KEGG   ENZYME: 4.99.1.3Help
Entry
EC 4.99.1.3                 Enzyme                                 

Name
sirohydrochlorin cobaltochelatase;
CbiK;
CbiX;
CbiXS;
anaerobic cobalt chelatase;
cobaltochelatase [ambiguous];
sirohydrochlorin cobalt-lyase (incorrect)
Class
Lyases;
Other lyases;
Sole sub-subclass for lyases that do not belong in the other subclasses
BRITE hierarchy
Sysname
cobalt-sirohydrochlorin cobalt-lyase (sirohydrochlorin-forming)
Reaction(IUBMB)
cobalt-sirohydrochlorin + 2 H+ = sirohydrochlorin + Co2+ [RN:R05807]
Reaction(KEGG)
Substrate
cobalt-sirohydrochlorin [CPD:C11538];
H+ [CPD:C00080]
Product
sirohydrochlorin [CPD:C05778];
Co2+ [CPD:C00175]
Comment
This enzyme is a type II chelatase, being either a monomer (CbiX) or a homodimer (CibK) and being ATP-independent. CbiK from Salmonella enterica uses precorrin-2 as the substrate to yield cobalt-precorrin-2. The enzyme contains two histidines at the active site that are thought to be involved in the deprotonation of the tetrapyrrole substrate as well as in metal binding. CbiX from Bacillus megaterium inserts cobalt at the level of sirohydrochlorin (factor-II) rather than precorrin-2.
History
EC 4.99.1.3 created 2004
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01100  Metabolic pathways
Orthology
K02190  sirohydrochlorin cobaltochelatase
K03795  sirohydrochlorin cobaltochelatase
K22011  sirohydrochlorin cobalto/nickelchelatase
Genes
SEX: STBHUCCB_9050
SENT: TY21A_04315
STM: STM2025(cbiK)
SEO: STM14_2513(cbiK)
SEV: STMMW_20561
SEY: SL1344_2001(cbiK)
SEM: STMDT12_C20470
SEJ: STMUK_2055(cbiK)
SEB: STM474_2110(cbiK)
SEF: UMN798_2190(cbiK)
SENR: STMDT2_19981(cbiK)
SEND: DT104_20831(cbiK)
SENI: CY43_10940
SPT: SPA0846(cbiK)
SEK: SSPA0791
SEI: SPC_1690(cbiK)
SEC: SCH_2033(cbiK)
SHB: SU5_02619
SENS: Q786_09995
SED: SeD_A2360
SEG: SG2050(cbiK)
SEL: SPUL_0875(cbiK)
SEGA: SPUCDC_0875(cbiK)
SET: SEN2023(cbiK)
SENA: AU38_10210
SENO: AU37_10220
SENV: AU39_10220
SENQ: AU40_11465
SENL: IY59_10505
SEEP: I137_03955
SENE: IA1_10105
KPN: KPN_03190(cbiK)
KPU: KP1_4455
KPP: A79E_0920
KPE: KPK_0926(cbiK)
KPR: KPR_1844(cbiK)
KPJ: N559_1051
KPX: PMK1_00671(cbiK)
KPNU: LI86_05185
KPNK: BN49_0918(cbiK)
KVA: Kvar_0880
KOX: KOX_01380
KOE: A225_4740
CKO: CKO_00813
CRO: ROD_21101(cbiK)
CAMA: F384_10335
EBT: EBL_c14860(cbiK)
EBF: D782_1630
YEN: YE2716(cbiK)
YEY: Y11_16011
