KEGG   ENZYME: 4.2.1.119Help
Entry
EC 4.2.1.119                Enzyme                                 

Name
enoyl-CoA hydratase 2;
2-enoyl-CoA hydratase 2;
AtECH2;
ECH2;
MaoC;
MFE-2;
PhaJAc;
D-3-hydroxyacyl-CoA hydro-lyase;
D-specific 2-trans-enoyl-CoA hydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(3R)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O [RN:R09698]
Reaction(KEGG)
Substrate
(3R)-3-hydroxyacyl-CoA [CPD:C01086]
Product
(2E)-2-enoyl-CoA [CPD:C00658];
H2O [CPD:C00001]
Comment
This enzyme catalyses a hydration step in peroxisomal beta-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].
History
EC 4.2.1.119 created 2009
Orthology
K12405  
3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
K19658  
peroxisomal enoyl-CoA hydratase 2
K19659  
enoyl-CoA hydratase
Genes
HSA: 
3295(HSD17B4)
PTR: 
462019(HSD17B4)
PPS: 
100981028(HSD17B4)
GGO: 
101129538(HSD17B4)
PON: 
100448539(HSD17B4)
NLE: 
100590035(HSD17B4)
MCC: 
698369(HSD17B4)
MCF: 
102123675(HSD17B4)
CSAB: 
103244395(HSD17B4)
RRO: 
104654581(HSD17B4)
RBB: 
CJC: 
100390100(HSD17B4)
SBQ: 
101036776(HSD17B4)
MMU: 
15488(Hsd17b4)
RNO: 
79244(Hsd17b4)
CGE: 
NGI: 
103745113(Hsd17b4)
HGL: 
101713520(Hsd17b4)
CCAN: 
OCU: 
100346781(HSD17B4)
TUP: 
102468828(HSD17B4)
CFA: 
474630(HSD17B4)
AML: 
100471749(HSD17B4)
UMR: 
103670554(HSD17B4)
FCA: 
101091840(HSD17B4)
PTG: 
102968873(HSD17B4)
AJU: 
106975089(HSD17B4)
BTA: 
493643(HSD17B4)
BOM: 
102268517(HSD17B4)
BIU: 
PHD: 
102329357(HSD17B4)
CHX: 
102172142(HSD17B4)
OAS: 
101114519(HSD17B4)
SSC: 
397574(HSD17B4)
CFR: 
102508317(HSD17B4)
CDK: 
105104293(HSD17B4)
BACU: 
LVE: 
103084787(HSD17B4)
ECB: 
100034046(HSD17B4)
EPZ: 
103548315(HSD17B4)
EAI: 
106833039(HSD17B4)
MYB: 
102240475(HSD17B4)
MYD: 
102767159(HSD17B4)
HAI: 
109374101(HSD17B4)
RSS: 
109455167(HSD17B4)
PALE: 
102887000(HSD17B4)
LAV: 
100659299(HSD17B4)
MDO: 
100028098(HSD17B4)
SHR: 
100932771(HSD17B4)
OAA: 
100081754(HSD17B4)
GGA: 
395785(HSD17B4)
MGP: 
100545396(HSD17B4)
CJO: 
107306670(HSD17B4)
APLA: 
101791118(HSD17B4)
ACYG: 
106048362(HSD17B4)
TGU: 
100218226(HSD17B4)
GFR: 
102036502(HSD17B4)
FAB: 
101807233(HSD17B4)
PHI: 
102109547(HSD17B4)
CCW: 
104690497(HSD17B4)
FPG: 
101917400(HSD17B4)
FCH: 
102058406(HSD17B4)
CLV: 
102095771(HSD17B4)
ASN: 
102371766(HSD17B4)
AMJ: 
102571005(HSD17B4)
PSS: 
102451304(HSD17B4)
CMY: 
102933522(HSD17B4)
CPIC: 
101945395(HSD17B4)
ACS: 
100558484(hsd17b4)
PBI: 
103059748(SCP2D1) 103063870(HSD17B4)
GJA: 
107106522(SCP2D1) 107116225(HSD17B4)
XLA: 
444492(hsd17b4.L)
XTR: 
613082(hsd17b4)
NPR: 
108787696(HSD17B4)
DRE: 
393105(hsd17b4)
SRX: 
107713552(hsd17b4)
SANH: 
107703629(hsd17b4)
SGH: 
107571774(hsd17b4)
IPU: 
108265438(hsd17b4)
TRU: 
101062498(hsd17b4)
TNG: 
LCO: 
104920820(hsd17b4)
NCC: 
104966339(hsd17b4)
MZE: 
101482787(hsd17b4)
OLA: 
101159921(hsd17b4)
XMA: 
102223774(hsd17b4)
CSEM: 
103397642(hsd17b4)
LCF: 
108882142(hsd17b4)
SASA: 
106585849(hsd17b4)
