KEGG   ENZYME: 3.5.1.93Help
Entry
EC 3.5.1.93                 Enzyme                                 

Name
glutaryl-7-aminocephalosporanic-acid acylase;
7beta-(4-carboxybutanamido)cephalosporanic acid acylase;
cephalosporin C acylase;
glutaryl-7-ACA acylase;
CA;
GCA;
GA;
cephalosporin acylase;
glutaryl-7-aminocephalosporanic acid acylase;
GL-7-ACA acylase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase
Reaction(IUBMB)
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O = (7R)-7-aminocephalosporanate + glutarate [RN:R07402]
Reaction(KEGG)
Substrate
(7R)-7-(4-carboxybutanamido)cephalosporanate [CPD:C15666];
H2O [CPD:C00001]
Product
(7R)-7-aminocephalosporanate [CPD:C07756];
glutarate [CPD:C00489]
Comment
Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.
History
EC 3.5.1.93 created 2005
Pathway
Penicillin and cephalosporin biosynthesis
Biosynthesis of antibiotics
Orthology
K12748  
glutaryl-7-aminocephalosporanic-acid acylase
Reference
1  [PMID:7607251]
  Authors
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K.
  Title
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.
  Journal
Eur. J. Biochem. 230 (1995) 773-8.
Reference
2  [PMID:10739919]
  Authors
Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M.
  Title
Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 56 ( Pt 4) (2000) 458-9.
Reference
3  [PMID:10972935]
  Authors
Monti D, Carrea G, Riva S, Baldaro E, Frare G.
  Title
Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.
  Journal
Biotechnol. Bioeng. 70 (2000) 239-44.
Reference
4  [PMID:10945972]
  Authors
Kwon TH, Rhee S, Lee YS, Park SS, Kim KH.
  Title
Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.
  Journal
J. Struct. Biol. 131 (2000) 79-81.
Reference
5  [PMID:11080627]
  Authors
Kim Y, Yoon K, Khang Y, Turley S, Hol WG.
  Title
The 2.0 A crystal structure of cephalosporin acylase.
  Journal
Structure. Fold. Des. 8 (2000) 1059-68.
  Sequence
[up:Q9L5D6]
Reference
6  [PMID:11782466]
  Authors
Huang X, Zeng R, Ding X, Mao X, Ding Y, Rao Z, Xie Y, Jiang W, Zhao G.
  Title
Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130.
  Journal
J. Biol. Chem. 277 (2002) 10256-64.
Reference
7  [PMID:12680762]
  Authors
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH.
  Title
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.
  Journal
Biochemistry. 42 (2003) 4084-93.
  Sequence
[up:P07662]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
56645-46-6

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