| Entry |
|
|---|
| Name |
cyanase;
cyanate lyase;
cyanate hydrolase;
cyanate aminohydrolase;
cyanate C-N-lyase;
cyanate hydratase |
|---|
| Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
 |
|---|
| Sysname |
carbamate hydro-lyase |
|---|
| Reaction(IUBMB) |
(1) cyanate + HCO3- + 2 H+ = NH3 + 2 CO2 (overall reaction);
(2) (1a) cyanate + HCO3- + H+ = carbamate + CO2 [RN:R03546];
(3) (1b) carbamate + H+ = NH3 + CO2 (spontaneous) [RN:R07316] |
|---|
| Reaction(KEGG) |
R03546 R07316;
(other) R07315
 |
|---|
| Substrate |
cyanate [CPD:C01417];
HCO3- [CPD:C00288];
H+ [CPD:C00080];
carbamate [CPD:C01563] |
|---|
| Product |
NH3 [CPD:C00014];
CO2 [CPD:C00011];
carbamate [CPD:C01563] |
|---|
| Comment |
This enzyme, which is found in bacteria and plants, is used to
decompose cyanate, which can be used as the sole source of nitrogen
[6,7]. Reaction (1) can be considered as the reverse of 'carbamate =
cyanate + H2O', where this is assisted by reaction with bicarbonate
and carbon dioxide (see mechanism above) [2], and hence is
classified in sub-subclass 4.2.1. Bicarbonate functions as a
recycling substrate [2]. |
|---|
| Pathway |
PATH: ec00910 Nitrogen metabolism |
|---|
| Orthology |
KO: K01725 cyanate lyase |
|---|
| Genes |
ATH: AT3G23490(CYN)
POP: POPTR_833411
RCU: RCOM_0038590
VVI: 100245884(GSVIVT00020318001)
OSA: 4348870(Os10g0471300)
SBI: SORBI_01g019580(SORBIDRAFT_01g019580)
ZMA: 100284448(cl3683_1)
PPP: PHYPADRAFT_148136
NCR: NCU01258
PAN: PODANSg8018
MGR: MGG_02451
FGR: FG07458.1
ANI: AN7331.2
AFM: AFUA_2G16530
AOR: AO090102000190
ANG: An15g07070
AFV: AFLA_003110
PCS: Pc21g18920
NFI: NFIA_091790
CIM: CIMG_09477
URE: UREG_04839
SSL: SS1G_11485
BFU: BC1G_08946
CNE: CND04630
CNB: CNBD1690
LBC: LACBIDRAFT_174676
UMA: UM01973.1
ECO: b0340(cynS)
ECJ: JW0331(cynS)
ECD: ECDH10B_1353(cynS)
ECE: Z0436(cynS)
ECS: ECs0393
ECF: ECH74115_0413(cynS)
ETW: ECSP_0402(cynS)
ECW: EcE24377A_0365(cynS)
ECX: EcHS_A0405(cynS)
ECM: EcSMS35_0371(cynS)
ECY: ECSE_0365
ECL: EcolC_3285
ECK: EC55989_0347(cynS)
ECR: ECIAI1_0342(cynS)
ECT: ECIAI39_0338(cynS)
EUM: ECUMN_0383(cynS)
EBL: B21_00298(cynS)
EBD: ECBD_3317
EBR: ECB_00294(cynS)
EOH: ECO103_0322(cynS)
EOI: ECO111_0377(cynS)
EOJ: ECO26_0377(cynS)
PLU: plu0112(cynS)
PAY: PAU_00092(cynS)
SPE: Spro_1533
PAE: PA2052(cynS)
PAU: PA14_37965(cynS)
PAP: PSPA7_3236(cynS)
PAG: PLES_32691(cynS)
PPF: Pput_3239
PPW: PputW619_3092
PST: PSPTO_1930(cynS)
PSB: Psyr_3483
PSP: PSPPH_3410(cynS)
PFL: PFL_1490(cynS)
PFO: Pfl01_3369
PEN: PSEEN3599(cynS)
PSA: PST_3566(cynS)
CJA: CJA_0012(cynS)
PAT: Patl_3478
SDE: Sde_2824
AMC: MADE_02560 MADE_02561
PIN: Ping_0702
TTU: TERTU_4160(cynS)
TCX: Tcr_0040
AEH: Mlg_2586
HNA: Hneap_2215
MMW: Mmwyl1_3729
CVI: CV_1880(cynS)
RSO: RS02188(RSp1630)
RPI: Rpic_4911
RPF: Rpic12D_3834
REH: H16_B0046(cynS)
RME: Rmet_5864
BMA: BMA2466(cynS)
BMV: BMASAVP1_A0385(cynS)
BML: BMA10229_A1245(cynS)
BMN: BMA10247_3321(cynS)
BPS: BPSL2950(cynS)
BPM: BURPS1710b_3463(cynS)
BPL: BURPS1106A_3464(cynS)
BPD: BURPS668_3427(cynS)
BPR: GBP346_A3608(cynS)
BTE: BTH_I1198(cynS)
BVI: Bcep1808_6046
BUR: Bcep18194_B2815
BCN: Bcen_4778
BCH: Bcen2424_3389
BCM: Bcenmc03_4127
BCJ: BCAM0401(cynS)
BAM: Bamb_5946
BAC: BamMC406_5702
BXE: Bxe_B0547 Bxe_C0467
