KEGG   ENZYME: 4.6.1.14Help
Entry
EC 4.6.1.14                 Enzyme                                 

Name
glycosylphosphatidylinositol diacylglycerol-lyase;
(glycosyl)phosphatidylinositol-specific phospholipase C;
GPI-PLC;
GPI-specific phospholipase C;
VSG-lipase;
glycosyl inositol phospholipid anchor-hydrolyzing enzyme;
glycosylphosphatidylinositol-phospholipase C;
glycosylphosphatidylinositol-specific phospholipase C;
variant-surface-glycoprotein phospholipase C;
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase (1,2-cyclic-phosphate-forming)
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase [6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate-forming]
Reaction(IUBMB)
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol = 6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol [RN:R07635]
Reaction(KEGG)
R07635;
(other) R04459
Show
Substrate
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248]
Product
6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate [CPD:C16070];
1,2-diacyl-sn-glycerol [CPD:C00641]
Comment
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphostphatidylinositol (GPI) anchors. In some cases, the long-chain acyl group at the sn-1 position of glycerol is replaced by an alkyl or alk-1-enyl group. In other cases, the diacylglycerol is replaced by ceramide (see Lip-1.4 and Lip-1.5 for definition). The only characterized enzyme with this specificity is from Trypanosoma brucei, where the acyl groups are myristoyl, but the function of the trypanosome enzyme is unknown. Substitution on O-2 of the inositol blocks action of this enzyme. It is not identical with EC 3.1.4.50, glycosylphosphatidylinositol phospholipase D.
History
EC 4.6.1.14 created 1989 as EC 3.1.4.47, transferred 2002 to EC 4.6.1.14
Orthology
K06038  
glycosylphosphatidylinositol diacylglycerol-lyase
Genes
TBR: 
TCR: 
Taxonomy
Reference
1  [PMID:3759991]
  Authors
Hereld D, Krakow JL, Bangs JD, Hart GW, Englund PT.
  Title
A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein.
  Journal
J. Biol. Chem. 261 (1986) 13813-9.
Reference
2  [PMID:9476790]
  Authors
Carnall N, Webb H, Carrington M.
  Title
Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.
  Journal
Mol. Biochem. Parasitol. 90 (1997) 423-32.
Reference
3  [PMID:10764777]
  Authors
Armah DA, Mensa-Wilmot K.
  Title
Tetramerization of glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei.
  Journal
J. Biol. Chem. 275 (2000) 19334-42.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
129070-68-4

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