| Entry |
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| Name |
enoyl-CoA hydratase 2;
2-enoyl-CoA hydratase 2;
AtECH2;
ECH2;
MaoC;
MFE-2;
PhaJAc;
D-3-hydroxyacyl-CoA hydro-lyase;
D-specific 2-trans-enoyl-CoA hydratase
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| Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
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| Sysname |
(3R)-3-hydroxyacyl-CoA hydro-lyase
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| Reaction(IUBMB) |
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H2O [RN: R09698]
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| Reaction(KEGG) |
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| Substrate |
(3R)-3-hydroxyacyl-CoA [CPD: C01086]
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| Product |
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| Comment |
This enzyme catalyses a hydration step in peroxisomal beta-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].
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| Reference |
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| Authors |
Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T |
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| Title |
Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2. |
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| Journal |
J. Mol. Biol. 345 (2005) 1157-69. |
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| Reference |
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| Authors |
Fukui T, Shiomi N, Doi Y |
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| Title |
Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. |
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| Journal |
J. Bacteriol. 180 (1998) 667-73. |
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| Reference |
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| Authors |
Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T |
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| Title |
Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 59 (2003) 1302-5. |
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| Reference |
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| Authors |
Hisano T, Fukui T, Iwata T, Doi Y |
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| Title |
Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis. |
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| Journal |
Acta. Crystallogr. D. Biol. Crystallogr. 57 (2001) 145-7. |
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| Reference |
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| Authors |
Goepfert S, Hiltunen JK, Poirier Y |
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| Title |
Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana. |
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| Journal |
J. Biol. Chem. 281 (2006) 35894-903. |
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| Reference |
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| Authors |
Engeland K, Kindl H |
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| Title |
Evidence for a peroxisomal fatty acid beta-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(-)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA. |
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| Journal |
Eur. J. Biochem. 200 (1991) 171-8. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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