ELS: 
105014609(hsd17b4)
SFM: 
108938133(hsd17b4)
LCM: 
102356548(HSD17B4)
CMK: 
103181119(hsd17b4)
BFO: 
CIN: 
100187421(hsd17b4)
SPU: 
581580(hsd17b4)
SKO: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSI: 
Dsimw501_GD29376(Dsim_GD29376)
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
AME: 
409986(GB18162)
BIM: 
BTER: 
SOC: 
AEC: 
ACEP: 
PBAR: 
HST: 
CFO: 
LHU: 
NVI: 
TCA: 
663655(17-beta-HD4)
DPA: 
NVL: 
BMOR: 
PXY: 
API: 
DNX: 
PHU: 
DPX: 
ISC: 
TUT: 
CEL: 
CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBR: 
CBG10899(Cbr-dhs-28) CBG13089(Cbr-maoc-1)
NAI: 
BMY: 
LOA: 
HRO: 
LGI: 
CRG: 
OBI: 
NVE: 
ADF: 
HMG: 
TAD: 
AQU: 
ATH: 
AT1G76150(ECH2)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
GHI: 
EGR: 
GMX: 
PVU: 
VRA: 
VAR: 
CCAJ: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj1g3v2268360.1(Lj1g3v2268360.1) Lj1g3v2268360.2(Lj1g3v2268360.2) Lj1g3v2953590.1(Lj1g3v2953590.1) Lj1g3v2953590.2(Lj1g3v2953590.2)
LANG: 
FVE: 
PPER: 
PMUM: 
MDM: 
ZJU: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0002s01520g(POPTRDRAFT_849471) POPTR_0005s26890g(POPTRDRAFT_652170)
VVI: 
SLY: 
SPEN: 
SOT: 
CANN: 
NTA: 
INI: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os09t0544900-01(Os09g0544900)
OBR: 
BDI: 
ATS: 
SBI: 
ZMA: 
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
FOX: 
PNO: 
MBR: 
SRE: 
DDI: 
DPP: 
DFA: 
DFA_02002(mfeA)
ACAN: 
TGO: 
TGME49_234570(HAD2SCP2)
TET: 
PTM: 
SMIN: 
v1.2.022875.t1(symbB.v1.2.022875.t1)
PIF: 
PSOJ: 
SPAR: 
NGR: 
SPL: 
SHL: 
FBL: 
MPUR: 
AHA: 
AHY: 
AHD: 
AHR: 
AHP: 
AHJ: 
AHH: 
AHI: 
ASA: 
ASA_2281(phaJ)
AMED: 
CARE: 
AGE: 
LLU: 
CBOT: 
 » show all
Taxonomy
Reference
1  [PMID:15644212]
  Authors
Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2.
  Journal
J. Mol. Biol. 345 (2005) 1157-69.
  Sequence
[hsa:3295]
Reference
2  [PMID:9457873]
  Authors
Fukui T, Shiomi N, Doi Y
  Title
Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae.
  Journal
J. Bacteriol. 180 (1998) 667-73.
  Sequence
Reference
3  [PMID:12832794]
  Authors
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T
  Title
Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2  domain of Candida tropicalis peroxisomal multifunctional enzyme type 2.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 59 (2003) 1302-5.
Reference
4  [PMID:11134939]
  Authors
Hisano T, Fukui T, Iwata T, Doi Y
  Title
Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 57 (2001) 145-7.
Reference
5  [PMID:16982622]
  Authors
Goepfert S, Hiltunen JK, Poirier Y
  Title
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana.
  Journal
J. Biol. Chem. 281 (2006) 35894-903.
  Sequence
[ath:AT1G76150]
Reference
6  [PMID:1879422]
  Authors
Engeland K, Kindl H
  Title
Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA.
  Journal
Eur. J. Biochem. 200 (1991) 171-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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