KEGG   ENZYME: 4.1.99.20Help
Entry
EC 4.1.99.20                Enzyme                                 

Name
3-amino-4-hydroxybenzoate synthase;
3,4-AHBA synthase;
griH (gene name)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate hydro-lyase (cyclizing, 3-amino-4-hydroxybenzoate-forming)
Reaction(IUBMB)
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate = 3-amino-4-hydroxybenzoate + phosphate + 2 H2O [RN:R10469]
Reaction(KEGG)
Substrate
2-amino-4,5-dihydroxy-6-oxo-7-(phosphonooxy)heptanoate [CPD:C20644]
Product
3-amino-4-hydroxybenzoate [CPD:C12115];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
Requires Mn2+ for maximum activity. The reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate. In the bacterium Streptomyces griseus the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore.
History
EC 4.1.99.20 created 2013
Orthology
K17835  
3-amino-4-hydroxybenzoic acid synthase
Genes
BUR: 
CCE: 
DDL: 
HMO: 
HM1_1788(aroB)
GPO: 
SGR: 
SGR_4248(griH)
SCB: 
SBH: 
SVE: 
SRC: 
MPH: 
FRA: 
SESP: 
BN6_21370(griH1) BN6_63890(griH2)
KAL: 
ACTN: 
 » show all
Taxonomy
Reference
1  [PMID:17003031]
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S.
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J. Biol. Chem. 281 (2006) 36944-51.
  Sequence
[sgr:SGR_4248]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system