| Entry |
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| Name |
cyanase;
cyanate lyase;
cyanate hydrolase;
cyanate aminohydrolase;
cyanate C-N-lyase;
cyanate hydratase
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| Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
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| Sysname |
carbamate hydro-lyase
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| Reaction(IUBMB) |
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 (overall reaction) [RN: R10079];
(1a) cyanate + HCO3- + H+ = carbamate + CO2 [RN: R03546];
(1b) carbamate + H+ = NH3 + CO2 (spontaneous) [RN: R07316]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1) can be considered as the reverse of 'carbamate = cyanate + H2O', where this is assisted by reaction with bicarbonate and carbon dioxide (see mechanism above) [2], and hence is classified in sub-subclass 4.2.1. Bicarbonate functions as a recycling substrate [2].
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| Pathway |
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| Orthology |
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| Genes |
TSP: | | ATH: | | ALY: | | GMX: | | CSV: | | RCU: | | POP: | | VVI: | | OSA: | | DOSA: | | BDI: | | SBI: | | ZMA: | | SMO: | | PPP: | | NCR: | | SMP: | | PAN: | | MTM: | | MGR: | | FGR: | | NHE: | | VAL: | | SSL: | | BFU: | | ANI: | | NFI: | | AFM: | | AOR: | | ANG: | | AFV: | | ACT: | | PCS: | | CIM: | | CPW: | | PBL: | | URE: | | ABE: | | TVE: | | AJE: | | PNO: | | PTE: | | ZTR: | | TML: | | CNE: | | CNB: | | CGI: | | LBC: | | CCI: | | SCM: | | UMA: | | PTI: | | TPS: | | ECO: | | ECJ: | | ECD: | | ECOK: | | ECE: | | ECS: | | ECF: | | ETW: | | ELX: | | EOJ: | | EOI: | | EOH: | | EOK: | | ELR: | | ECX: | | ECW: | | ECM: | | ECY: | | ECR: | | ECK: | | ECT: | | EOC: | | EUM: | | ELO: | | ELH: | | ESO: | | ESM: | | ESL: | | ECL: | | EBR: | | EBD: | | EKO: | | EKF: | | EDH: | | EDJ: | | EUN: | | ELW: | | ELL: | | ELP: | | EBL: | | EBE: | | ECOA: | | PLU: | | PAY: | | ESC: | | EAE: | | EAR: | | SPE: | | SMAF: | | SMW: | | DDD: | | XNE: | | EBF: | | PAE: | | PAU: | | PAP: | | PAG: | | PAF: | | PNC: | | PDK: | | PSG: | | PPF: | | PPW: | | PPB: | | PPI: | | PPX: | | PST: | | PSB: | | PSP: | | PFL: | | PFO: | | PFC: | | PEN: | | PMK: | | PSA: | | PSZ: | | PSR: | | PSC: | | PSJ: | | PSH: | | PDR: | | CJA: | | PAT: | | SDE: | | MHC: | | MAD: | | MBS: | | AMC: | | AMAC: | | AMB: | | AMG: | | AMK: | | AMAA: | | ALT: | | GAG: | | GPS: | | PIN: | | TTU: | | MMT: | | MAH: | | TCX: | | MEJ: | | CYQ: | | AEH: | | TKM: | | HNA: | | ADI: | | MMW: | | MPC: | | TOL: | | CVI: | | RSO: | | RSL: | | RSN: | | RPI: | | RPF: | | REH: | | RME: | | CNC: | | BMA: | | BMV: | | BML: | | BMN: | | BPS: | | BPM: | | BPL: | | BPD: | | BPR: | | BPZ: | | BPQ: | | BTE: | | BVI: | | BUR: | | BCN: | | BCH: | | BCM: | | BCJ: | | BAM: | | BAC: | | BCT: | | BXE: | | BPY: | | BGL: | | BUG: | | BGD: | | BYI: | | BPX: | | PNU: | | BPT: | | RFR: | | POL: | | ACK: | | VEI: | | VAP: | | VPE: | | MPT: | | CFU: | | THI: | | DAR: | | TBD: | | MFA: | | ANT: | | DTI: | | BJA: | | BJU: | | BRA: | | BBT: | | BRS: | | RPA: | | RPB: | | RPE: | | RPT: | | RPX: | | NWI: | | NHA: | | AOL: | | XAU: | | AZC: | | MEX: | | MEA: | | MDI: | | MCH: | | MRD: | | MET: | | MPO: | | MNO: | | MSL: | | HDN: | | HMC: | | PHL: | | OAT: | | OAR: | | NPP: | | GOH: | | TMO: | | PGV: | | BSUB: | | BCG: | | BTM: | | BTI: | | BTN: | | BWE: | | AAC: | | BTS: | | CLJ: | | STH: | | CLE: | | COO: | | MTA: | | MPA: | | MAV: | | MIT: | | MIR: | | MIA: | | MID: | | MSM: | | MSG: | | MUL: | | MVA: | | MGI: | | MSP: | | MAB: | | MMV: | | MMC: | | MKM: | | MJL: | | MJD: | | MMI: | | MMM: | | MLI: | | REQ: | | GPO: | | GOR: | | SRT: | | SCT: | | SCY: | | SHY: | | SHO: | | SRO: | | FRI: | | SVI: | | SESP: | | GMA: | | NKO: | | MIN: | | SYN: | | SYZ: | | SYY: | | SYT: | | SYS: | | SYQ: | | SYW: | | SYC: | | SYF: | | SYD: | | SYE: | | SYG: | | SYR: | | SYX: | | SYP: | | SYNP: | | MAR: | | CYN: | | CYJ: | | AMR: | | CAN: | | CSN: | | DSL: | | HAO: | | GLP: | | CMP: | | ANA: | | NOP: | | AVA: | | ACY: | | PMM: | | PMN: | | PME: | | LEP: | | GEI: | | ONI: | | PSEU: | | CEP: | | MIC: | | ARP: | | CTHE: | | SCS: | | DGO: | | AAE: | | HTH: | | HTE: | | TAL: | | NDE: | | TID: | | STO: | | NGA: | |
