pyridoxal 5'-phosphate synthase (glutamine hydrolysing);
PdxST

Lyases;
Carbon-nitrogen lyases;
Amine-lyases

D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate (overall reaction) [RN:R10089];
(1a) L-glutamine + H2O = L-glutamate + NH3 [RN:R00256];
(1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 = pyridoxal 5'-phosphate + 4 H2O + phosphate [RN:R10088]

R00256 R10088 R10089;
(other) R07456

D-ribose 5-phosphate [CPD:C00117];
D-glyceraldehyde 3-phosphate [CPD:C00118];
L-glutamine [CPD:C00064];
H2O [CPD:C00001];
NH3 [CPD:C00014]

The ammonia is provided by the glutaminase subunit and channeled to the active site of the lyase subunit by a 100 A tunnel. The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate. The enzyme complex is found in aerobic bacteria, archaea, fungi and plants.

1  [PMID:15771487]

Burns KE, Xiang Y, Kinsland CL, McLafferty FW, Begley TP

Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.

J. Am. Chem. Soc. 127 (2005) 3682-3.

2  [PMID:16030023]

Raschle T, Amrhein N, Fitzpatrick TB

On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis.

J. Biol. Chem. 280 (2005) 32291-300.

3  [PMID:17159152]

Strohmeier M, Raschle T, Mazurkiewicz J, Rippe K, Sinning I, Fitzpatrick TB, Tews I

Structure of a bacterial pyridoxal 5'-phosphate synthase complex.

Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 19284-9.

4  [PMID:17189272]

Raschle T, Arigoni D, Brunisholz R, Rechsteiner H, Amrhein N, Fitzpatrick TB

Reaction mechanism of pyridoxal 5'-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate.

J. Biol. Chem. 282 (2007) 6098-105.

5  [PMID:18260082]

Hanes JW, Keresztes I, Begley TP

Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.

Angew. Chem. Int. Ed. Engl. 47 (2008) 2102-5.

6  [PMID:18271580]

Hanes JW, Burns KE, Hilmey DG, Chatterjee A, Dorrestein PC, Begley TP

Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate.

J. Am. Chem. Soc. 130 (2008) 3043-52.

7  [PMID:18516049]

Hanes JW, Keresztes I, Begley TP

13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase.

Nat. Chem. Biol. 4 (2008) 425-30.

8  [PMID:19152323]

Wallner S, Neuwirth M, Flicker K, Tews I, Macheroux P

Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis.

Biochemistry. 48 (2009) 1928-35.