| Entry |
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| Name |
aminopyrimidine aminohydrolase;
thiaminase;
thiaminase II;
tenA (gene name)
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| Class |
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In other compounds
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| Sysname |
4-amino-5-aminomethyl-2-methylpyrimidine aminohydrolase
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| Reaction(IUBMB) |
(1) 4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia [RN: R09993];
(2) thiamine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole [RN: R02133]
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| Reaction(KEGG) |
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| Substrate |
4-amino-5-aminomethyl-2-methylpyrimidine [CPD: C20267];
H2O [CPD: C00001];
thiamine [CPD: C00378]
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| Product |
4-amino-5-hydroxymethyl-2-methylpyrimidine [CPD: C01279];
ammonia [CPD: C00014];
5-(2-hydroxyethyl)-4-methylthiazole [CPD: C04294]
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| Comment |
Previously known as thiaminase II, this enzyme is involved in the regeneration of the thiamine pyrimidine from degraded products, rather than in thiamine degradation, and participates in thiamine salvage pathways.
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| Pathway |
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| Orthology |
| thiaminase (transcriptional activator TenA) |
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| Genes |
OLU: | | OTA: | | EBT: | | EBI: | | EAE: | | EAR: | | KPN: | | KPU: | | KPM: | | KPP: | | KPE: | | KPO: | | KVA: | | KOX: | | SPE: | | SRR: | | SRS: | | SRA: | | SMW: | | PVA: | | PAO: | | EBF: | | HIN: | | HIT: | | HIP: | | HIQ: | | HIF: | | HIL: | | HIU: | | HIE: | | HIZ: | | HPR: | | PMU: | | PMV: | | PUL: | | PMP: | | APL: | | APJ: | | APA: | | GAN: | | VPA: | | VPB: | | VHA: | | VSP: | | VEX: | | VEJ: | | VFU: | | VFI: | | VFM: | | VSA: | | PPR: | | PPU: | | PPF: | | PPG: | | PPW: | | PPT: | | PPB: | | PPI: | | PPX: | | PPUH: | | ACI: | | ACD: | | ACB: | | ABM: | | ABY: | | ABC: | | ABN: | | ABB: | | ABX: | | ABR: | | ABD: | | ABH: | | ABAD: | | ACC: | | MAD: | | MBS: | | GPS: | | PIN: | | PSY: | | FNA: | | HEL: | | OCE: | | BPZ: | | AXY: | | CTT: | | HAR: | | MMS: | | HPY: | | HEO: | | HPJ: | | HPA: | | HPS: | | HHP: | | HHQ: | | HHR: | | HPG: | | HPP: | | HPB: | | HPL: | | HPC: | | HCA: | | HPM: | | HPE: | | HPO: | | HPI: | | HPQ: | | HPW: | | HPU: | | HEF: | | HPF: | | HEQ: | | HEX: | | HPT: | | HPZ: | | HPV: | | HPX: | | HEN: | | HPH: | | HEG: | | HPN: | | HEP: | | HEU: | | HES: | | HCN: | | HPD: | | HEY: | | HER: | | HEI: | | HPYA: | | HPYO: | | HPYL: | | HCM: | | CJE: | | CJB: | | CJJ: | | CJU: | | CJN: | | CJI: | | CJM: | | CJS: | | CJP: | | CJR: | | CJD: | | CLA: | | DPS: | | SUR: | | WOL: | | WRI: | | WPI: | | PEL: | | SFD: | | RET: | | REC: | | RLE: | | RLT: | | RLG: | | BME: | | BMI: | | BMZ: | | BMG: | | BMW: | | BMF: | | BMB: | | BMC: | | BAA: | | BMS: | | BSI: | | BMT: | | BSV: | | BOV: | | BCS: | | BSK: | | BMR: | | BPP: | | OAN: | | BJA: | | BJU: | | BRS: | | NWI: | | OCA: | | OCG: | | OCO: | | AZC: | | SNO: | | MEX: | | MEA: | | MDI: | | MCH: | | MRD: | | MPO: | | MNO: | | BID: | | SIL: | | SIT: | | RSP: | | RSH: | | RDE: | | RLI: | | PDE: | | DSH: | | PSF: | | OAT: | | OAR: | | GDI: | | GDJ: | | GXY: | | RRU: | | RRF: | | RCE: | | RPM: | | AZL: | | ALI: | | TMO: | | APB: | | PGV: | | BSU: | | BSR: | | BSL: | | BSH: | | BSY: | | BSS: | | BST: | | BSO: | | BSN: | | BSQ: | | BSUB: | | BSX: | | BLI: | | BLD: | | BAO: | | BAY: | | BAQ: | | BYA: | | BAMP: | | BAML: | | BAZ: | | BQL: | | BXH: | | BQY: | | BAMI: | | BAE: | | BHA: | | BAN: | | BAR: | | BAT: | | BAH: | | BAI: | | BAX: | | BAL: | | BCE: | | BCA: | | BCZ: | | BCR: | | BCB: | | BCU: | | BCG: | | BCQ: | | BCX: | | BNC: | | BCF: | | BCER: | | BCY: | | BTK: | | BTL: | | BTB: | | BTT: | | BTC: | | BTF: | | BTM: | | BTG: | | BTI: | | BTN: | | BTHT: | | BWE: | | BCL: | | BPU: | | BPF: | | BMQ: | | BMD: | | BMH: | | BSE: | | BCO: | | BCK: | | BAG: | | BJS: | | OIH: | | GKA: | | GTH: | | GTE: | | GWC: | | GYC: | | GYA: | | GCT: | | GMC: | | GGH: | | AFL: | | AXL: | | SAU: | | SAV: | | SAW: | | SAH: | | SAJ: | | SAM: | | SAS: | | SAR: | | SAC: | | SAA: | | SAO: | | SAE: | | SAD: | | SUU: | | SUV: | | SUH: | | SUE: | | SUJ: | | SUK: | | SUC: | | SUT: | | SUQ: | | SUZ: | | SUD: | | SUX: | | SUW: | | SUG: | | SUF: | | SAB: | | SUY: | | SAUB: | | SAUM: | | SEP: | | SER: | | SHA: | | SSP: | | SCA: | | SLG: | | SLN: | | SSD: | | SDT: | | SWA: | | LMO: | | LMF: | | LMH: | | LMC: | | LMN: | | LMY: | | LMT: | | LMG: | | LMS: | | LMJ: | | LMQ: | | LML: | | LMP: | | LMW: | | LMX: | | LMZ: | | LMON: | | LMOC: | | LMOS: | | LMOO: | | LMOY: | | LMOT: | | LMOA: | | LMOL: | | LMOG: | | LMOE: | | LIN: | | LWE: | | HHD: | | ESI: | | EAT: | | EAN: | | MCL: | | BBE: | | PJD: | | GYM: | | PPY: | | PPM: | | PMS: | | PMQ: | | PMW: | | PTA: | | AAC: | | AAD: | | SIV: | | LLA: | | LLK: | | LLT: | | LLS: | | LLC: | | LLM: | | LLR: | | LLN: | | LLI: | | SPN: | | SPD: | | SPR: | | SPW: | | SPX: | | SNE: | | SPV: | | SNM: | | SJJ: | | SPP: | | SNT: | | SNC: | | SNB: | | SNP: | | SNI: | | SNV: | | SNX: | | SND: | | SNU: | | SPNG: | | SAG: | | SAN: | | SAK: | | SGC: | | SAGS: | | SGA: | | SGG: | | SGT: | | SOR: | | STB: | | STD: | | SIF: | | LSA: | | LBR: | | LBK: | | LCA: | | LCB: | | LCZ: | | LCS: | | LCE: | | LCW: | | LFE: | | LRH: | | LRG: | | LRL: | | LRA: | | LBH: | | LBN: | | PPE: | | PCE: | | EFA: | | EFL: | | EFI: | | EFD: | | EFS: | | EHR: | | ENE: | | MPS: | | MPX: | | LME: | | LMM: | | LCI: | | LKI: | | LGS: | | LEC: | | LCN: | | LGE: | | WKO: | | CPE: | | CPF: | | CBK: | | CBN: | | CBT: | | CBE: | | CLJ: | | CSO: | | CAD: | | CCT: | | FPA: | | OVA: | | TMR: | | SAY: | | SAP: | | BPRS: | | NTH: | | HAS: | | MSM: | | MSG: | | MSA: | | MVA: | | MGI: | | MSP: | | MMC: | | MKM: | | MJL: | | MJD: | | MRH: | | ASD: | | NFA: | | NCY: | | NBR: | | RHA: | | RER: | | ROP: | | REQ: | | GOR: | | KSK: | | AAU: | | ARR: | | RSA: | | RMU: | | PPC: | | MPH: | | KFL: | | TFU: | | NDA: | | NAL: | | TCU: | | SRO: | | FRE: | | FAL: | | GOB: | | BSD: | | KRA: | | SEN: | | TBI: | | PDX: | | BLO: | | BLN: | | BLON: | | BLA: | | BLC: | | BLT: | | BBB: | | BBC: | | BNM: | | BLV: | | BLW: | | BLS: | | BNI: | | BDE: | | BBV: | | BBRU: | | GVG: | | GVH: | | RXY: | | CWO: | | PUV: | | LBI: | | LBF: | | BHY: | | BRM: | | BPO: | | BPJ: | | BPIP: | | BPW: | | BIP: | | SRU: | | SRM: | | RMR: | | RMG: | | BBD: | | EVI: | | DFE: | | SLI: | | LBY: | | FLI: | | ZPR: | | CAT: | | WVI: | | MRS: | | PTQ: | | STR: | | PSL: | | IPA: | | AMO: | | SYC: | | SYF: | | CYA: | | CYB: | | MAR: | | CYC: | | CYJ: | | HAO: | | GLP: | | ANA: | | NPU: | | NOP: | | AVA: | | ACY: | | CSG: | | CALO: | | CALT: | | RIV: | | PMM: | | PMI: | | PMB: | | PMC: | | PMG: | | PMH: | | TER: | | GEI: | | OAC: | | ONI: | | MIC: | | CTHE: | | PLP: | | SCS: | | CTS: | | RRS: | | RCA: | | CAU: | | CHL: | | HAU: | | TRO: | | STI: | | CAP: | | TRA: | | MSV: | | OPR: | | MHD: | | NDE: | | TTR: | | HMA: | | HHI: | | HWA: | | HWC: | | NPH: | | NMO: | | HLA: | | HUT: | | HTU: | | NMG: | | HVO: | | HME: | | HJE: | | HBO: | | HXA: | | NAT: | | NGE: | | HRU: | | NOU: | | PHO: | | PAB: | | PFU: | | PFI: | | PYN: | | PYS: | | TSI: | | APE: | | SMR: | | SHC: | | SSO: | | SOL: | | STO: | | SAI: | | SACN: | | SACR: | | SIS: | | SIA: | | SIM: | | SID: | | SIY: | | SIN: | | SII: | | SIH: | | SIR: | | SIC: | | MSE: | | MCN: | | AHO: | | PAI: | | PIS: | | PCL: | | PAS: | | PYR: | | POG: | | CMA: | | TNE: | | TUZ: | | TTN: | | VDI: | | VMO: | | FFO: | | HAH: | |
» show all
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| Reference |
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| Authors |
FUJITA A, NOSE Y, KURATANIK. |
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| Title |
The second type of bacterial thiaminase. |
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| Journal |
J. Vitaminol. (Kyoto). 1 (1954) 1-7. |
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| Organism |
Bacillus aneurinolyticus |
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| Reference |
2 |
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| Authors |
Ikehata, H. |
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| Title |
Purification of thiaminase II. |
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| Journal |
J. Gen. Appl. Microbiol. 6 (1960) 30-39. |
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| Reference |
|
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| Authors |
Toms AV, Haas AL, Park JH, Begley TP, Ealick SE |
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| Title |
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II. |
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| Journal |
Biochemistry. 44 (2005) 2319-29. |
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| Reference |
|
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| Authors |
Benach J, Edstrom WC, Lee I, Das K, Cooper B, Xiao R, Liu J, Rost B, Acton TB, Montelione GT, Hunt JF |
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| Title |
The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 61 (2005) 589-98. |
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| Reference |
|
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| Authors |
Jenkins AH, Schyns G, Potot S, Sun G, Begley TP |
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| Title |
A new thiamin salvage pathway. |
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| Journal |
Nat. Chem. Biol. 3 (2007) 492-7. |
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| Reference |
|
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| Authors |
Jenkins AL, Zhang Y, Ealick SE, Begley TP |
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| Title |
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis. |
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| Journal |
Bioorg. Chem. 36 (2008) 29-32. |
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| Reference |
|
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| Authors |
French JB, Begley TP, Ealick SE |
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| Title |
Structure of trifunctional THI20 from yeast. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 67 (2011) 784-91. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 9024-80-0 |
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