D-threonine aldolase;
D-TA;
DTA;
low specificity D-TA;
low specificity D-threonine aldolase

Lyases;
Carbon-carbon lyases;
Aldehyde-lyases

D-threonine acetaldehyde-lyase (glycine-forming)

(1) D-threonine = glycine + acetaldehyde [RN:R08195];
(2) D-allothreonine = glycine + acetaldehyde [RN:R08196]

R08195 R08196

D-threonine [CPD:C00820];
D-allothreonine [CPD:C12317]

A pyridoxal-phosphate protein that is activated by divalent metal cations (e.g. Co2+, Ni2+, Mn2+ or Mg2+) [1,2]. The reaction is reversible, which can lead to the interconversion of D-threonine and D-allothreonine [1]. Several other D-beta-hydroxy-alpha-amino acids, such as D-beta-phenylserine, D-beta-hydroxy-alpha-aminovaleric acid and D-beta-3,4-dihydroxyphenylserine, can also act as substrate [1].

1  [PMID:9346293]

Kataoka M, Ikemi M, Morikawa T, Miyoshi T, Nishi K, Wada M, Yamada H, Shimizu S.

Isolation and characterization of D-threonine aldolase, a pyridoxal-5'-phosphate-dependent enzyme from Arthrobacter sp. DK-38.

Eur. J. Biochem. 248 (1997) 385-93.

2  [PMID:9642221]

Liu JQ, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H.

A novel metal-activated pyridoxal enzyme with a unique primary structure, low specificity D-threonine aldolase from Arthrobacter sp. Strain DK-38. Molecular cloning and cofactor characterization.

J. Biol. Chem. 273 (1998) 16678-85.

3  [PMID:10390816]

Liu JQ, Odani M, Dairi T, Itoh N, Shimizu S, Yamada H.

A new route to L-threo-3-[4-(methylthio)phenylserine], a key intermediate for the synthesis of antibiotics: recombinant low-specificity D-threonine aldolase-catalyzed stereospecific resolution.

Appl. Microbiol. Biotechnol. 51 (1999) 586-91.

4  [PMID:10952004]

Liu JQ, Odani M, Yasuoka T, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H.

Gene cloning and overproduction of low-specificity D-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug.

Appl. Microbiol. Biotechnol. 54 (2000) 44-51.

5

Liu, J.Q., Dairi, T., Itoh, N., Kataoka, M., Shimizu, S. and Yamada, H.

Diversity of microbial threonine aldolases and their application.

J. Mol. Catal. B 10 (2000) 107-115.

6  [PMID:12686135]

Paiardini A, Contestabile R, D'Aguanno S, Pascarella S, Bossa F.

Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes.

Biochim. Biophys. Acta. 1647 (2003) 214-9.