KEGG   ENZYME: 3.5.5.8Help
Entry
EC 3.5.5.8                  Enzyme                                 

Name
thiocyanate hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles
BRITE hierarchy
Sysname
thiocyanate aminohydrolase
Reaction(IUBMB)
thiocyanate + 2 H2O = carbonyl sulfide + NH3 + HO- [RN:R05780]
Reaction(KEGG)
Substrate
thiocyanate [CPD:C01755];
H2O [CPD:C00001]
Product
carbonyl sulfide [CPD:C07331];
NH3 [CPD:C00014];
HO- [CPD:C01328]
Comment
The enzyme from Thiobacillus thioparus catalyses the first step in the degradation of thiocyanate.
Reference
1  [PMID:9573140]
  Authors
Katayama Y, Matsushita Y, Kaneko M, Kondo M, Mizuno T, Nyunoya H.
  Title
Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase.
  Journal
J. Bacteriol. 180 (1998) 2583-9.
  Organism
Thiobacillus thioparus
  Sequence
Reference
2  [PMID:1577754]
  Authors
Katayama Y, Narahara Y, Inoue Y, Amano F, Kanagawa T, Kuraishi H.
  Title
A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate.
  Journal
J. Biol. Chem. 267 (1992) 9170-5.
  Organism
Thiobacillus thioparus
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
142539-65-9

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