KEGG   ENZYME: 4.1.99.14Help
Entry
EC 4.1.99.14                Enzyme                                 

Name
spore photoproduct lyase;
SAM;
SP lyase;
SPL;
SplB;
SplG
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
spore photoproduct pyrimidine-lyase
Reaction(IUBMB)
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA) = thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Substrate
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
Product
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5'-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].
History
EC 4.1.99.14 created 2009, modified 2010
Orthology
K03716  spore photoproduct lyase
Genes
MMT: Metme_0388
MDN: JT25_009590
MDH: AYM39_19600
MKO: MKLM6_3962
MAH: MEALZ_0137
TEE: Tel_03165
GAI: IMCC3135_32475(splB)
SUA: Saut_0241
SKU: Sulku_0146
CHA: CHAB381_0074
CGRA: CGRAC_0580
NIS: NIS_0569
NAM: NAMH_0528
DMA: DMR_45420
DAS: Daes_2177
DPI: BN4_10012
PPRF: DPRO_0012
DBA: Dbac_2907
DRT: Dret_1935
DSF: UWK_02824
DOL: Dole_1936
DAL: Dalk_0910
BMX: BMS_1037
RFE: RF_0551
RRI: A1G_02660
RPK: RPR_06935
MEX: Mext_2717
MDI: METDI3483
MCH: Mchl_2944
MET: M446_5726
MPO: Mpop_2839
MOR: MOC_1042
META: Y590_13060
MAQU: Maq22A_c04270(splB)
STAX: MC45_09865
BSU: BSU13930(splB)
BSR: I33_1571(splB)
BSL: A7A1_1539
BSH: BSU6051_13930(splB)
BSUT: BSUB_01518(splB)
BSUL: BSUA_01518(splB)
BSUS: Q433_07995
BSS: BSUW23_07160(splB)
BST: GYO_1727(splB)
BSO: BSNT_07885(splB)
BSQ: B657_13930(splB)
BSX: C663_1436(spIB)
BLI: BL03571(splB)
BLD: BLi01602(splB)
BLH: BaLi_c16380(splB)
BAY: RBAM_013700(spIB)
BAQ: BACAU_1344(splB)
BYA: BANAU_1306(splB)
BAMP: B938_07140
BAML: BAM5036_1309(splB)
BAMA: RBAU_1353(splB)
BAMN: BASU_1332(splB)
BAMB: BAPNAU_2389(splB)
BAMT: AJ82_07880
BAMY: V529_13270(spIB)
BMP: NG74_01427(splB)
BAO: BAMF_1470(splB)
BAZ: BAMTA208_10180(splB)
BQL: LL3_01497(splB)
BXH: BAXH7_02077(spIB)
BQY: MUS_1476(splB)
BAMI: KSO_012520
BAMC: U471_13990
BAMF: U722_07305
BATR: TD68_04220
BHA: BH2808(splB)
BAN: BA_4429(splB)
BAR: GBAA_4429(splB)
BAT: BAS4109
BAH: BAMEG_4463(splB)
BAI: BAA_4445(splB)
BANT: A16_44240
BANR: A16R_60260
BANS: BAPAT_4247
BANV: DJ46_3114(splB)
BCE: BC4205
BCA: BCE_4278(splB)
BCZ: BCE33L3959(splB)
BCR: BCAH187_A4334(splB)
BCU: BCAH820_4225(splB)
BCG: BCG9842_B0919(splB)
BCQ: BCQ_3992(splB)
BCX: BCA_4313(splB)
BAL: BACI_c41720(splB)
BNC: BCN_4118
BCF: bcf_20900
BCER: BCK_14160
BTK: BT9727_3948(splB)
BTL: BALH_3810(splB)
BTB: BMB171_C3863(splB)
BTT: HD73_3502
BTHI: BTK_17260
BTC: CT43_CH4219(splB)
BTM: MC28_3498
BTG: BTB_c43470(splG)
BTW: BF38_5374(splB)
BWW: bwei_0732(splB)
BMYC: DJ92_1308(splG) DJ92_5435(splB)
BMYO: BG05_1812(splB)
BCL: ABC2488(splB)
BPU: BPUM_1283
BPUM: BW16_07225
BPUS: UP12_06720
BPF: BpOF4_01635(splB)
BMQ: BMQ_2226(splB)
BMD: BMD_2184(splB)
BMH: BMWSH_3013(splB)
BMEG: BG04_4595(splB)
BJS: MY9_1527
BACW: QR42_06580
BACP: SB24_02845
BACB: OY17_09945
BACO: OXB_0887
BACY: QF06_05995
BACL: BS34A_15490(splB)
BALM: BsLM_1482
BEO: BEH_06890
BGY: BGLY_1595(splB)
BKW: BkAM31D_16990(splB)
BKO: CKF48_19300(splB)
BALT: CFN77_07085(splB)
OIH: OB2284
GTN: GTNG_2348
GGH: GHH_c24920(splB)
AFL: Aflv_0922(splB)
ANM: GFC28_3756(splB)
AAMY: GFC30_2607(splB)
ANL: GFC29_1677(splB)
LSP: Bsph_2057
LYS: LBYS11_07245(splB)
LYB: C3943_08975(splB)
VNE: CFK40_16350(splB)
VPN: A21D_03910(splB)
BBE: BBR47_21270(splB)
PPY: PPE_02867
PPM: PPSC2_15205(splB)
PPO: PPM_3056(splB)
PPOL: X809_31595
PPQ: PPSQR21_030230(splB)
PPOY: RE92_21515
PMS: KNP414_02639(splG)
PMW: B2K_14645
PLV: ERIC2_c33130(splG)
PSAB: PSAB_16765
