KEGG   ENZYME: 4.2.1.130Help
Entry
EC 4.2.1.130                Enzyme                                 
Name
D-lactate dehydratase;
glyoxylase III
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(R)-lactate hydro-lyase
Reaction(IUBMB)
(R)-lactate = methylglyoxal + H2O [RN:R09796]
Reaction(KEGG)
Substrate
(R)-lactate [CPD:C00256]
Product
methylglyoxal [CPD:C00546];
H2O [CPD:C00001]
Comment
The enzyme converts methylglyoxal to D-lactate in a single glutathione (GSH)-independent step. The other known route for this conversion is the two-step GSH-dependent pathway catalysed by EC 4.4.1.5 (lactoylglutathione lyase) and EC 3.1.2.6 (hydroxyacylglutathione hydrolase).
Reference
1  [PMID:7848303]
  Authors
Misra K, Banerjee AB, Ray S, Ray M
  Title
Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into D-lactate without reduced glutathione.
  Journal
Biochem. J. 305 ( Pt 3) (1995) 999-1003.
Reference
2  [PMID:21696459]
  Authors
Subedi KP, Choi D, Kim I, Min B, Park C
  Title
Hsp31 of Escherichia coli K-12 is glyoxalase III.
  Journal
Mol. Microbiol. 81 (2011) 926-36.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
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