» show all
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| Reference |
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| Authors |
Anderson PM. |
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| Title |
Purification and properties of the inducible enzyme cyanase. |
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| Journal |
Biochemistry. 19 (1980) 2882-8. |
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| Organism |
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| Reference |
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| Authors |
Johnson WV, Anderson PM. |
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| Title |
Bicarbonate is a recycling substrate for cyanase. |
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| Journal |
J. Biol. Chem. 262 (1987) 9021-5. |
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| Organism |
Escherichia coli [GN: eco] |
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| Reference |
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| Authors |
TAUSSIG A. |
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| Title |
The synthesis of the induced enzyme, ''cyanase'', in E. coli. |
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| Journal |
Biochim. Biophys. Acta. 44 (1960) 510-9. |
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| Organism |
Escherichia coli [GN: eco] |
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| Reference |
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| Authors |
Taussig A. |
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| Title |
Some properties of the induced enzyme cyanase. |
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| Journal |
Can. J. Biochem. 43 (1965) 1063-9. |
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| Reference |
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| Authors |
Anderson PM, Korte JJ, Holcomb TA. |
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| Title |
Reaction of the N-terminal methionine residues in cyanase with diethylpyrocarbonate. |
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| Journal |
Biochemistry. 33 (1994) 14121-5. |
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| Organism |
Escherichia coli [GN: eco] |
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| Reference |
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| Authors |
Kozliak EI, Fuchs JA, Guilloton MB, Anderson PM. |
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| Title |
Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate. |
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| Journal |
J. Bacteriol. 177 (1995) 3213-9. |
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| Organism |
Escherichia coli [GN: eco] |
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| Reference |
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| Authors |
Walsh MA, Otwinowski Z, Perrakis A, Anderson PM, Joachimiak A. |
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| Title |
Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site. |
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| Journal |
Structure. 8 (2000) 505-14. |
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| Organism |
Escherichia coli [GN: eco] |
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| Sequence |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: UM-BBD (Biocatalysis/Biodegradation Database): BRENDA, the Enzyme Database: CAS: 37289-24-0 |
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