BPY: Bphyt_0220 Bphyt_2147
BGL: bglu_2g09670
PNU: Pnuc_0994
BPT: Bpet3621(cynS)
RFR: Rfer_3739
POL: Bpro_0289
VEI: Veis_3975
VAP: Vapar_1335
MPT: Mpe_A3484
DAR: Daro_1442
TBD: Tbd_1941
MFA: Mfla_2169
BJA: bll5731
BRA: BRADO2774(cynS)
BBT: BBta_5412(cynS)
RPA: RPA2115(cynS)
RPB: RPB_2057
RPE: RPE_4635
RPT: Rpal_2406
NWI: Nwi_1302
NHA: Nham_1631
XAU: Xaut_2620
AZC: AZC_0763
MEX: Mext_2769
MEA: Mex_1p2966(cynS)
MDI: METDI3535(cynS)
MRD: Mrad2831_4539
MET: M446_4815
MPO: Mpop_2892
MCH: Mchl_2996
MNO: Mnod_1707
MSL: Msil_0112
BCG: BCG9842_B0013(cynS)
BWE: BcerKBAB4_4912
AAC: Aaci_2827
STH: STH22
MTA: Moth_2054
MPA: MAP4098
MAV: MAV_4538(cynS)
MSM: MSMEG_5359(cynS)
MUL: MUL_0711(cynS)
MVA: Mvan_2755
MGI: Mflv_1997
MAB: MAB_0054c MAB_1028c
MMC: Mmcs_0717
MKM: Mkms_0731
MJL: Mjls_0711
MMI: MMAR_0960(cynS)
SRO: Sros_4859
SVI: Svir_14070
MIN: Minf_0636(cynS)
SYN: slr0899(cynS)
SYW: SYNW2490(cynS)
SYC: syc1989_d(cynS)
SYF: Synpcc7942_2104
SYD: Syncc9605_2659
SYE: Syncc9902_2287
SYG: sync_2901(cynS)
SYR: SynRCC307_2485(cynS)
SYX: SynWH7803_2495(cynS)
SYP: SYNPCC7002_A0643(cynS) SYNPCC7002_A0863(cynS)
MAR: MAE_10370(cynS)
CYN: Cyan7425_0296
ANA: all1291(cynS)
AVA: Ava_3015
PMM: PMM0373(cynS)
PMN: PMN2A_1390
PME: NATL1_00701(cynS)
AMR: AM1_5165(cynS)
AAE: aq_212(cynS)
STO: ST1023
 |
|---|
| Structures |
PDB: 2IU7 2IUO 2IV1 2IVB 2IVG 2IVQ |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:6994799]
Anderson PM.
Purification and properties of the inducible enzyme cyanase.
Biochemistry. 19 (1980) 2882-8.
Escherichia coli [GN:eco ece] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:3110153]
Johnson WV, Anderson PM.
Bicarbonate is a recycling substrate for cyanase.
J. Biol. Chem. 262 (1987) 9021-5.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:13775509]
TAUSSIG A.
The synthesis of the induced enzyme, ''cyanase'', in E. coli.
Biochim. Biophys. Acta. 44 (1960) 510-9.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
|
4 [PMID:5322950]
Taussig A.
Some properties of the induced enzyme cyanase.
Can. J. Biochem. 43 (1965) 1063-9. |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:7947823]
Anderson PM, Korte JJ, Holcomb TA.
Reaction of the N-terminal methionine residues in cyanase with
diethylpyrocarbonate.
Biochemistry. 33 (1994) 14121-5.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
Organism
|
6 [PMID:7768821]
Kozliak EI, Fuchs JA, Guilloton MB, Anderson PM.
Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth
by cyanate.
J. Bacteriol. 177 (1995) 3213-9.
Escherichia coli [GN:eco] |
|---|
Reference
Authors
Title
Journal
Organism
Sequence
|
7 [PMID:10801492]
Walsh MA, Otwinowski Z, Perrakis A, Anderson PM, Joachimiak A.
Structure of cyanase reveals that a novel dimeric and decameric
arrangement of subunits is required for formation of the enzyme
active site.
Structure. 8 (2000) 505-14.
Escherichia coli [GN:eco]
ECO: b0340 |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 4.2.1.104
IUBMB Enzyme Nomenclature: 4.2.1.104
ExPASy - ENZYME nomenclature database: 4.2.1.104
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.104
BRENDA, the Enzyme Database: 4.2.1.104
CAS: 37289-24-0 |
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