KEGG   ENZYME: 4.99.1.5Help
Entry
EC 4.99.1.5                 Enzyme                                 

Name
aliphatic aldoxime dehydratase;
OxdA;
aliphatic aldoxime hydro-lyase
Class
Lyases;
Other lyases;
Sole sub-subclass for lyases that do not belong in the other subclasses
BRITE hierarchy
Sysname
aliphatic aldoxime hydro-lyase (aliphatic-nitrile-forming)
Reaction(IUBMB)
an aliphatic aldoxime = an aliphatic nitrile + H2O [RN:R02827]
Reaction(KEGG)
Substrate
aliphatic aldoxime [CPD:C02658]
Product
aliphatic nitrile [CPD:C16072];
H2O [CPD:C00001]
Comment
The enzyme from Pseudomonas chlororaphis contains Ca2+ and protoheme IX, the iron of which must be in the form Fe(II) for activity. The enzyme exhibits a strong preference for aliphatic aldoximes, such as butyraldoxime and acetaldoxime, over aromatic aldoximes, such as pyridine-2-aldoxime, which is a poor substrate. No activity was found with the aromatic aldoximes benzaldoxime and pyridine-4-aldoxime.
History
EC 4.99.1.5 created 2004
Orthology
K13028  
aldoxime dehydratase
Genes
SRY: 
EBI: 
PVA: 
PAO: 
PPF: 
PPB: 
PPI: 
PSB: 
PFS: 
PFW: 
PFF: 
PPUU: 
PSK: 
PSW: 
PPV: 
ACI: 
SWD: 
HAA: 
RPJ: 
BCN: 
BCH: 
BCM: 
BAM: 
BAC: 
BCON: 
BGD: 
BHZ: 
AAA: 
VAP: 
VPE: 
VPD: 
HYR: 
HSE: 
HSZ: 
HRB: 
SFH: 
BJU: 
BJP: 
BBT: 
BRS: 
BRC: 
BRAD: 
BIC: 
XAU: 
HMC: 
HYPMC_0479(Adase)
SWI: 
SPHR: 
GOH: 
RHA: 
RER: 
REY: 
REB: 
ROA: 
SALB: 
ARR: 
AAU: 
AAur_1899(oxdA)
PSIM: 
PSEE: 
PSEH: 
PSEQ: 
PECQ: 
AMI: 
 » show all
Taxonomy
Reference
1  [PMID:12773527]
  Authors
Oinuma K, Hashimoto Y, Konishi K, Goda M, Noguchi T, Higashibata H, Kobayashi M.
  Title
Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23. Sequencing, gene expression, purification, and characterization.
  Journal
J. Biol. Chem. 278 (2003) 29600-8.
  Sequence
Reference
2  [PMID:14556637]
  Authors
Xie SX, Kato Y, Komeda H, Yoshida S, Asano Y.
  Title
A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4.
  Journal
Biochemistry. 42 (2003) 12056-66.
  Sequence
Reference
3  [PMID:16233624]
  Authors
Kato Y, Yoshida S, Xie SX, Asano Y.
  Title
Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771.
  Journal
J. Biosci. Bioeng. 97 (2004) 250-9.
  Sequence
[rer:RER_59190]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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