KEGG   ENZYME: 4.2.3.42Help
Entry
EC 4.2.3.42                 Enzyme                                 

Name
aphidicolan-16beta-ol synthase;
PbACS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
9alpha-copalyl-diphosphate diphosphate-lyase (aphidicolan-16beta-ol-forming)
Reaction(IUBMB)
9alpha-copalyl diphosphate + H2O = aphidicolan-16beta-ol + diphosphate [RN:R06313]
Reaction(KEGG)
Substrate
9alpha-copalyl diphosphate [CPD:C11902];
H2O [CPD:C00001]
Product
aphidicolan-16beta-ol [CPD:C11890];
diphosphate [CPD:C00013]
Comment
This is a bifunctional enzyme which also has EC 5.5.1.14 syn-copalyl diphosphate synthase activity. Aphidicolan-16beta-ol is a precursor of aphidicolin, a specific inhibitor of DNA polymerase alpha (EC 2.7.7.7).
History
EC 4.2.3.42 created 2009
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K12928  
aphidicolan-16beta-ol synthase/syn-copalyl-diphosphate synthase
Reference
1  [PMID:11457369]
  Authors
Oikawa H, Toyomasu T, Toshima H, Ohashi S, Kawaide H, Kamiya Y, Ohtsuka M, Shinoda S, Mitsuhashi W, Sassa T
  Title
Cloning and functional expression of cDNA encoding aphidicolan-16 beta-ol synthase: a key enzyme responsible for formation of an unusual diterpene skeleton in biosynthesis of aphidicolin.
  Journal
J. Am. Chem. Soc. 123 (2001) 5154-5.
  Sequence
Reference
2  [PMID:14745177]
  Authors
Toyomasu T, Nakaminami K, Toshima H, Mie T, Watanabe K, Ito H, Matsui H, Mitsuhashi W, Sassa T, Oikawa H
  Title
Cloning of a gene cluster responsible for the biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase alpha.
  Journal
Biosci. Biotechnol. Biochem. 68 (2004) 146-52.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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