KEGG   ENZYME: 3.7.1.17Help
Entry
EC 3.7.1.17                 Enzyme                                 

Name
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase;
tesD (gene name);
hsaD (gene name)
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
BRITE hierarchy
Sysname
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming)
Reaction(IUBMB)
(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H2O = 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + (2Z,4Z)-2-hydroxyhexa-2,4-dienoate [RN:R09883]
Reaction(KEGG)
Substrate
(1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [CPD:C04844];
H2O [CPD:C00001]
Product
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
(2Z,4Z)-2-hydroxyhexa-2,4-dienoate [CPD:C20240]
Comment
The enzyme is involved in the bacterial degradation of the steroid ring structure, and is involved in degradation of multiple steroids, such as testosterone [1], cholesterol [2], and sitosterol.
History
EC 3.7.1.17 created 2012
Pathway
Steroid degradation
Orthology
K16050  
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Genes
PRE: 
PPUT: 
PPUN: 
PP4_27040(mhpC)
PPUU: 
PKC: 
PFZ: 
PPSY: 
SPL: 
SHL: 
PHA: 
PSHAa0893(tesD)
PTN: 
ZAL: 
GBI: 
RIN: 
REU: 
REH: 
H16_B0600(bphD)
CNC: 
CTI: 
CBW: 
BUR: 
BCJ: 
BCEN: 
BCEO: 
BCT: 
BCED: 
BPYR: 
BCON: 
BUL: 
BW21_2676(hsaD)
BPLA: 
BUD: 
BFN: 
PVE: 
CTT: 
CTES: 
THU: 
CHQ: 
SSAN: 
MTU: 
Rv3569c(hsaD)
MTV: 
MTC: 
MT3674(bphD)
MRA: 
MRA_3608(bphD)
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
CFBS_3786(bphD)
MTL: 
MTO: 
MTD: 
UDA_3569c(bphD)
MTN: 
MTJ: 
MTUB: 
MTUC: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
MTUU: 
MTQ: 
MBO: 
Mb3600c(bphD)
MBB: 
BCG_3634c(bphD)
MBT: 
JTY_3635(bphD)
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MAF_35820(bphD)
MCE: 
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MPA: 
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MUL_4140(bphD)
MVA: 
MGI: 
MSP: 
MAY: 
MABO: 
MAZ: 
MMC: 
MKM: 
MJL: 
MMI: 
MMAR_5064(bphD)
MRH: 
MMM: 
MCB: 
MLI: 
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
ASD: 
AS9A_0925(mhpC)
NFA: 
NFR: 
NCY: 
NBR: 
NNO: 
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
REQ: 
RPY: 
RAV: 
TPR: 
SALU: 
SALL: 
SVT: 
MIO: 
LMOI: 
NCA: 
NDK: 
I601_3469(hsaD_2)
PSIM: 
AMD: 
AMED_2523(mhpC)
AMN: 
AMM: 
AMES_2495(mhpC)
AMZ: 
B737_2496(mhpC)
AOI: 
AORI_2485(mhpC)
AJA: 
AMQ: 
ALU: 
KAL: 
KPHY: 
LED: 
ALL: 
STP: 
SAQ: 
AMS: 
AFS: 
SYNE: 
AWA: 
 » show all
Taxonomy
Reference
1  [PMID:16151114]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T
  Title
Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
  Journal
Appl. Environ. Microbiol. 71 (2005) 5275-81.
Reference
2  [PMID:17264217]
  Authors
Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD
  Title
A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 1947-52.
  Sequence
[mtu:Rv3569c]
Reference
3  [PMID:18097091]
  Authors
Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM
  Title
Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 64 (2008) 2-7.
  Sequence
[mtu:Rv3569c]
Reference
4  [PMID:19875455]
  Authors
Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E, Eltis LD
  Title
Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism.
  Journal
J. Biol. Chem. 285 (2010) 434-43.
  Sequence
[mtu:Rv3569c]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 

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