KEGG   ENZYME: 4.2.2.24Help
Entry
EC 4.2.2.24                 Enzyme                                 

Name
rhamnogalacturonan exolyase;
YesX
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronate exolyase
Reaction(IUBMB)
Exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I oligosaccharides containing alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The products are the disaccharide 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose and the shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Comment
The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168.
History
EC 4.2.2.24 created 2011
Orthology
K18198  rhamnogalacturonan exolyase
Genes
BSU: BSU07060(yesX)
BSR: I33_0797
BSL: A7A1_1750
BSH: BSU6051_07060(yesX)
BSY: I653_03565
BSUT: BSUB_00779(yesX)
BSUL: BSUA_00779(yesX)
BSUS: Q433_03975
BSS: BSUW23_03600(yesX)
BST: GYO_0966
BSQ: B657_07060(yesX)
BSX: C663_0733
BLI: BL03783(yesX)
BLD: BLi01379(yesX)
BLH: BaLi_c15200(yesX)
BATR: TD68_00740
BJS: MY9_0798
BACY: QF06_02435
BACL: BS34A_08050(yesX)
 » show all
Taxonomy
Reference
1  [PMID:19193638]
  Authors
Ochiai A, Itoh T, Mikami B, Hashimoto W, Murata K
  Title
Structural determinants responsible for substrate recognition and mode of action  in family 11 polysaccharide lyases.
  Journal
J. Biol. Chem. 284 (2009) 10181-9.
Reference
2  [PMID:17449691]
  Authors
Ochiai A, Itoh T, Kawamata A, Hashimoto W, Murata K
  Title
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
  Journal
Appl. Environ. Microbiol. 73 (2007) 3803-13.
  Sequence
[bsu:BSU07060]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.2.24
IUBMB Enzyme Nomenclature: 4.2.2.24
ExPASy - ENZYME nomenclature database: 4.2.2.24
BRENDA, the Enzyme Database: 4.2.2.24

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