KEGG   ENZYME: 4.1.1.77Help
Entry
EC 4.1.1.77                 Enzyme                                 
Name
2-oxo-3-hexenedioate decarboxylase;
4-oxalocrotonate carboxy-lyase (misleading);
4-oxalocrotonate decarboxylase (misleading);
cnbF (gene name);
praD (gene name);
amnE (gene name);
nbaG (gene name);
xylI (gene name)
Class
Lyases;
Carbon-carbon lyases;
Carboxy-lyases
BRITE hierarchy
Sysname
(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)
Reaction(IUBMB)
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2 [RN:R02602]
Reaction(KEGG)
R02602;
(other) R05374
Show
Substrate
(3E)-2-oxohex-3-enedioate
Product
2-oxopent-4-enoate [CPD:C00596];
CO2 [CPD:C00011]
Comment
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].
Pathway
Benzoate degradation
Dioxin degradation
Xylene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K01617  
4-oxalocrotonate decarboxylase
Genes
PSD: 
PPF: 
PPW: 
PPX: 
T1E_4272(tobG)
PSC: 
AVN: 
SPL: 
SHL: 
SWD: 
PHA: 
PSHAa2141(xylJ)
MAD: 
HP15_1116 HP15_4037
RSO: 
RS01663(RSp0892)
RPI: 
Rpic_4619
RPF: 
Rpic12D_3543
REU: 
Reut_A1510 Reut_B5220 Reut_B5499 Reut_B5500 Reut_B5692
REH: 
H16_B0549
RME: 
Rmet_1318(dmpH) Rmet_5210(amnE)
CNC: 
CNE_2c04950(dmpH) CNE_BB1p11980(xylI) CNE_BB1p13270(dmpH)
BVI: 
Bcep1808_7606
BUR: 
Bcep18194_B1459 Bcep18194_B2958 Bcep18194_C7643
BCJ: 
BCAM2123
BXE: 
Bxe_A1150(amnE)
BPY: 
Bphyt_1469
BUG: 
BC1001_0834
BRH: 
RBRH_00201
POL: 
Bpro_0971 Bpro_5137
AJS: 
Ajs_0225
VEI: 
Veis_2784
CTT: 
CtCNB1_3146
ADN: 
Alide_0340
ADK: 
Alide2_0285
MPT: 
Mpe_A2272 Mpe_A3324
LCH: 
Lcho_3342
AZO: 
azo1854(lapH) azo2430(nahK)
DAR: 
Daro_2773 Daro_3781
TMZ: 
Tmz1t_3090
ARC: 
ABLL_2441
MXA: 
MXAN_0925
SUR: 
STAUR_2722
ARA: 
Arad_7264 Arad_7266
RLE: 
pRL120704
BRA: 
BRADO5041
BBT: 
BBta_5513
MRD: 
Mrad2831_4067
MET: 
M446_1050
MSL: 
Msil_1480
NAR: 
Saro_3851
SWI: 
Swit_0912 Swit_3521
SJP: 
SJA_C1-11880(hpaH_xylI)
BLI: 
BL03907
BLD: 
BLi03993
BCA: 
BCE_2158
BCX: 
BCA_2156
BTL: 
BALH_1845
BPF: 
BpOF4_12615
BMQ: 
BMQ_3586
BMD: 
BMD_3571
GTN: 
GTNG_3150(nbaG)
GYC: 
GYMC61_2299
GYA: 
GYMC52_1427
GCT: 
GC56T3_2054
BBE: 
BBR47_29790
AAC: 
Aaci_1618
BTS: 
Btus_0970
SAY: 
TPY_0643
MTA: 
Moth_1777
MVA: 
Mvan_0595 Mvan_5236
MGI: 
Mflv_1520
MMC: 
Mmcs_4661 Mmcs_5436
MKM: 
Mkms_4749 Mkms_5833
MJL: 
Mjls_4208 Mjls_4209 Mjls_5044
NFA: 
nfa30430
RHA: 
RHA1_ro03880
RER: 
RER_04710 RER_59340
ROP: 
ROP_37490 ROP_54170
REQ: 
REQ_05050(amnD)
GBR: 
Gbro_0225
SCB: 
SCAB_15181
ICA: 
Intca_1093
NCA: 
Noca_1651 Noca_2055 Noca_2151
TCU: 
Tcur_0537
SRO: 
Sros_9112
FRI: 
FraEuI1c_1245
NML: 
Namu_0794
AMD: 
AMED_8622
PDX: 
Psed_4292
STP: 
Strop_3524 Strop_3527
SAQ: 
Sare_3899 Sare_3902
MAU: 
Micau_4937
MIL: 
ML5_3358
VMA: 
VAB18032_17780 VAB18032_28741
 » show all
Taxonomy
Reference
1  [PMID:1732207]
  Authors
Shingler V, Powlowski J, Marklund U.
  Title
Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600.
  Journal
J. Bacteriol. 174 (1992) 711-24.
  Organism
Pseudomonas putida, Pseudomonas sp.
  Sequence
NCBI-GI: 45685 (Pseudomonas sp. CF600)
Reference
2  [PMID:9683650]
  Authors
Takenaka S, Murakami S, Shinke R, Aoki K
  Title
Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway.
  Journal
Arch. Microbiol. 170 (1998) 132-7.
Reference
3  [PMID:10651637]
  Authors
Stanley TM, Johnson WH Jr, Burks EA, Whitman CP, Hwang CC, Cook PF
  Title
Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase.
  Journal
Biochemistry. 39 (2000) 718-26.
Reference
4  [PMID:17902707]
  Authors
Wang SC, Johnson WH Jr, Czerwinski RM, Stamps SL, Whitman CP
  Title
Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions.
  Journal
Biochemistry. 46 (2007) 11919-29.
Reference
5  [PMID:19717587]
  Authors
Kasai D, Fujinami T, Abe T, Mase K, Katayama Y, Fukuda M, Masai E
  Title
Uncovering the protocatechuate 2,3-cleavage pathway genes.
  Journal
J. Bacteriol. 191 (2009) 6758-68.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37325-55-6
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