KEGG   ENZYME: 5.5.1.15Help
Entry
EC 5.5.1.15                 Enzyme                                 

Name
terpentedienyl-diphosphate synthase;
terpentedienol diphosphate synthase;
Cyc1;
clerodadienyl diphosphate synthase;
terpentedienyl-diphosphate lyase (decyclizing)
Class
Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
terpentedienyl-diphosphate lyase (ring-opening)
Reaction(IUBMB)
geranylgeranyl diphosphate = terpentedienyl diphosphate [RN:R09714]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:C00353]
Product
terpentedienyl diphosphate [CPD:C19815]
Comment
Requires Mg2+. Contains a DXDD motif, which is a characteristic of diterpene cylases whose reactions are initiated by protonation at the 14,15-double bond of geranylgeranyl diphosphate (GGDP) [2]. The triggering proton is lost at the end of the cyclization reaction [3]. The product of the reaction, terpentedienyl diphosphate, is the substrate for EC 4.2.3.36, terpentetriene synthase and is a precursor of the diterpenoid antibiotic terpentecin.
History
EC 5.5.1.15 created 2008
Pathway
Biosynthesis of antibiotics
Orthology
K19835  
terpentedienyl-diphosphate synthase
Reference
1  [PMID:11567009]
  Authors
Dairi T, Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H.
  Title
Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin.
  Journal
J. Bacteriol. 183 (2001) 6085-94.
  Sequence
Reference
2  [PMID:12138123]
  Authors
Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H, Dairi T.
  Title
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin.
  Journal
J. Biol. Chem. 277 (2002) 37098-104.
  Sequence
Reference
3  [PMID:12839434]
  Authors
Eguchi T, Dekishima Y, Hamano Y, Dairi T, Seto H, Kakinuma K.
  Title
A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase.
  Journal
J. Org. Chem. 68 (2003) 5433-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system