PRI: PRIO_4700(splB)
PSWU: SY83_19355
KYR: CVV65_01810(splB)
SIV: SSIL_0558
LFB: C1X05_16055(splB)
CAC: CA_C0699 CA_C2900(splB)
CAE: SMB_G0713(splB) SMB_G2936
CAY: CEA_G0710 CEA_G2907(splB)
CPE: CPE0724
CPF: CPF_0720(splB)
CTC: CTC_00900
CBO: CBO0216(spl)
CBA: CLB_0257(splB)
CBH: CLC_0272(splB)
CBY: CLM_0266(splB)
CBL: CLK_3398(splB)
CBK: CLL_A1824(splB)
CBB: CLD_0559(splB)
CBI: CLJ_B0264(splB)
CBN: CbC4_5006
CBT: CLH_1683(splB)
CBF: CLI_0281(splB)
CBM: CBF_0249
CBE: Cbei_4361
CBZ: Cbs_4361
CBEI: LF65_04885
CSR: Cspa_c46410(splB)
CBV: U729_346(splB)
CSQ: CSCA_1344
CLD: CLSPO_c02490(splB)
CACE: CACET_c37310(splB)
CTYK: CTK_C12610
CTAE: BGI42_07730(splB)
AMT: Amet_0662
AOE: Clos_0743
CCEL: CCDG5_1063(splB)
HSC: HVS_12925(splB)
CPY: Cphy_1777
CPRO: CPRO_16080(splB)
ERT: EUR_22690
ERA: ERE_18560
CDF: CD630_02750(splB)
PDC: CDIF630_00399(splB)
CDC: CD196_0292(spl)
CDL: CDR20291_0279(spl)
PDF: CD630DERM_02750(splB)
DSY: DSY2469
DHD: Dhaf_3628
DRM: Dred_0691
DAE: Dtox_0238
PTH: PTH_0212(SplB) PTH_2267(SplB)
DAU: Daud_0667
SGY: Sgly_1116
HMO: HM1_2342(spIB)
ELM: ELI_2764
OVA: OBV_20430
TTE: TTE2677(SplB)
THX: Thet_2384
TIT: Thit_2299
MTA: Moth_1928
TPZ: Tph_c28660(splB)
CSC: Csac_0382
ATE: Athe_2595
TTM: Tthe_2644
TSH: Tsac_0414
CAD: Curi_c06870(splB)
PUF: UFO1_0963
PFT: JBW_03484
RHB: NY08_4313
RFA: A3L23_02432(splG)
RHS: A3Q41_00934(splG)
RHU: A3Q40_03923(splG)
KFL: Kfla_4483
GOB: Gobs_4985
MMAR: MODMU_5497
KRA: Krad_4460
ACTN: L083_3843
AFS: AFR_15210
LBO: LBWT_7990
CTHE: Chro_1073
TRA: Trad_0283
WCH: wcw_0148
LIL: LA_0226
LIE: LIF_A0196
LIC: LIC_10195(splB)
LIS: LIL_10195(splB1)
LBJ: LBJ_0162(splB)
LBL: LBL_2921(splB)
LBF: LBF_0608(splB)
LST: LSS_04641
EPO: Epro_1021
ETI: RSTT_684
RSD: TGRD_724
FNU: FN0898
SLI: Slin_1066
FAE: FAES_3815
PMO: Pmob_1416
DTN: DTL3_1588
CABY: Cabys_4042
DTU: Dtur_0463
CTHI: THC_0740
MBAK: MSBR3_0651
MFC: BRM9_2365
FPL: Ferp_2353
 » show all
Taxonomy
Reference
1  [PMID:16829676]
  Authors
Chandor A, Berteau O, Douki T, Gasparutto D, Sanakis Y, Ollagnier-de-Choudens S, Atta M, Fontecave M
  Title
Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis.
  Journal
J. Biol. Chem. 281 (2006) 26922-31.
  Sequence
[bsu:BSU13930]
Reference
2  [PMID:16968710]
  Authors
Pieck JC, Hennecke U, Pierik AJ, Friedel MG, Carell T
  Title
Characterization of a new thermophilic spore photoproduct lyase from Geobacillus  stearothermophilus (SplG) with defined lesion containing DNA substrates.
  Journal
J. Biol. Chem. 281 (2006) 36317-26.
Reference
3  [PMID:16829680]
  Authors
Buis JM, Cheek J, Kalliri E, Broderick JB
  Title
Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily.
  Journal
J. Biol. Chem. 281 (2006) 25994-6003.
  Sequence
[bsu:BSU13930]
Reference
4  [PMID:19012397]
  Authors
Mantel C, Chandor A, Gasparutto D, Douki T, Atta M, Fontecave M, Bayle PA, Mouesca JM, Bardet M
  Title
Combined NMR and DFT studies for the absolute configuration elucidation of the spore photoproduct, a UV-induced DNA lesion.
  Journal
J. Am. Chem. Soc. 130 (2008) 16978-84.
Reference
5  [PMID:20405152]
  Authors
Silver SC, Chandra T, Zilinskas E, Ghose S, Broderick WE, Broderick JB
  Title
Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by  spore photoproduct lyase.
  Journal
J. Biol. Inorg. Chem. 15 (2010) 943-55.
  Sequence
[cac:CA_C0699]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.14
IUBMB Enzyme Nomenclature: 4.1.99.14
ExPASy - ENZYME nomenclature database: 4.1.99.14
BRENDA, the Enzyme Database: 4.1.99.14
CAS: 37290-70-3

DBGET integrated database retrieval system