KEGG   ENZYME: 3.5.2.15Help
Entry
EC 3.5.2.15                 Enzyme                                 

Name
cyanuric acid amidohydrolase;
AtzD
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
BRITE hierarchy
Sysname
cyanuric acid amidohydrolase
Reaction(IUBMB)
cyanuric acid + H2O = biuret + CO2 [RN:R07305]
Reaction(KEGG)
R07305;
(other) R05561
Show
Substrate
cyanuric acid [CPD:C06554];
H2O [CPD:C00001]
Product
biuret [CPD:C06555];
CO2 [CPD:C00011]
Comment
Along with EC 3.5.1.54 (allophanate hydrolase) and EC 3.5.1.84 (biuret amidohydrolase), this enzyme forms part of the cyanuric-acid metabolism pathway, which degrades s-triazide herbicides, such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine], in bacteria. This is a key enzyme in the pathway, catalysing the ring cleavage of cyanuric acid. The enzyme is specific for cyanuric acid as substrate as neither the structurally related compounds ammeline (2,4-diamino-6-hydroxy-s-triazine) and ammelide (2-amino-4,6-dihydroxy-s-triazine) nor a number of pyrimidine compounds, such as uracil and cytosine, can act as substrates [3].
History
EC 3.5.2.15 created 2000, modified 2008
Pathway
Atrazine degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K03383  
cyanuric acid amidohydrolase
Genes
PPSE: 
BN5_3198(trzD)
ADI: 
VPD: 
AVI: 
RLE: 
pRL100353(atzD)
RLG: 
RLB: 
RTR: 
RHL: 
NGL: 
NGG: 
BJA: 
blr7281(atzD)
BJU: 
BJP: 
BRA: 
BRADO6319(atzD)
BBT: 
BBta_1315(atzD)
BRS: 
S23_59060(atzD)
AOL: 
BRC: 
AZC: 
MRD: 
MET: 
MOR: 
MAQU: 
RHZ: 
CID: 
AFE: 
AFR: 
ACU: 
AFI: 
BCO: 
BLE: 
PJD: 
PBD: 
PAEN: 
PAEQ: 
PAEA: 
SAY: 
SAP: 
MTA: 
GOR: 
FRI: 
PDX: 
GLP: 
 » show all
Taxonomy
Reference
1  [PMID:1991731]
  Authors
Eaton RW, Karns JS.
  Title
Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strains.
  Journal
J. Bacteriol. 173 (1991) 1363-6.
  Sequence
Reference
2  [PMID:1846859]
  Authors
Eaton RW, Karns JS.
  Title
Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227.
  Journal
J. Bacteriol. 173 (1991) 1215-22.
  Sequence
Reference
3  [PMID:10427042]
  Authors
Karns JS.
  Title
Gene sequence and properties of an s-triazine ring-cleavage enzyme from Pseudomonas sp. strain NRRLB-12227.
  Journal
Appl. Environ. Microbiol. 65 (1999) 3512-7.
  Sequence
Reference
4  [PMID:12788776]
  Authors
Fruchey I, Shapir N, Sadowsky MJ, Wackett LP.
  Title
On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP.
  Journal
Appl. Environ. Microbiol. 69 (2003) 3653-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
132965-78-7

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