KEGG   ENZYME: 4.2.2.23Help
Entry
EC 4.2.2.23                 Enzyme                                 

Name
rhamnogalacturonan endolyase;
rhamnogalacturonase B;
alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase;
Rgase B;
rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase;
RG-lyase;
YesW;
RGL4;
Rgl11A;
Rgl11Y;
RhiE
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronate endolyase
Reaction(IUBMB)
Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Comment
The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168 and Aspergillus aculeatus.
History
EC 4.2.2.23 created 2011
Orthology
K18195  
rhamnogalacturonan endolyase
K18197  
rhamnogalacturonan endolyase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0002s11070g(POPTRDRAFT_551699) POPTR_0002s11080g(POPTRDRAFT_551700) POPTR_0002s11090g(POPTRDRAFT_551701) POPTR_0002s11100g(POPTRDRAFT_551702) POPTR_0005s19450g(POPTRDRAFT_716387) POPTR_0005s19460g(POPTRDRAFT_558976) POPTR_0006s23510g(POPTRDRAFT_1122718) POPTR_0014s01060g(POPTRDRAFT_834683) POPTR_0021s00440g
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
4346274(OJ1125_C01.16)
DOSA: 
Os08t0554100-01(Os08g0554100) Os08t0554300-00(Os08g0554300) Os11t0134100-00(Os11g0134100) Os12t0131900-00(Os12g0131900)
OBR: 
BDI: 
SBI: 
SORBI_05g001980(SORBIDRAFT_05g001980) SORBI_05g018090(SORBIDRAFT_05g018090) SORBI_07g024560(SORBIDRAFT_07g024560) SORBI_08g001440(SORBIDRAFT_08g001440) SORBI_09g030850(SORBIDRAFT_09g030850)
ZMA: 
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
PPA: 
CTEN: 
NCR: 
NCU05598(asd-1)
NTE: 
NEUTE1DRAFT118112(NEUTE1DRAFT_118112)
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
FPU: 
NHE: 
VAL: 
VDA: 
ELA: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_202064(AO090138000119) AOR_1_248194 AOR_1_590054(AO090011000349)
ANG: 
ANI_1_1554094(An11g00390) ANI_1_190124(An14g01130)
AFV: 
ACT: 
NFI: 
PCS: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
PFJ: 
NPA: 
TML: 
CNE: 
CNB: 
CGI: 
DSQ: 
SHS: 
HIR: 
PSQ: 
ADL: 
GTR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT120786(AGABI1DRAFT_120786)
ABV: 
AGABI2DRAFT72717(AGABI2DRAFT_72717)
PFP: 
PIF: 
PSOJ: 
ECA: 
ECA0804(rhiE)
PATR: 
PATO: 
PCT: 
PCC: 
PWA: 
PEC: 
SERR: 
DDD: 
DZE: 
DDC: 
DZC: 
CED: 
XCP: 
XCV: 
XAC: 
XAC3505(rhgB)
XCI: 
XCT: 
XCU: 
XCN: 
XCW: 
XCR: 
XCM: 
XCF: 
XCJ: 
XFU: 
XAX: 
XAO: 
XOO: 
XOO1078(rhgB)
XOM: 
XOO0978(XOO0978)
XOP: 
XOY: 
XOR: 
XAL: 
XSA: 
XTN: 
PFS: 
PPZ: 
GSN: 
AEX: 
SSAN: 
BSU: 
BSU07050(yesW)
BSR: 
BSL: 
BSH: 
BSY: 
BSUT: 
BSUL: 
BSUS: 
BSS: 
BST: 
BSO: 
BSN: 
BSQ: 
BSX: 
C663_0732(yesW)
BSP: 
BLI: 
BL03786(yesW)
BLD: 
BLi01376(yesW)
BLH: 
BAE: 
BATR: 
BJS: 
BACY: 
BACL: 
BALM: 
PPM: 
PPO: 
PPM_0906(M1_1103)
PPOL: 
PPQ: 
PPOY: 
PMS: 
PMQ: 
PMW: 
PTA: 
POD: 
PAEA: 
PPEO: 
LAW: 
MPS: 
MPX: 
CCB: 
CLB: 
CSB: 
CLT: 
CCE: 
CCL: 
CSD: 
COW: 
CKI: 
CKN: 
CLC: 
MAS: 
SMA: 
SCB: 
SDV: 
SCX: 
SRW: 
AMS: 
ASE: 
CCZ: 
OTE: 
OBT: 
VBA: 
GMA: 
FSC: 
BSA: 
BDO: 
BDH: 
BOA: 
BCEL: 
PDT: 
NKO: 
NSO: 
EVI: 
HYG: 
RUF: 
RTI: 
CIH: 
MRO: 
 » show all
Taxonomy
Reference
1  [PMID:8587995]
  Authors
Mutter M, Colquhoun IJ, Schols HA, Beldman G, Voragen AG
  Title
Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase.
  Journal
Plant. Physiol. 110 (1996) 73-7.
  Sequence
Reference
2  [PMID:8720076]
  Authors
Azadi P, O'Neill MA, Bergmann C, Darvill AG, Albersheim P
  Title
The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase.
  Journal
Glycobiology. 5 (1995) 783-9.
  Sequence
Reference
3  [PMID:9576783]
  Authors
Mutter M, Colquhoun IJ, Beldman G, Schols HA, Bakx EJ, Voragen AG
  Title
Characterization of recombinant rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin.
  Journal
Plant. Physiol. 117 (1998) 141-52.
  Sequence
Reference
4  [PMID:12136151]
  Authors
Kadirvelraj R, Harris P, Poulsen JC, Kauppinen S, Larsen S
  Title
A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus  aculeatus.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 58 (2002) 1346-9.
  Sequence
Reference
5  [PMID:12591882]
  Authors
Laatu M, Condemine G
  Title
Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi.
  Journal
J. Bacteriol. 185 (2003) 1642-9.
  Sequence
Reference
6  [PMID:12896991]
  Authors
Pages S, Valette O, Abdou L, Belaich A, Belaich JP
  Title
A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome.
  Journal
J. Bacteriol. 185 (2003) 4727-33.
Reference
7  [PMID:16682770]
  Authors
Ochiai A, Yamasaki M, Itoh T, Mikami B, Hashimoto W, Murata K
  Title
Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 62 (2006) 438-40.
  Sequence
[bsu:BSU07050]
Reference
8  [PMID:20851126]
  Authors
Jensen MH, Otten H, Christensen U, Borchert TV, Christensen LL, Larsen S, Leggio LL
  Title
Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan  lyase from Aspergillus aculeatus.
  Journal
J. Mol. Biol. 404 (2010) 100-11.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system