KEGG   ENZYME: 4.2.1.140Help
Entry
EC 4.2.1.140                Enzyme                                 

Name
gluconate/galactonate dehydratase;
gluconate dehydratase (ambiguous);
Sso3198 (gene name);
Pto0485 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
D-gluconate/D-galactonate hydro-lyase
Reaction(IUBMB)
(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O [RN:R01538];
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O [RN:R03033]
Reaction(KEGG)
Substrate
D-gluconate [CPD:C00257];
D-galactonate [CPD:C00880]
Product
2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
H2O [CPD:C00001];
2-dehydro-3-deoxy-D-galactonate [CPD:C01216]
Comment
The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg2+ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.
History
EC 4.2.1.140 created 2013
Pathway
Pentose phosphate pathway
Galactose metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K05308  
gluconate/galactonate dehydratase
Genes
HAL: 
VNG_0442G(rspA)
HSL: 
OE_1664R(gnaD)
HHB: 
Hhub_1548(gnaD1)
HJE: 
HMA: 
rrnAC0575(dgoA4)
HHI: 
HHN: 
HAB: 
NMO: 
Nmlp_1390(gnaD)
HMU: 
HWA: 
HQ_2412A(gnaD)
HWC: 
Hqrw_2676(gnaD)
HVO: 
HVO_1488(gnaD)
HME: 
HFX_1553(gnaD)
HGI: 
HBO: 
HLA: 
HTU: 
NMG: 
HXA: 
NAT: 
NPE: 
NGE: 
HRU: 
NOU: 
SALI: 
HLR: 
TVO: 
TVG0179275(TVG0179275)
PTO: 
FAC: 
SSO: 
SOL: 
SSOA: 
SSOL: 
SSOF: 
STO: 
STK_23660(gnaD)
SAI: 
SACN: 
SACR: 
SACS: 
SIS: 
SIA: 
SIM: 
SID: 
SIY: 
SIN: 
SII: 
SIH: 
SIR: 
SIC: 
SULA: 
MSE: 
MCN: 
AHO: 
PYR: 
CMA: 
TUZ: 
TTN: 
VDI: 
VMO: 
HAH: 
 » show all
Taxonomy
Reference
1  [PMID:15474024]
  Authors
Lamble HJ, Milburn CC, Taylor GL, Hough DW, Danson MJ
  Title
Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.
  Journal
FEBS. Lett. 576 (2004) 133-6.
  Sequence
[sso:SSO3198]
Reference
2  [PMID:15869466]
  Authors
Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B
  Title
The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.
  Journal
Biochem. J. 390 (2005) 529-40.
  Sequence
[sso:SSO3198]
Reference
3  [PMID:20023024]
  Authors
Reher M, Fuhrer T, Bott M, Schonheit P
  Title
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.
  Journal
J. Bacteriol. 192 (2010) 964-74.
  Sequence
[pto:PTO0485]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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