KEGG   ENZYME: 4.2.2.16Help
Entry
EC 4.2.2.16                 Enzyme                                 

Name
levan fructotransferase (DFA-IV-forming);
2,6-beta-D-fructan D-fructosyl-D-fructosyltransferase (forming di-beta-D-fructofuranose 2,6':2',6-dianhydride);
levan fructotransferase;
2,6-beta-D-fructan lyase (di-beta-D-fructofuranose-2,6':2',6-dianhydride-forming)
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
(2->6)-beta-D-fructan lyase (di-beta-D-fructofuranose-2,6':2',6-dianhydride-forming)
Reaction(IUBMB)
Produces di-beta-D-fructofuranose 2,6':2',6-dianhydride (DFA IV) by successively eliminating the diminishing (2->6)-beta-D-fructan (levan) chain from the terminal D-fructosyl-D-fructosyl disaccharide
Comment
This enzyme, like EC 4.2.2.17 [inulin fructotransferase (DFA-I-forming)] and EC 4.2.2.18 [inulin fructotransferase (DFA-III-forming)] eliminates the fructan chain from the terminal disaccharide leaving a difructose dianhydride. These enzymes have long been known as fructotransferases, so this is retained in the accepted name. Since the transfer is intramolecular, the reaction is an elimination and, hence, the enzyme is a lyase, belonging in EC 4.
History
EC 4.2.2.16 created 2004
Reference
1  [PMID:10899545]
  Authors
Song K, Bae K, Lee Y, Lee K, Rhee S
  Title
Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan.
  Journal
Enzyme. Microb. Technol. 27 (2000) 212-218.
Reference
2  [PMID:12696949]
  Authors
Jang KH, Ryu EJ, Park BS, Song KB, Kang SA, Kim CH, Uhm TB, Park YI, Rhee SK.
  Title
Levan fructotransferase from Arthrobacter oxydans J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan.
  Journal
J. Agric. Food. Chem. 51 (2003) 2632-6.
Reference
3  [PMID:10945246]
  Authors
Saito K, Tomita F.
  Title
Difructose anhydrides: their mass-production and physiological functions.
  Journal
Biosci. Biotechnol. Biochem. 64 (2000) 1321-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
88593-15-1

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