KEGG   ENZYME: 4.3.3.5Help
Entry
EC 4.3.3.5                  Enzyme                                 

Name
4'-demethylrebeccamycin synthase;
arcyriaflavin A N-glycosyltransferase;
RebG
Class
Lyases;
Carbon-nitrogen lyases;
Amine-lyases
BRITE hierarchy
Sysname
4'-demethylrebeccamycin D-glucose-lyase
Reaction(IUBMB)
4'-O-demethylrebeccamycin + H2O = dichloro-arcyriaflavin A + beta-D-glucose [RN:R09568]
Reaction(KEGG)
Substrate
4'-O-demethylrebeccamycin [CPD:C19700];
H2O [CPD:C00001]
Product
dichloroarcyriaflavin A [CPD:C19699];
beta-D-glucose [CPD:C00221]
Comment
This enzyme catalyses a step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid produced by the bacterium Lechevalieria aerocolonigenes. The enzyme is a glycosylase, and acts in the reverse direction to that shown. It has a wide substrate range, and was shown to glycosylate several substrates, including the staurosporine aglycone, EJG-III-108A, J-104303, 6-N-methyl-arcyriaflavin C and indolo-[2,3-a]-carbazole [1,2].
History
EC 4.3.3.5 created 2010
Pathway
Staurosporine biosynthesis
Biosynthesis of antibiotics
Orthology
K19888  
4'-demethylrebeccamycin synthase
Reference
1  [PMID:10866221]
  Authors
Ohuchi T, Ikeda-Araki A, Watanabe-Sakamoto A, Kojiri K, Nagashima M, Okanishi M, Suda H
  Title
Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccarothrix aerocolonigenes ATCC39243.
  Journal
J. Antibiot. (Tokyo). 53 (2000) 393-403.
Reference
2  [PMID:16575939]
  Authors
Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS
  Title
RebG- and RebM-catalyzed indolocarbazole diversification.
  Journal
Chembiochem. 7 (2006) 795-804.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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