KEGG   ENZYME: 6.2.1.35Help
Entry
EC 6.2.1.35                 Enzyme                                 

Name
ACP-SH:acetate ligase;
HS-acyl-carrier protein:acetate ligase;
[acyl-carrier protein]:acetate ligase;
MadH
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
acetate:[acyl-carrier-protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + acetate + an [acyl-carrier protein] = AMP + diphosphate + an acetyl-[acyl-carrier protein] [RN:R09475]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
acetate [CPD:C00033];
[acyl-carrier protein] [CPD:C00229]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
acetyl-[acyl-carrier protein] [CPD:C03939]
Comment
This enzyme, from the anaerobic bacterium Malonomonas rubra, is a component of the multienzyme complex EC 4.1.1.89, biotin-dependent malonate decarboxylase. The enzyme uses the energy from hydrolysis of ATP to convert the thiol group of the acyl-carrier-protein-bound 2'-(5-phosphoribosyl)-3'-dephospho-CoA prosthetic group into its acetyl thioester [2].
History
EC 6.2.1.35 created 2008
Reference
1  [PMID:1628643]
  Authors
Hilbi H, Dehning I, Schink B, Dimroth P
  Title
Malonate decarboxylase of Malonomonas rubra, a novel type of biotin-containing acetyl enzyme.
  Journal
Eur. J. Biochem. 207 (1992) 117-23.
Reference
2  [PMID:8664258]
  Authors
Berg M, Hilbi H, Dimroth P
  Title
The acyl carrier protein of malonate decarboxylase of Malonomonas rubra contains 2'-(5"-phosphoribosyl)-3'-dephosphocoenzyme A as a prosthetic group.
  Journal
Biochemistry. 35 (1996) 4689-96.
  Organism
Malonomonas rubra
  Sequence
[up:O06928]
Reference
3  [PMID:9128730]
  Authors
Berg M, Hilbi H, Dimroth P
  Title
Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
  Journal
Eur. J. Biochem. 245 (1997) 103-15.
  Organism
Malonomonas rubra
  Sequence
[up:O06928]
Reference
4  [PMID:11902724]
  Authors
Dimroth P, Hilbi H
  Title
Enzymic and genetic basis for bacterial growth on malonate.
  Journal
Mol. Microbiol. 25 (1997) 3-10.
  Organism
Malonomonas rubra
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system