KEGG   ENZYME: 4.1.1.77Help
Entry
EC 4.1.1.77                 Enzyme                                 

Name
2-oxo-3-hexenedioate decarboxylase;
4-oxalocrotonate carboxy-lyase (misleading);
4-oxalocrotonate decarboxylase (misleading);
cnbF (gene name);
praD (gene name);
amnE (gene name);
nbaG (gene name);
xylI (gene name)
Class
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
BRITE hierarchy
Sysname
(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)
Reaction(IUBMB)
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2 [RN:R02602]
Reaction(KEGG)
R02602;
(other) R05374
Show
Substrate
(3E)-2-oxohex-3-enedioate
Product
2-oxopent-4-enoate [CPD:C00596];
CO2 [CPD:C00011]
Comment
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].
History
EC 4.1.1.77 created 1999, modified 2011, modified 2012
Pathway
Benzoate degradation
Dioxin degradation
Xylene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K01617  
2-oxo-3-hexenedioate decarboxylase
Genes
SLQ: 
PGE: 
PSD: 
PPSE: 
BN5_3617(nahK)
PPW: 
PPJ: 
PSC: 
PSTU: 
PBM: 
PKC: 
PKB_3258(amne1) PKB_3606(amne3)
PALK: 
AVN: 
AVL: 
AVD: 
ACX: 
SWD: 
MAD: 
MSR: 
CYQ: 
CZA: 
APAC: 
TBN: 
RSM: 
RSE: 
RPI: 
RPF: 
RPJ: 
REU: 
REH: 
H16_B0549(h16_B0549)
CNC: 
RME: 
Rmet_1318(dmpH) Rmet_5210(amnE)
CBW: 
BVI: 
BUR: 
BCJ: 
BCEN: 
DM39_4831(dmpH)
BCT: 
BCED: 
DM42_4990(dmpH)
BCON: 
BUG: 
BXE: 
Bxe_A1150(amnE)
BRH: 
BPX: 
BPY: 
PPK: 
PPNO: 
PRB: 
CDN: 
POL: 
AJS: 
VEI: 
VPD: 
CTT: 
ADN: 
ADK: 
MPT: 
JAG: 
GJA_235(dmpH)
LCH: 
AZO: 
azo1854(lapH) azo2430(nahK)
DAR: 
TMZ: 
RBU: 
ARC: 
MXA: 
MFU: 
MSD: 
CCX: 
SUR: 
ARA: 
RLE: 
BRA: 
BBT: 
MRD: 
MET: 
MSL: 
NAR: 
NPP: 
SWI: 
SJP: 
SJA_C1-11880(hpaH_xylI)
SYB: 
BLI: 
BLD: 
BLi03993(xylI)
BLH: 
BCA: 
BCX: 
BCF: 
BCER: 
BTL: 
BPF: 
BMQ: 
BMD: 
BACI: 
GTN: 
GTNG_3150(nbaG)
GTH: 
GYC: 
GYA: 
GCT: 
GMC: 
GGH: 
GJF: 
LGY: 
BBE: 
AAC: 
AAD: 
BTS: 
SAY: 
SAP: 
MVA: 
MJL: 
NFA: 
RHA: 
RER: 
ROP: 
REQ: 
REQ_05050(amnD)
GBR: 
SCB: 
ICA: 
NCA: 
TCU: 
SRO: 
FRI: 
NML: 
AMD: 
PDX: 
STP: 
SAQ: 
MAU: 
MIL: 
VMA: 
 » show all
Taxonomy
Reference
1  [PMID:1732207]
  Authors
Shingler V, Powlowski J, Marklund U.
  Title
Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600.
  Journal
J. Bacteriol. 174 (1992) 711-24.
  Sequence
Reference
2  [PMID:9683650]
  Authors
Takenaka S, Murakami S, Shinke R, Aoki K
  Title
Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway.
  Journal
Arch. Microbiol. 170 (1998) 132-7.
Reference
3  [PMID:10651637]
  Authors
Stanley TM, Johnson WH Jr, Burks EA, Whitman CP, Hwang CC, Cook PF
  Title
Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase.
  Journal
Biochemistry. 39 (2000) 718-26.
Reference
4  [PMID:17902707]
  Authors
Wang SC, Johnson WH Jr, Czerwinski RM, Stamps SL, Whitman CP
  Title
Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions.
  Journal
Biochemistry. 46 (2007) 11919-29.
Reference
5  [PMID:19717587]
  Authors
Kasai D, Fujinami T, Abe T, Mase K, Katayama Y, Fukuda M, Masai E
  Title
Uncovering the protocatechuate 2,3-cleavage pathway genes.
  Journal
J. Bacteriol. 191 (2009) 6758-68.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37325-55-6

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