KEGG   ENZYME: 6.2.1.43Help
Entry
EC 6.2.1.43                 Enzyme                                 

Name
2-hydroxy-7-methoxy-5-methyl-1-naphthoate---CoA ligase;
NcsB2
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
2-hydroxy-7-methoxy-5-methyl-1-naphthoate:CoA ligase
Reaction(IUBMB)
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA = AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA [RN:R10771]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
2-hydroxy-7-methoxy-5-methyl-1-naphthoate [CPD:C20841];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA [CPD:C20842]
Comment
The enzyme from the bacterium Streptomyces carzinostaticus is involved in the attachment of the 2-hydroxy-7-methoxy-5-methyl-1-naphthoate moiety of the antibiotic neocarzinostatin. In vitro the enzyme also catalyses the activation of other 1-naphthoic acid analogues, e.g. 2-hydroxy-5-methyl-1-naphthoate or 2,7-dihydroxy-5-methyl-1-naphthoate.
History
EC 6.2.1.43 created 2014
Orthology
K20423  
2-hydroxy-7-methoxy-5-methyl-1-naphthoate---CoA ligase
Genes
MTS: 
APN: 
PSUL: 
AOI: 
AORI_6598(dhbE)
AMQ: 
PDX: 
SAQ: 
VMA: 
ACTN: 
Taxonomy
Reference
1  [PMID:17539640]
  Authors
Cooke HA, Zhang J, Griffin MA, Nonaka K, Van Lanen SG, Shen B, Bruner SD
  Title
Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
  Journal
J. Am. Chem. Soc. 129 (2007) 7728-9.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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