YEW: CH47_2064(cbiK)
YET: CH48_3167(cbiK)
YEE: YE5303_31471(cbiK)
YAL: AT01_4050(cbiK)
YFR: AW19_735(cbiK)
YIN: CH53_3382(cbiK)
YKR: CH54_927
YRO: CH64_95(cbiK)
PLU: plu2989(cbiK)
PAY: PAU_01611(cbiK)
XBO: XBJ1_0842
XBV: XBW1_1568(cbiK)
XNE: XNC1_1159
XNM: XNC2_1139
MMK: MU9_1005
ETR: ETAE_1994(cbiK)
ETD: ETAF_1803(cbiK)
ETE: ETEE_4050(cbiK)
VSP: VS_2666
SSE: Ssed_2085
MAQ: Maqu_1136
MCA: MCA2299
HHA: Hhal_1350
CSA: Csal_0467
HEL: HELO_1273
TAU: Tola_1671
RSO: RSc2418
RPI: Rpic_2682
REH: H16_A2993(cbiX)
RME: Rmet_2810(cbiX)
CTI: RALTA_A2467(cbiX)
BMA: BMA0669
BPS: BPSL0962
BTE: BTH_I0820
BCN: Bcen_1858
BCJ: BCAL2679
BAM: Bamb_2518
BMU: Bmul_0825
BMJ: BMULJ_02433(cbiX)
BXE: Bxe_A3641
BPH: Bphy_2228
PNU: Pnuc_0340
RFR: Rfer_2113
AAV: Aave_3165
AJS: Ajs_1918
VEI: Veis_4925
DAC: Daci_3536
RTA: Rta_19390
MPT: Mpe_A1945 Mpe_B0439(cbiX) Mpe_B0474(cbiX)
HAR: HEAR2398
MMS: mma_2458
LCH: Lcho_2598
EBA: ebA3869
AZO: azo3377 azo3530(cbiX)
ABU: Abu_1192
SMUL: SMUL_1551(cbiK)
SHAL: SHALO_1515
SULJ: SJPD1_1528
GLO: Glov_3653
PCA: Pcar_0478(cbiK)
DMA: DMR_00730(cbiK) DMR_15530(cbiK)
PPRF: DPRO_3922(cbiKc) DPRO_3927
LIP: LI1067
LIR: LAW_01107
DRT: Dret_0160
DML: Dmul_03740(cbiK1) Dmul_21120(cbiK2)
DAT: HRM2_07680(cbiK1) HRM2_07700(cbiK2) HRM2_45790(cbiK3)
DTO: TOL2_C34190(cbiK) TOL2_C34310(cbiK2)
HOH: Hoch_5277
AVI: Avi_2641
HCI: HCDSEM_005(cbiX)
DSH: Dshi_0171(cbiX2)
MGM: Mmc1_3135
AFR: AFE_3127
BMQ: BMQ_2618(cbiX)
BMD: BMD_2605(cbiX)
GKA: GK1804
GTN: GTNG_1693(cbiX)
AFL: Aflv_2179
LSP: Bsph_3445
LMO: lmo1202(cbiK)
LMF: LMOf2365_1211(cbiK)
LMH: LMHCC_1449(cbiK)
LMC: Lm4b_01206(cbiK)
LMN: LM5578_1275(cbiK)
LMY: LM5923_1228(cbiK)
LMS: LMLG_1058
LMQ: LMM7_1207(cbiK)
LML: lmo4a_1184(cbiK)
LMP: MUO_06200
LMW: LMOSLCC2755_1194(cbiK)
LMX: LMOSLCC2372_1198(cbiK)
LMZ: LMOSLCC2482_1242(cbiK)
LMON: LMOSLCC2376_1153(cbiK)
LMOC: LMOSLCC5850_1191(cbiK)
LMOS: LMOSLCC7179_1169(cbiK)
LMOO: LMOSLCC2378_1207(cbiK)
LMOY: LMOSLCC2479_1199(cbiK)
LMOT: LMOSLCC2540_1181(cbiK)
LMOA: LMOATCC19117_1201(cbiK)
LMOL: LMOL312_1189(cbiK)
LMOG: BN389_12200(cbiK)
LMOE: BN418_1413
LMOB: BN419_1409
LMOD: LMON_1195(cbiK)
LMOW: AX10_14515
LMOX: AX24_03390
LMOQ: LM6179_1509(cbiK)
LMR: LMR479A_1223(cbiK)
LMOK: CQ02_06170
LMOM: IJ09_04325
LIN: cbiK
LWE: lwe1159(cbiK)
LSG: lse_1081(cbiK)
LIV: LIV_1133(cbiK)
BBE: BBR47_12460(cbiX)
PLV: ERIC2_c32330(cbiK)
BTS: Btus_0412
SSA: SSA_0475
