KEGG   ENZYME: 5.1.3.30Help
Entry
EC 5.1.3.30                 Enzyme                                 

Name
D-psicose 3-epimerase;
DPEase (ambiguous)
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
D-psicose 3-epimerase
Reaction(IUBMB)
D-psicose = D-fructose [RN:R10817]
Reaction(KEGG)
Substrate
D-psicose [CPD:C06468]
Product
D-fructose [CPD:C00095]
Comment
The enzyme is highly specific for D-psicose and shows very low activity with D-tagatose (cf. EC 5.1.3.31, D-tagatose 3-epimerase). The enzyme from the bacterium Clostridium scindens requires Mn2+ [1], whereas the enzymes from the bacteria Clostridium cellulolyticum [2,5], Clostridium sp. BNL1100 [3], and Clostridium bolteae [4] require Co2+ as optimum cofactor. The enzyme from Ruminococcus sp. [6] is not dependent on the presence of metal ions, but its activity is enhanced by Mn2+.
History
EC 5.1.3.30 created 2014
Orthology
K18910  
D-psicose/D-tagatose/L-ribulose 3-epimerase
Genes
SFO: 
VNI: 
MMT: 
BCEN: 
BMU: 
BMK: 
BGP: 
BGU: 
BGD: 
BGO: 
BYI: 
BUO: 
BUE: 
BPLA: 
BCAI: 
VEI: 
DAC: 
DEL: 
LIM: 
MLO: 
MCI: 
MOP: 
MAM: 
HOE: 
SME: 
SMK: 
SMQ: 
SMX: 
SMI: 
SMEG: 
SMEL: 
SMER: 
SMD: 
RHI: 
SFH: 
SFD: 
EAD: 
ATU: 
ARA: 
ATA: 
RET: 
REL: 
REI: 
RLE: 
RLT: 
RLG: 
RLB: 
RLU: 
RTR: 
RIR: 
RHL: 
RGA: 
NGL: 
NGG: 
SHZ: 
CHEL: 
DEQ: 
MEY: 
MAAD: 
AUA: 
RSP: 
RSH: 
RSQ: 
RSK: 
RDE: 
RLI: 
DSH: 
MALG: 
PPHR: 
HAT: 
ABG: 
ABS: 
ABQ: 
SPSY: 
CLS: 
CLB: 
CCE: 
ROB: 
BPRL: 
SCB: 
SBH: 
SDV: 
SALB: 
STRC: 
MIM: 
MIP: 
ARR: 
AAU: 
LMOI: 
IVA: 
MPH: 
NDA: 
NAL: 
NML: 
MMAR: 
SVI: 
KPHY: 
VMA: 
SNA: 
SYZ: 
SYY: 
SYNGTS_1069(sll1304)
SYT: 
SYNGTI_1069(sll1304)
SYS: 
SYNPCCN_1068(sll1304)
SYQ: 
SYNPCCP_1068(sll1304)
SYJ: 
SYNE: 
SYNP: 
GEN: 
CAP: 
MSV: 
CAA: 
SBU: 
SGP: 
STR: 
RBA: 
PSL: 
PLM: 
PBS: 
IPA: 
SACI: 
HHY: 
PCM: 
SHT: 
CMR: 
CAMU: 
ALM: 
DFE: 
SLI: 
SRD: 
RSI: 
EOL: 
FAE: 
FBT: 
FJO: 
FBC: 
ZGA: 
CIH: 
HJE: 
 » show all
Taxonomy
Reference
1  [PMID:21663329]
  Authors
Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B
  Title
Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10.
  Journal
J. Agric. Food. Chem. 59 (2011) 7785-92.
  Sequence
[cce:Ccel_0941]
Reference
2  [PMID:22426981]
  Authors
Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Ma Y, Guo RT, Sun Y
  Title
Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars.
  Journal
Protein. Cell. 3 (2012) 123-31.
Reference
3  [PMID:22760176]
  Authors
Zhu Y, Men Y, Bai W, Li X, Zhang L, Sun Y, Ma Y
  Title
Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production.
  Journal
Biotechnol. Lett. 34 (2012) 1901-6.
Reference
4  [PMID:23646168]
  Authors
Zhang W, Fang D, Xing Q, Zhou L, Jiang B, Mu W
  Title
Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704.
  Journal
PLoS. One. 8 (2013) e62987.
Reference
5  [PMID:23660703]
  Authors
Mu W, Zhang W, Fang D, Zhou L, Jiang B, Zhang T
  Title
Characterization of a D-psicose-producing enzyme, D-psicose 3-epimerase, from Clostridium sp.
  Journal
Biotechnol. Lett. 35 (2013) 1481-6.
  Sequence
Reference
6  [PMID:23644747]
  Authors
Jia M, Mu W, Chu F, Zhang X, Jiang B, Zhou LL, Zhang T
  Title
A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production: cloning, expression, purification, and characterization.
  Journal
Appl. Microbiol. Biotechnol. 98 (2014) 717-25.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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