KEGG   ENZYME: 4.2.1.140Help
Entry
EC 4.2.1.140                Enzyme                                 

Name
gluconate/galactonate dehydratase;
gluconate dehydratase (ambiguous);
Sso3198 (gene name);
Pto0485 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
D-gluconate/D-galactonate hydro-lyase
Reaction(IUBMB)
(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O [RN:R01538];
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O [RN:R03033]
Reaction(KEGG)
Substrate
D-gluconate [CPD:C00257];
D-galactonate [CPD:C00880]
Product
2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
H2O [CPD:C00001];
2-dehydro-3-deoxy-D-galactonate [CPD:C01216]
Comment
The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg2+ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.
History
EC 4.2.1.140 created 2013
Pathway
ec00030  Pentose phosphate pathway
ec00052  Galactose metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05308  gluconate/galactonate dehydratase
Genes
HAL: VNG_0442G(rspA)
HSL: OE_1664R(gnaD)
HHB: Hhub_1548(gnaD1)
HJE: HacjB3_13980
HMA: rrnAC0575(dgoA4)
HHI: HAH_1276
HHN: HISP_06530
HAB: SG26_14175
NMO: Nmlp_1390(gnaD)
HMU: Hmuk_2254
HWA: HQ_2412A(gnaD)
HWC: Hqrw_2676(gnaD)
HVO: HVO_1488(gnaD)
HME: HFX_1553(gnaD)
HLA: Hlac_1300
HTU: Htur_2654
NMG: Nmag_1145
NAT: NJ7G_1251
SALI: L593_03520
TVO: TVG0179275(TVG0179275)
PTO: PTO0485
SSO: SSO3198
SOL: Ssol_0935
SSOA: SULA_0968
SSOL: SULB_0970
SSOF: SULC_0969
STO: STK_23660(gnaD)
SAI: Saci_0885
SID: M164_2165
SII: LD85_2428
SIH: SiH_2107
SIR: SiRe_2039
SIC: SiL_2014
MSE: Msed_1303
MCN: Mcup_0900
AHO: Ahos_0261
PYR: P186_1932
CMA: Cmaq_0154
TUZ: TUZN_0958
TTN: TTX_1156(gad)
VDI: Vdis_1648
VMO: VMUT_2313
 » show all
Taxonomy
Reference
1  [PMID:15474024]
  Authors
Lamble HJ, Milburn CC, Taylor GL, Hough DW, Danson MJ
  Title
Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.
  Journal
FEBS. Lett. 576 (2004) 133-6.
  Sequence
[sso:SSO3198]
Reference
2  [PMID:15869466]
  Authors
Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B
  Title
The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.
  Journal
Biochem. J. 390 (2005) 529-40.
  Sequence
[sso:SSO3198]
Reference
3  [PMID:20023024]
  Authors
Reher M, Fuhrer T, Bott M, Schonheit P
  Title
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.
  Journal
J. Bacteriol. 192 (2010) 964-74.
  Sequence
[pto:PTO0485]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.140
IUBMB Enzyme Nomenclature: 4.2.1.140
ExPASy - ENZYME nomenclature database: 4.2.1.140
BRENDA, the Enzyme Database: 4.2.1.140

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