| Entry |
|
|---|
| Name |
cyanuric acid amidohydrolase;
AtzD |
|---|
| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides
 |
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| Sysname |
cyanuric acid amidohydrolase |
|---|
| Reaction(IUBMB) |
cyanuric acid + H2O = biuret + CO2 [RN:R07305] |
|---|
| Reaction(KEGG) |
R07305;
(other) R05561
 |
|---|
| Substrate |
cyanuric acid [CPD:C06554];
H2O [CPD:C00001] |
|---|
| Product |
biuret [CPD:C06555];
CO2 [CPD:C00011] |
|---|
| Comment |
Along with EC 3.5.1.54 (allophanate hydrolase) and EC 3.5.1.84
(biuret amidohydrolase), this enzyme forms part of the cyanuric-acid
metabolism pathway, which degrades s-triazide herbicides, such as
atrazine
[2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine], in
bacteria. This is a key enzyme in the pathway, catalysing the ring
cleavage of cyanuric acid. The enzyme is specific for cyanuric acid
as substrate as neither the structurally related compounds ammeline
(2,4-diamino-6-hydroxy-s-triazine) and ammelide
(2-amino-4,6-dihydroxy-s-triazine) nor a number of pyrimidine
compounds, such as uracil and cytosine, can act as substrates [3]. |
|---|
| Pathway |
PATH: ec00791 Atrazine degradation
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K03383 cyanuric acid amidohydrolase |
|---|
| Genes |
AFE: Lferr_0922
AFR: AFE_0779
AVI: Avi_5653
RLE: pRL100353(atzD)
BJA: blr7281(atzD)
BRA: BRADO6319(atzD)
BBT: BBta_1315(atzD)
AZC: AZC_3892
MRD: Mrad2831_4776
MET: M446_3816
RHA: RHA1_ro05296
 |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:1991731]
Eaton RW, Karns JS.
Cloning and comparison of the DNA encoding ammelide aminohydrolase
and cyanuric acid amidohydrolase from three s-triazine-degrading
bacterial strains.
J. Bacteriol. 173 (1991) 1363-6.
Klebsiella pneumoniae [GN:kpn], Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:1846859]
Eaton RW, Karns JS.
Cloning and analysis of s-triazine catabolic genes from Pseudomonas
sp. strain NRRLB-12227.
J. Bacteriol. 173 (1991) 1215-22.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:10427042]
Karns JS.
Gene sequence and properties of an s-triazine ring-cleavage enzyme
from Pseudomonas sp. strain NRRLB-12227.
Appl. Environ. Microbiol. 65 (1999) 3512-7.
Pseudomonas sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:12788776]
Fruchey I, Shapir N, Sadowsky MJ, Wackett LP.
On the origins of cyanuric acid hydrolase: purification, substrates,
and prevalence of AtzD from Pseudomonas sp. strain ADP.
Appl. Environ. Microbiol. 69 (2003) 3653-7.
Pseudomonas sp. |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 3.5.2.15
IUBMB Enzyme Nomenclature: 3.5.2.15
ExPASy - ENZYME nomenclature database: 3.5.2.15
UM-BBD (Biocatalysis/Biodegradation Database): 3.5.2.15
BRENDA, the Enzyme Database: 3.5.2.15
CAS: 132965-78-7 |
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