KEGG   ENZYME: 4.6.1.15Help
Entry
EC 4.6.1.15                 Enzyme                                 

Name
FAD-AMP lyase (cyclizing);
FMN cyclase;
FAD AMP-lyase (cyclic-FMN-forming)
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
FAD AMP-lyase (riboflavin-cyclic-4',5'-phosphate-forming)
Reaction(IUBMB)
FAD = AMP + riboflavin cyclic-4',5'-phosphate [RN:R07636]
Reaction(KEGG)
Substrate
FAD [CPD:C00016]
Product
AMP [CPD:C00020];
riboflavin cyclic-4',5'-phosphate [CPD:C16071]
Comment
Requires Mn2+ or Co2+. While FAD was the best substrate tested [2], the enzyme also splits ribonucleoside diphosphate-X compounds in which X is an acyclic or cyclic monosaccharide or derivative bearing an X-OH group that is able to attack internally the proximal phosphorus with the geometry necessary to form a P=X product; either a five-atom monocyclic phosphodiester or a cis-bicyclic phosphodiester-pyranose fusion. The reaction is strongly inhibited by ADP or ATP but is unaffected by the presence of the product, cFMN.
History
EC 4.6.1.15 created 2002
Reference
1  [PMID:9480905]
  Authors
Fraiz FJ, Pinto RM, Costas MJ, Aavalos M, Canales J, Cabezas A, Cameselle JC.
  Title
Enzymic formation of riboflavin 4',5'-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1.
  Journal
Biochem. J. 330 ( Pt 2) (1998) 881-8.
  Organism
Rattus norvegicus
Reference
2  [PMID:11695920]
  Authors
Cabezas A, Pinto RM, Fraiz F, Canales J, Gonzalez-Santiago S, Cameselle JC.
  Title
Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phosphodiester of X, either a monocyclic compound or a cis-bicyclic phosphodiester-pyranose fusion.
  Journal
Biochemistry. 40 (2001) 13710-22.
  Organism
Rattus norvegicus
  Sequence
[up:Q4KLZ6]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
208349-48-8

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