KEGG   ENZYME: 4.2.1.106Help
Entry
EC 4.2.1.106                Enzyme                                 

Name
bile-acid 7alpha-dehydratase;
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate hydro-lyase (12alpha-hydroxy-3-oxochola-4,6-dienoate-forming)
Reaction(IUBMB)
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate = 12alpha-hydroxy-3-oxochola-4,6-dienoate + H2O [RN:R07318]
Reaction(KEGG)
Substrate
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate [CPD:C15568]
Product
12alpha-hydroxy-3-oxochola-4,6-dienoate [CPD:C15569];
H2O [CPD:C00001]
Comment
The enzyme from Eubacterium sp. strain VPI 12708 can also use 7alpha-hydroxy-3-oxochol-4-enoate as a substrate but not 7alpha,12alpha-dihydroxy-3-oxochol-5beta-anoate, 3alpha,7alpha,12alpha-trihydroxychol-5beta-anoate or 7beta-hydroxy-3-oxochol-4-enoate.
History
EC 4.2.1.106 created 2005
Pathway
Secondary bile acid biosynthesis
Orthology
K15872  
bile-acid 7alpha-dehydratase
Reference
1  [PMID:8808760]
  Authors
Dawson JA, Mallonee DH, Bjorkhem I, Hylemon PB.
  Title
Expression and characterization of a C24 bile acid 7 alpha-dehydratase from Eubacterium sp. strain VPI 12708 in Escherichia coli.
  Journal
J. Lipid. Res. 37 (1996) 1258-67.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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