KEGG   ENZYME: 3.5.2.1Help
Entry
EC 3.5.2.1                  Enzyme                                 

Name
barbiturase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
BRITE hierarchy
Sysname
barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming)
Reaction(IUBMB)
barbiturate + H2O = 3-oxo-3-ureidopropanoate [RN:R02139]
Reaction(KEGG)
R02139;
(other) R03870 R10777
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Substrate
barbiturate [CPD:C00813];
H2O [CPD:C00001]
Product
3-oxo-3-ureidopropanoate [CPD:C15607]
Comment
Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase.
History
EC 3.5.2.1 created 1961, modified 2006
Pathway
Pyrimidine metabolism
Orthology
K19794  
barbiturase
Genes
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
ICA: 
NCA: 
PSIM: 
SRO: 
NML: 
GOB: 
BSD: 
MMAR: 
SVI: 
PSEH: 
CAI: 
TBI: 
 » show all
Taxonomy
Reference
1  [PMID:12981104]
  Authors
HAYAISHI O, KORNBERG A.
  Title
Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes.
  Journal
J. Biol. Chem. 197 (1952) 717-32.
Reference
2  [PMID:11485332]
  Authors
Soong CL, Ogawa J, Shimizu S.
  Title
Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase.
  Journal
Biochem. Biophys. Res. Commun. 286 (2001) 222-6.
  Sequence
Reference
3  [PMID:11748240]
  Authors
Soong CL, Ogawa J, Sakuradani E, Shimizu S.
  Title
Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism.
  Journal
J. Biol. Chem. 277 (2002) 7051-8.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9025-16-5

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