KEGG   ENZYME: 6.1.1.27Help
Entry
EC 6.1.1.27                 Enzyme                                 

Name
O-phospho-L-serine---tRNA ligase;
O-phosphoseryl-tRNA ligase;
non-canonical O-phosphoseryl-tRNA synthetase;
SepRS
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy
Sysname
O-phospho-L-serine:tRNACys ligase (AMP-forming)
Reaction(IUBMB)
ATP + O-phospho-L-serine + tRNACys = AMP + diphosphate + O-phospho-L-seryl-tRNACys [RN:R08576]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
O-phospho-L-serine [CPD:C01005];
tRNA(Cys) [CPD:C01639]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
O-phospho-L-seryl-tRNACys
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) [2].
History
EC 6.1.1.27 created 2009
Pathway
Aminoacyl-tRNA biosynthesis
Orthology
K07587  
O-phosphoseryl-tRNA synthetase
Genes
MJA: 
MFE: 
MVU: 
MFS: 
MIF: 
MIG: 
MMP: 
MMQ: 
MMX: 
MMZ: 
MMD: 
MAE: 
MVN: 
MVO: 
MOK: 
MAC: 
MA0090(pheRS)
MBA: 
MMA: 
MMAZ: 
MBU: 
MMH: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MCON_3135(sepS)
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MEZ: 
RCI: 
MTH: 
MMG: 
MEL: 
MEW: 
METH: 
MFV: 
MKA: 
AFU: 
AFG: 
APO: 
AVE: 
AST: 
FPL: 
 » show all
Taxonomy
Reference
1  [PMID:17351629]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
  Journal
Nat. Struct. Mol. Biol. 14 (2007) 272-9.
  Sequence
[mja:MJ_1660] [afu:AF0110]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J. Biol. Chem. 283 (2008) 22007-17.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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