KEGG   ENZYME: 3.5.3.21Help
Entry
EC 3.5.3.21                 Enzyme                                 
Name
methylenediurea deaminase;
methylenediurease
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amidines
BRITE hierarchy
Sysname
methylenediurea aminohydrolase
Reaction(IUBMB)
methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2 (overall reaction) [RN:R05169];
(1a) methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3 [RN:R05170];
(1b) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (spontaneous) [RN:R05171];
(1c) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (spontaneous) [RN:R05172]
Reaction(KEGG)
Substrate
methylenediurea [CPD:C06381];
H2O [CPD:C00001];
N-(carboxyaminomethyl)urea [CPD:C06382];
N-(aminomethyl)urea [CPD:C06383]
Product
N-(hydroxymethyl)urea [CPD:C06384];
NH3 [CPD:C00014];
CO2 [CPD:C00011];
N-(carboxyaminomethyl)urea [CPD:C06382];
N-(aminomethyl)urea [CPD:C06383]
Comment
Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
Reference
1
  Authors
Jahns, T., Schepp, R., Kaltwasser, H.
  Title
Purification and characterisation of an enzyme from a strain of Ochrobactrum anthropi that degrades condensation products of urea and formaldehyde (ureaform).
  Journal
Can. J. Microbiol. 43 (1997) 1111-1117.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
205830-62-2
DBGET integrated database retrieval system