KEGG   ENZYME: 6.2.1.41Help
Entry
EC 6.2.1.41                 Enzyme                                 

Name
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate---CoA ligase;
fadD3 (gene name);
HIP---CoA ligase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA = AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [RN:R10769]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [CPD:C20838]
Comment
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol and cholate. The enzyme is very specific for its substrate, and requires that the side chain at C17 is completely removed.
History
EC 6.2.1.41 created 2014
Pathway
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K18687  
HIP---CoA ligase
Genes
PRE: 
PPUT: 
PPUN: 
PPUU: 
PKC: 
PFZ: 
PPSY: 
SPL: 
SHL: 
PHA: 
PTN: 
ZAL: 
SDF: 
GPB: 
RIN: 
REU: 
REH: 
H16_B0644(h16_B0644)
CNC: 
CTI: 
CBW: 
BUR: 
BCJ: 
BCEN: 
BCEO: 
BCT: 
BCED: 
BPYR: 
BCON: 
BFN: 
PVE: 
CTT: 
CTES: 
THU: 
AAK: 
NAR: 
NPP: 
NPN: 
SWI: 
SPHM: 
SSAN: 
SCH: 
SYB: 
MTU: 
Rv3561(fadD3)
MTV: 
MTC: 
MRA: 
MRA_3600(fadD3)
MTF: 
MTB: 
MTK: 
MTZ: 
MTG: 
MTI: 
MTE: 
MTUR: 
CFBS_3777(fadD3)
MTL: 
MTO: 
MTD: 
UDA_3561(fadD3)
MTN: 
MTJ: 
MTUB: 
MTUE: 
MTX: 
MTUH: 
MTUL: 
MTUT: 
HKBT1_3762(fadD3)
MTUU: 
HKBT2_3771(fadD3)
MTQ: 
HKBS1_3774(fadD3)
MBO: 
Mb3591(fadD3)
MBB: 
BCG_3625(fadD3)
MBT: 
JTY_3626(fadD3)
MBM: 
MBK: 
MBX: 
MBZ: 
MAF: 
MAF_35730(fadD3)
MCE: 
MCAN_35721(fadD3)
MCQ: 
MCV: 
MCX: 
MCZ: 
MMIC: 
MPA: 
MAP_0506c(fadD3) MAP_0780(fadD3)
MAO: 
MAVI: 
MAVU: 
MAV: 
MAVD: 
MAVR: 
MAVA: 
MIT: 
MIR: 
MIA: 
MIE: 
MID: 
MYO: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MUL: 
MUL_4126(fadD3)
MVA: 
MGI: 
MSP: 
MAB: 
MABB: 
MMV: 
MAY: 
MABO: 
MABL: 
MAZ: 
MAK: 
MYS: 
MYC: 
MMC: 
MKM: 
MJL: 
MJD: 
JDM601_3424(fadD3_2) JDM601_3717(fadD3)
MMI: 
MMAR_4688(fadD3_2) MMAR_5050(fadD3)
MRH: 
MMM: 
MCB: 
MLI: 
MULP_05308(fadD3)
MKN: 
MKS: 
MKI: 
MNE: 
MYV: 
MYE: 
MGO: 
MFT: 
MHAD: 
MPHL: 
MVQ: 
ASD: 
NFA: 
NFR: 
NCY: 
NBR: 
NNO: 
RHA: 
RER: 
REY: 
REB: 
ROP: 
ROA: 
REQ: 
RPY: 
RAV: 
GBR: 
GPO: 
GOR: 
KTR9_3925(fadD3)
GOQ: 
GTA: 
TPR: 
SMA: 
SAV_3806(fadD16)
SGR: 
SGB: 
SCB: 
SVL: 
SCT: 
SCY: 
SBH: 
SVE: 
SDV: 
BN159_5012(fadD16)
SFI: 
SCI: 
SRC: 
SALU: 
SALL: 
SVT: 
STRE: 
SLD: 
STRM: 
SPRI: 
SCZ: 
SCX: 
SRW: 
TUE45_04305(lcfB_5)
ARL: 
ARY: 
NCA: 
NDK: 
PSIM: 
TCU: 
SRO: 
FRE: 
FRI: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
PSEE: 
PSEH: 
PSEQ: 
PECQ: 
PHH: 
KAL: 
KPHY: 
LED: 
ALL: 
STP: 
SAQ: 
AMS: 
CAI: 
CWO: 
ABAI: 
 » show all
Taxonomy
Reference
1  [PMID:16891113]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kudo T
  Title
ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids.
  Journal
J. Steroid. Biochem. Mol. Biol. 101 (2006) 78-84.
  Sequence
Reference
2  [PMID:23146019]
  Authors
Casabon I, Crowe AM, Liu J, Eltis LD
  Title
FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C  and D in actinobacteria.
  Journal
Mol. Microbiol. 87 (2013) 269-83.
  Sequence
[mtu:Rv3561]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system