KEGG   ENZYME: 6.2.1.39Help
Entry
EC 6.2.1.39                 Enzyme                                 

Name
[butirosin acyl-carrier protein]---L-glutamate ligase;
[BtrI acyl-carrier protein]---L-glutamate ligase;
BtrJ
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Reaction(IUBMB)
(1) ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] [RN:R08905];
(2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] [RN:R08906]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-glutamate [CPD:C00025];
BtrI acyl-carrier protein;
4-amino butanoyl-[BtrI acyl-carrier protein]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
L-glutamyl-[BtrI acyl-carrier protein];
4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Comment
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
History
EC 6.2.1.39 created 2012
Pathway
Butirosin and neomycin biosynthesis
Biosynthesis of secondary metabolites
Orthology
K13559  
[BtrI acyl-carrier protein]--L-glutamate ligase
Reference
1  [PMID:15975512]
  Authors
Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB
  Title
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.
  Journal
Chem. Biol. 12 (2005) 665-75.
  Organism
Bacillus circulans
  Sequence
[up:Q4H4E7]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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