KEGG   ENZYME: 4.2.2.25Help
Entry
EC 4.2.2.25                 Enzyme                                 

Name
gellan lyase
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
gellan beta-D-glucopyranosyl-(1->4)-D-glucopyranosyluronate lyase
Reaction(IUBMB)
Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end. The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp.
Comment
The enzyme is highly specific to gellan, especially deacetylated gellan.
History
EC 4.2.2.25 created 2011
Reference
1  [PMID:9056228]
  Authors
Hashimoto W, Maesaka K, Sato N, Kimura S, Yamamoto K, Kumagai H, Murata K
  Title
Microbial system for polysaccharide depolymerization: enzymatic route for gellan  depolymerization by Bacillus sp. GL1.
  Journal
Arch. Biochem. Biophys. 339 (1997) 17-23.
  Sequence
Reference
2  [PMID:9633595]
  Authors
Hashimoto W, Sato N, Kimura S, Murata K
  Title
Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1.
  Journal
Arch. Biochem. Biophys. 354 (1998) 31-9.
  Sequence
Reference
3  [PMID:14759609]
  Authors
Miyake O, Kobayashi E, Nankai H, Hashimoto W, Mikami B, Murata K
  Title
Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1.
  Journal
Arch. Biochem. Biophys. 422 (2004) 211-20.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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