| Entry |
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| Name |
isoprene synthase;
ISPC;
ISPS
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| Class |
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
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| Sysname |
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
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| Reaction(IUBMB) |
dimethylallyl diphosphate = isoprene + diphosphate [RN: R08199]
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| Reaction(KEGG) |
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| Substrate |
dimethylallyl diphosphate [CPD: C00235]
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| Product |
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| Comment |
Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].
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| Pathway |
| Terpenoid backbone biosynthesis | | Biosynthesis of secondary metabolites |
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| Orthology |
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| Genes |
 |
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| Reference |
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| Authors |
Silver GM, Fall R. |
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| Title |
Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts. |
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| Journal |
Plant. Physiol. 97 (1991) 1588-1591. |
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| Reference |
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| Authors |
Silver GM, Fall R. |
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| Title |
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere. |
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| Journal |
J. Biol. Chem. 270 (1995) 13010-6. |
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| Reference |
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| Authors |
Wildermuth MC, Fall R. |
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| Title |
Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts). |
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| Journal |
Plant. Physiol. 112 (1996) 171-182. |
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| Reference |
4 |
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| Authors |
Schnitzler, J.P., Arenz, R., Steinbrecher, R. and Lehming, A. |
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| Title |
Characterization of an isoprene synthase from leaves of Quercus petraea. |
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| Journal |
Bot. Acta 109 (1996) 216-221. |
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| Reference |
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| Authors |
Miller B, Oschinski C, Zimmer W. |
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| Title |
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli. |
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| Journal |
Planta. 213 (2001) 483-7. |
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| Reference |
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| Authors |
Sivy TL, Shirk MC, Fall R. |
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| Title |
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis. |
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| Journal |
Biochem. Biophys. Res. Commun. 294 (2002) 71-5. |
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| Reference |
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| Authors |
Sasaki K, Ohara K, Yazaki K. |
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| Title |
Gene expression and characterization of isoprene synthase from Populus alba. |
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| Journal |
FEBS. Lett. 579 (2005) 2514-8. |
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| Reference |
|
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| Authors |
Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ. |
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| Title |
Biochemical properties of isoprene synthase in poplar (Populus x canescens). |
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| Journal |
Planta. 222 (2005) 777-86. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 139172-14-8 |
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