KEGG   ENZYME: 4.1.99.11Help
Entry
EC 4.1.99.11                Enzyme                                 

Name
benzylsuccinate synthase;
benzylsuccinate fumarate-lyase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
benzylsuccinate fumarate-lyase (toluene-forming)
Reaction(IUBMB)
benzylsuccinate = toluene + fumarate [RN:R05598]
Reaction(KEGG)
Substrate
benzylsuccinate [CPD:C09816]
Product
toluene [CPD:C01455];
fumarate [CPD:C00122]
Comment
A glycyl radical enzyme that is inhibited by benzyl alcohol, benzaldehyde, phenylhydrazine and is inactivated by oxygen.
History
EC 4.1.99.11 created 2000
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K07540  
benzylsuccinate synthase
Genes
EBA: 
c2A303(bssC) c2A304(bssA) c2A305(bssB)
AZI: 
GME: 
Gmet_1538(bssB) Gmet_1539(bssA) Gmet_1540(bssC)
GEO: 
Geob_2437(bssB-2) Geob_2438(bssA-2) Geob_2439(bssC-2) Geob_2447(bssB-1) Geob_2448(bssA-1) Geob_2449(bssC-1)
DAL: 
DTO: 
DGI: 
Taxonomy
Reference
1  [PMID:9765580]
  Authors
Beller HR, Spormann AM.
  Title
Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.
  Journal
J. Bacteriol. 180 (1998) 5454-7.
Reference
2  [PMID:9632263]
  Authors
Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J.
  Title
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism.
  Journal
Mol. Microbiol. 28 (1998) 615-28.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
209264-18-6

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