KEGG   ENZYME: 4.2.1.120Help
Entry
EC 4.2.1.120                Enzyme                                 

Name
4-hydroxybutanoyl-CoA dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
4-hydroxybutanoyl-CoA hydro-lyase
Reaction(IUBMB)
4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O [RN:R05337]
Reaction(KEGG)
Substrate
4-hydroxybutanoyl-CoA [CPD:C11062]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
H2O [CPD:C00001]
Comment
Contains FAD and a [4Fe-4S] iron-sulfur cluster. The enzyme has been characterized from several microorganisms, including Clostridium kluyveri, where it participates in succinate fermentation [1,2], Clostridium aminobutyricum, where it participates in 4-aminobutyrate degradation [3,4], and Metallosphaera sedula, where it participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [5].
History
EC 4.2.1.120 created 2009
Pathway
Butanoate metabolism
Carbon fixation pathways in prokaryotes
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K14534  
4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Genes
VPD: 
RTA: 
GME: 
DOL: 
DAL: 
DTO: 
SAT: 
DTI: 
SFU: 
DBR: 
OCG: 
OCO: 
CBE: 
CKL: 
CKL_3020(abfD)
CKR: 
CLS: 
CPAS: 
CDF: 
CDC: 
CDL: 
CDG: 
SLP: 
DDH: 
PTH: 
DOR: 
DAI: 
DMI: 
ELM: 
OVA: 
OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
CHY: 
CHY_1314(abfD)
MED: 
AIN: 
FNC: 
PGI: 
PG0692(abfD)
PGN: 
PGT: 
PAH: 
OSP: 
TFO: 
BFO_1484(abfD)
RSI: 
AFU: 
AF0333(hpaA-1)
AST: 
IHO: 
PFM: 
SSO: 
SSO2738(abfD-2)
SOL: 
STO: 
SAI: 
Saci_2143(abfD)
SACN: 
SACR: 
SACS: 
SIS: 
SIA: 
SIM: 
SID: 
SIY: 
SIN: 
SII: 
SIH: 
SIR: 
SIC: 
MSE: 
MCN: 
AHO: 
PAI: 
PIS: 
PCL: 
PYR: 
POG: 
TNE: 
TUZ: 
TTN: 
CLG: 
NMR: 
NIR: 
NKR: 
CSY: 
NGA: 
 » show all
Taxonomy
Reference
1  [PMID:13687513]
  Authors
BARTSCH RG, BARKER HA
  Title
A vinylacetyl isomerase from Clostridium kluyveri.
  Journal
Arch. Biochem. Biophys. 92 (1961) 122-32.
Reference
2  [PMID:8161284]
  Authors
Scherf U, Sohling B, Gottschalk G, Linder D, Buckel W
  Title
Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase.
  Journal
Arch. Microbiol. 161 (1994) 239-45.
  Organism
Clostridium kluyveri
Reference
3  [PMID:8344309]
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur. J. Biochem. 215 (1993) 421-9.
  Organism
Clostridium aminobutyricum
  Sequence
[up:P55792]
Reference
4  [PMID:8794752]
  Authors
Muh U, Cinkaya I, Albracht SP, Buckel W
  Title
4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction.
  Journal
Biochemistry. 35 (1996) 11710-8.
Reference
5  [PMID:18079405]
  Authors
Berg IA, Kockelkorn D, Buckel W, Fuchs G
  Title
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
  Journal
Science. 318 (2007) 1782-6.
  Organism
Metallosphaera sedula [GN:mse]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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