LRE: Lreu_1711
LRF: LAR_1599
LRU: HMPREF0538_20898(cbiK)
PCE: PECL_1389(cbiK) PECL_1391(cbiK)
AUR: HMPREF9243_1731(cbiK)
CAC: CA_C1373(cbiK)
CAE: SMB_G0597 SMB_G1396(cbiK)
CPE: CPE1228
CPF: CPF_1436(cbiK)
CPR: CPR_1242(cbiK)
CTC: CTC_00743(cbiK)
CTET: BN906_00779(cbiK)
CNO: NT01CX_0573(cbiK)
CBO: CBO0952(cbiK)
CBA: CLB_0994(cbiK)
CBH: CLC_1008(cbiK)
CBY: CLM_1103(cbiK)
CBL: CLK_0390(cbiK)
CBK: CLL_A2939
CBB: CLD_3608(cbiK)
CBI: CLJ_B1003(cbiK)
CBN: CbC4_2306(cbiK)
CBT: CLH_2685
CBF: CLI_1042(cbiK)
CBM: CBF_1011(cbiK)
CBE: Cbei_2309
CBZ: Cbs_2309
CBEI: LF65_03228
CKL: CKL_0720(cbiK)
CKR: CKR_0642
CLJ: CLJU_c31850(cbiK)
CSR: Cspa_c36280(cbiK)
CPAS: Clopa_1203
CPAT: CLPA_c12520(cbiK)
CPAE: CPAST_c12520(cbiK)
CSB: CLSA_c32520(cbiK)
CSQ: CSCA_5075
CLD: CLSPO_c09510(cbiK)
CTYK: CTK_C09510(cbiK)
AOE: Clos_0323
RUM: CK1_12360
CCT: CC1_20470
ROB: CK5_24160
CPY: Cphy_1387
CSO: CLS_16520
CDF: CD630_34220(cbiK)
PDC: CDIF630_03730(cbiKP)
CDC: CD196_3198(cbiK)
CDL: CDR20291_3244(cbiK)
PDF: CD630DERM_34220(cbiK)
CST: CLOST_0268(cbiK)
SLP: Slip_0928
DRM: Dred_2730
DAE: Dtox_0558
SGY: Sgly_1337
HMO: HM1_0528(cbiK)
ELM: ELI_0774
EHL: EHLA_0208
AWO: Awo_c30260(cbiK)
SAY: TPY_1364(cbiX)
BPRS: CK3_27580
TIT: Thit_0350
TOC: Toce_0309
TTM: Tthe_1847
VRM: 44547418_01200(cbiKp) 44547418_01201(cbiK_1) 44547418_01205(cbiK_2)
MED: MELS_0465
SRI: SELR_08950(cbiK) SELR_17050(cbiK)
MHG: MHY_04300
SCO: SCO1858(SCI39.05)
SMA: SAVERM_6405(cbiX)
TCU: Tcur_1320
SRO: Sros_7366
FAL: FRAAL1021
SAQ: Sare_2699
CAI: Caci_6031
TBI: Tbis_2563
RXY: Rxyl_0646
AFO: Afer_0799
GPA: GPA_01640
CBAC: JI75_02755
SYN: sll0037
SYW: SYNW0837
SYG: sync_1195(cbiX)
SYR: SynRCC307_0990(cbiX)
SYX: SynWH7803_1422(cbiX)
CYA: CYA_2373(cbiX)
CYB: CYB_2347(cbiX)
TEL: tll1398
PMA: Pro_0806
PMT: PMT_0901
AMR: AM1_2009
CYT: cce_4308
TER: Tery_4741
GVI: gll4193
CAU: Caur_2572
CAG: Cagg_1271
HAU: Haur_2923
DGE: Dgeo_0274
AGL: PYTT_2522
MIN: Minf_0288
PSL: Psta_4162
TDE: TDE2656(cbiK)
TPED: TPE_1168(cbiK)
FNU: FN1263
LBA: Lebu_0022
TAI: Taci_0054
SBR: SY1_17900
BFR: BF2511
BVU: BVU_3107
BCEL: BcellWH2_00346(cbiKp) BcellWH2_00350(cbiK_1) BcellWH2_00357(cbiK_2)
PGI: PG_0669(fetB)
PGN: PGN_0705
PGT: PGTDC60_1794(fetB)
TFO: BFO_2214
PSAC: PSM36_1723
PRU: PRU_1084
CPI: Cpin_4473
CTE: CT0389
CPC: Cpar_1479
CLI: Clim_1010
PAA: Paes_1292
PROC: Ptc2401_01277(cbiKp)
CTS: Ctha_2223
TTK: TST_0126(cbiK)
