KEGG   ENZYME: 4.2.3.132Help
Entry
EC 4.2.3.132                Enzyme                                 

Name
neoabietadiene synthase;
AgAS;
PtTPS-LAS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copaly-diphosphate diphosphate-lyase (cyclizing, neoabietadiene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = neoabietadiene + diphosphate [RN:R06303]
Reaction(KEGG)
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
neoabietadiene [CPD:C11880];
diphosphate [CPD:C00013]
Comment
Isolated from Abies grandis (grand fir) [1]. This class I enzyme forms about equal proportions of abietadiene, levopimaradiene and neoabietadiene. See also EC 4.2.3.18, abieta-7,13-diene synthase and EC 4.2.3.32, levopimaradiene synthase. An X-ray study of this multifunctional enzyme showed that the class I activity is in the alpha domain, while (+)-copalyl diphosphate synthase activity (EC 5.5.1.12, a class II activity) is in the beta and gamma domains [2]. In Pinus taeda (loblolly pine) the major product is levopimaradiene, with less abietadiene and neoabietadiene [3].
History
EC 4.2.3.132 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K12927  
abietadiene/neoabietadiene/copalyl diphosphate synthase
K19571  
levopimaradiene/neoabietadiene/copalyl diphosphate synthase
Reference
1  [PMID:11112547]
  Authors
Peters RJ, Flory JE, Jetter R, Ravn MM, Lee HJ, Coates RM, Croteau RB.
  Title
Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.
  Journal
Biochemistry. 39 (2000) 15592-602.
  Sequence
Reference
2  [PMID:22219188]
  Authors
Zhou K, Gao Y, Hoy JA, Mann FM, Honzatko RB, Peters RJ
  Title
Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.
  Journal
J. Biol. Chem. 287 (2012) 6840-50.
  Sequence
Reference
3  [PMID:16497345]
  Authors
Ro DK, Bohlmann J.
  Title
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).
  Journal
Phytochemistry. 67 (2006) 1572-8.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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