KEGG   ENZYME: 6.1.2.1Help
Entry
EC 6.1.2.1                  Enzyme                                 

Name
D-alanine---(R)-lactate ligase;
VanA;
VanB;
VanD
Class
Ligases;
Forming carbon-oxygen bonds;
acid-alcohol ligases (ester synthases)
BRITE hierarchy
Sysname
D-alanine:(R)-lactate ligase (ADP-forming)
Reaction(IUBMB)
D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate [RN:R09588]
Reaction(KEGG)
Substrate
D-alanine [CPD:C00133];
(R)-lactate [CPD:C00256];
ATP [CPD:C00002]
Product
D-alanyl-(R)-lactate [CPD:C19694];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
The product of this enzyme, the depsipeptide D-alanyl-(R)-lactate, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4, D-alanine---D-alanine ligase. The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.
History
EC 6.1.2.1 created 2010
Orthology
K15739  
D-alanine---(R)-lactate ligase
Genes
BBE: 
PLV: 
EFA: 
EF2294(vanB)
EFC: 
EFAU: 
ECAS: 
CLB: 
AOE: 
RBR: 
CLE: 
CSO: 
CAD: 
CDF: 
CDC: 
CDL: 
CDG: 
DSY: 
DSY3690(vanB)
DHD: 
Dhaf_1664(vanB)
DDL: 
DGI: 
OVA: 
ERS: 
EUC: 
TPR: 
SCO: 
SCO3595(vanB)
SLV: 
KFL: 
FRE: 
AMD: 
AMED_8289(ddlA)
AMN: 
RAM_42565(vanB)
AMM: 
AMES_8162(ddlA)
AMZ: 
B737_8163(ddlA)
AOI: 
AJA: 
SESP: 
KAL: 
CAI: 
Caci_5793(vanB)
SNA: 
CWO: 
OLS: 
 » show all
Taxonomy
Reference
1  [PMID:1931965]
  Authors
Bugg TD, Wright GD, Dutka-Malen S, Arthur M, Courvalin P, Walsh CT
  Title
Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.
  Journal
Biochemistry. 30 (1991) 10408-15.
  Sequence
[up:P25051]
Reference
2  [PMID:7957913]
  Authors
Meziane-Cherif D, Badet-Denisot MA, Evers S, Courvalin P, Badet B
  Title
Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583.
  Journal
FEBS. Lett. 354 (1994) 140-2.
  Sequence
[efa:EF2294]
Reference
3  [PMID:9303405]
  Authors
Perichon B, Reynolds P, Courvalin P
  Title
VanD-type glycopeptide-resistant Enterococcus faecium BM4339.
  Journal
Antimicrob. Agents. Chemother. 41 (1997) 2016-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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