KEGG   ENZYME: 4.2.3.149Help
Entry
EC 4.2.3.149                Enzyme                                 

Name
nephthenol synthase;
DtcycA (gene name);
DtcycB (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
geranylgeranyl-diphosphate diphosphate-lyase [(R)-nephthenol-forming]
Reaction(IUBMB)
geranylgeranyl diphosphate + H2O = (R)-nephthenol + diphosphate [RN:R10897]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:C00353];
H2O [CPD:C00001]
Product
(R)-nephthenol [CPD:C20923];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. Two isozymes with this activity were isolated from the bacterium Streptomyces sp. SANK 60404. The enzyme encoded by the DtcycA gene also produces cembrene C (see EC 4.2.3.148, cembrene C synthase), while the enzyme encoded by the DtcycB gene also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase).
History
EC 4.2.3.149 created 2014
Orthology
K20504  
cembrene C/nephthenol synthase
K20505  
nephthenol/cembrene A/pentamethylcyclopentadecatrienol synthase
Reference
1  [PMID:23386483]
  Authors
Meguro A, Tomita T, Nishiyama M, Kuzuyama T
  Title
Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton.
  Journal
Chembiochem. 14 (2013) 316-21.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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