalosporanic acid acylase;
GL-7-ACA acylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase
Reaction(IUBMB)
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7-aminocephalosporanate + glutarate [RN:R07402]
Reaction(KEGG)
Substrate
(7R)-7-(4-carboxybutanamido)cephalosporanate [CPD:C15666];
H2O [CPD:C00001]
Product
(7R)-7-aminocephalosporanate [CPD:C07756];
glutarate [CPD:C00489]
Comment
Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.
History
EC 3.5.1.93 created 2005
Pathway
Penicillin and cephalosporin biosynthesis
Orthology
K12748  
glutaryl-7-aminocephalosporanic-acid acylase
Reference
1  [PMID:7607251]
  Authors
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K.
  Title
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.
  Journal
Eur. J. Biochem. 230 (1995) 773-8.
  Organism
Pseudomonas sp.
Reference
2  [PMID:10739919]
  Authors
Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M.
  Title
Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 56 ( Pt 4) (2000) 458-9.
  Organism
Pseudomonas sp.
Reference
3  [PMID:10972935]
  Authors
Monti D, Carrea G, Riva S, Baldaro E, Frare G.
  Title
Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.
  Journal
Biotechnol. Bioeng. 70 (2000) 239-44.
Reference
4  [PMID:10945972]
  Authors
Kwon TH, Rhee S, Lee YS, Park SS, Kim KH.
  Title
Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.
  Journal
J. Struct. Biol. 131 (2000) 79-81.
  Organism
Pseudomonas sp.
Reference
5  [PMID:11080627]
  Authors
Kim Y, Yoon K, Khang Y, Turley S, Hol WG.
  Title
The 2.0 A crystal structure of cephalosporin acylase.
  Journal
Structure. Fold. Des. 8 (2000) 1059-68.
  Organism
Pseudomonas diminuta
  Sequence
[up:Q9L5D6]
Reference
6  [PMID:11782466]
  Authors
Huang X, Zeng R, Ding X, Mao X, Ding Y, Rao Z, Xie Y, Jiang W, Zhao G.
  Title
Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130.
  Journal
J. Biol. Chem. 277 (2002) 10256-64.
  Organism
Pseudomonas sp.
Reference
7  [PMID:12680762]
  Authors
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH.
  Title
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.
  Journal
Biochemistry. 42 (2003) 4084-93.
  Organism
Pseudomonas sp.
  Sequence
[up:P07662]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
56645-46-6

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