4'-demethylrebeccamycin synthase;
arcyriaflavin A N-glycosyltransferase;
RebG

Lyases;
Carbon-nitrogen lyases;
Amine-lyases

4'-demethylrebeccamycin D-glucose-lyase

4'-O-demethylrebeccamycin + H2O = dichloro-arcyriaflavin A + beta-D-glucose [RN:R09568]

R09568

4'-O-demethylrebeccamycin [CPD:C19700];
H2O [CPD:C00001]

This enzyme catalyses a step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid produced by the Actinobacterium Lechevalieria aerocolonigenes. The enzyme is a glycosylase, and acts in the reverse direction to that shown. It has a wide substrate range, and was shown to glycosylate several substrates, including the staurosporine aglycone, EJG-III-108A, J-104303, 6-N-methyl-arcyriaflavin C and indolo-[2,3-a]-carbazole [1,2].

1  [PMID:10866221]

Ohuchi T, Ikeda-Araki A, Watanabe-Sakamoto A, Kojiri K, Nagashima M, Okanishi M, Suda H

Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccarothrix aerocolonigenes ATCC39243.

J. Antibiot. (Tokyo). 53 (2000) 393-403.

2  [PMID:16575939]

Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS

RebG- and RebM-catalyzed indolocarbazole diversification.

Chembiochem. 7 (2006) 795-804.