KEGG   ENZYME: 4.3.1.13Help
Entry
EC 4.3.1.13                 Enzyme                                 

Name
carbamoyl-serine ammonia-lyase;
O-carbamoyl-L-serine deaminase;
carbamoylserine deaminase;
O-carbamoyl-L-serine ammonia-lyase (pyruvate-forming)
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
BRITE hierarchy
Sysname
O-carbamoyl-L-serine ammonia-lyase (decarboxylating; pyruvate-forming)
Reaction(IUBMB)
O-carbamoyl-L-serine + H2O = pyruvate + 2 NH3 + CO2 (overall reaction) [RN:R00213];
(1a) O-carbamoyl-L-serine = CO2 + NH3 + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
O-carbamoyl-L-serine [CPD:C03015];
H2O [CPD:C00001];
2-aminoprop-2-enoate;
2-iminopropanoate
Product
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
CO2 [CPD:C00011];
2-aminoprop-2-enoate;
2-iminopropanoate (spontaneous)
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-oxygen bond, releasing CO2, ammonia, and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and a second ammonia molecule. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase.
History
EC 4.3.1.13 created 1976
Reference
1  [PMID:4761084]
  Authors
Copper AJ, Meister A.
  Title
Enzymatic conversion of O-carbamyl-L-serine to pyruvate and ammonia.
  Journal
Biochem. Biophys. Res. Commun. 55 (1973) 780-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
52227-64-2

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