TME: Tmel_0696
TAF: THA_1066(cbiX)
DDF: DEFDS_2060(cbiK)
MJA: MJ_0970
MMP: MMP0164(cbiX(s))
MMD: GYY_00840
MAE: Maeo_1381
MVO: Mvol_1554
MAC: MA_3631(cbiX)
MBAR: MSBR2_1804
MBAK: MSBR3_2859
MMA: MM_0517
MMAC: MSMAC_0768
MTHE: MSTHC_0834
MTHR: MSTHT_2448
MHOR: MSHOH_0892
MBU: Mbur_1035(cbiXS)
MMET: MCMEM_1517
MMH: Mmah_1410
MPY: Mpsy_2755
MTP: Mthe_0104
MCJ: MCON_0747(cbiK) MCON_1928(cbiX) MCON_2896(cbiK)
MBG: BN140_0720(cbiX)
MBN: Mboo_1417
MPL: Mpal_0681
MPD: MCP_0361(cbiX-1) MCP_2528(cbiX-2)
MEZ: Mtc_0061(cbiX)
RCI: RCIX2006(cbiX-1) RCIX98(cbiX-2)
MTH: MTH_1397
MMG: MTBMA_c17830(cbiX)
METC: MTCT_1270
MWO: MWSIV6_1777(cbiX)
METE: tca_01350(cbiX)
MST: Msp_1561 Msp_1563(cbiX)
MRU: mru_0895(cbiK) mru_2200(cbiX)
MEB: Abm4_0618(cbiX1) Abm4_0619(cbiX2)
MMIL: sm9_2307(cbiX1) sm9_2308(cbiX2)
METH: MBMB1_1943
MFC: BRM9_0750(cbiX) BRM9_2283
MCUB: MCBB_2224(cbiX)
MFV: Mfer_0962
MKA: MK0428
AFU: AF_0721
FPL: Ferp_0608
HAL: VNG_1093C
HSL: OE_2585R(cbiX2)
HMA: rrnAC1289
NPH: NP_1588A(cbiX2)
HUT: Huta_1760
HMU: Hmuk_2179
HWA: HQ_1656A(cbiX2)
HVO: HVO_1128(cbiX2)
HLA: Hlac_0262
HTU: Htur_1053
NMG: Nmag_3212
MEAR: Mpt1_c07010(cbiX1) Mpt1_c08040(cbiX2) Mpt1_c08050(cbiX3)
IHO: Igni_0537
SSO: SSO0410
SOL: Ssol_1387
SSOA: SULA_1433
SSOL: SULB_1434
SSOF: SULC_1432
STO: STK_03830(cbiXS)
SAI: Saci_0830
SID: M164_1741
SII: LD85_1951
SIH: SiH_1669
SIR: SiRe_1590
SIC: SiL_1582
MSE: Msed_2245
MCN: Mcup_0064
AHO: Ahos_0715
 » show all
Taxonomy
Reference
1  [PMID:10451360]
  Authors
Schubert HL, Raux E, Wilson KS, Warren MJ.
  Title
Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis.
  Journal
Biochemistry. 38 (1999) 10660-9.
  Sequence
[stm:STM2025]
Reference
2  [PMID:12686546]
  Authors
Brindley AA, Raux E, Leech HK, Schubert HL, Warren MJ.
  Title
A story of chelatase evolution: identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaea.
  Journal
J. Biol. Chem. 278 (2003) 22388-95.
  Sequence
[mba:Mbar_A0344] [mth:MTH_1397]
Reference
3  [PMID:12195810]
  Authors
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC.
  Title
The biosynthesis of adenosylcobalamin (vitamin B12).
  Journal
Nat. Prod. Rep. 19 (2002) 390-412.
Other DBs
ExplorEnz - The Enzyme Database: 4.99.1.3
IUBMB Enzyme Nomenclature: 4.99.1.3
ExPASy - ENZYME nomenclature database: 4.99.1.3
BRENDA, the Enzyme Database: 4.99.1.3

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