KEGG   ENZYME: 4.3.1.30Help
Entry
EC 4.3.1.30                 Enzyme                                 

Name
dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase;
desII (gene name);
eryCV (gene name);
MegCV
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
BRITE hierarchy
Sysname
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose ammonia lyase (dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose-forming)
Reaction(IUBMB)
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor = dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor [RN:R08583]
Reaction(KEGG)
Substrate
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose [CPD:C04268];
S-adenosyl-L-methionine [CPD:C00019];
reduced acceptor [CPD:C00030]
Product
dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose [CPD:C11909];
NH3 [CPD:C00014];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
acceptor [CPD:C00028]
Comment
The enzyme, which is a member of the 'AdoMet radical' (radical SAM) family, is involved in biosynthesis of TDP-alpha-D-desosamine. The reaction starts by the transfer of an electron from the reduced form of the enzyme's [4Fe-4S] cluster to S-adenosyl-L-methionine, spliting it into methionine and the radical 5-deoxyadenosin-5'-yl, which attacks the sugar substrate.
History
EC 4.3.1.30 created 2011
Pathway
Polyketide sugar unit biosynthesis
Biosynthesis of antibiotics
Orthology
K13309  
dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase
Genes
STRE: 
STRM: 
SEN: 
SACE_0715(eryCV)
Taxonomy
Reference
1  [PMID:19746907]
  Authors
Szu PH, Ruszczycky MW, Choi SH, Yan F, Liu HW
  Title
Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine.
  Journal
J. Am. Chem. Soc. 131 (2009) 14030-42.
Reference
2  [PMID:20121093]
  Authors
Ruszczycky MW, Choi SH, Liu HW
  Title
Stoichiometry of the redox neutral deamination and oxidative dehydrogenation reactions catalyzed by the radical SAM enzyme DesII.
  Journal
J. Am. Chem. Soc. 132 (2010) 2359-69.
Reference
3  [PMID:21513273]
  Authors
Ruszczycky MW, Choi SH, Mansoorabadi SO, Liu HW
  Title
Mechanistic studies of the radical S-adenosyl-L-methionine enzyme DesII: EPR characterization of a radical intermediate generated during its catalyzed dehydrogenation of TDP-D-quinovose.
  Journal
J. Am. Chem. Soc. 133 (2011) 7292-5.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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