KEGG   ENZYME: 6.2.1.7Help
Entry
EC 6.2.1.7                  Enzyme                                 
Name
cholate---CoA ligase;
BAL;
bile acid CoA ligase;
bile acid coenzyme A ligase;
choloyl-CoA synthetase;
choloyl coenzyme A synthetase;
cholic thiokinase;
cholate thiokinase;
cholic acid:CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase;
THCA-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate---CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming);
cholyl-CoA synthetase;
trihydroxycoprostanoyl-CoA synthetase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
cholate:CoA ligase (AMP-forming)
Reaction(IUBMB)
(1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA [RN:R02794];
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA [RN:R04580]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
cholate [CPD:C00695];
CoA [CPD:C00010];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
choloyl-CoA [CPD:C01794];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA
Comment
Requires Mg2+ for activity. The mammalian enzyme is membrane-bound and catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [7]. The bacterial enzyme is soluble and participates in an anaerobic bile acid 7 alpha-dehydroxylation pathway [5].
Pathway
Primary bile acid biosynthesis
Metabolic pathways
Orthology
K08748  
solute carrier family 27 (fatty acid transporter), member 5
Genes
HSA: 
10998(SLC27A5)
PTR: 
456351(SLC27A5)
PPS: 
100973518(SLC27A5)
GGO: 
101141402(SLC27A5)
PON: 
MCC: 
MMU: 
26459(Slc27a5)
RNO: 
79111(Slc27a5)
CFA: 
484223(SLC27A5) 608675(SLC27A5)
AML: 
100478693(SLC27A5)
FCA: 
101086686(SLC27A5)
BTA: 
533016(SLC27A5)
SSC: 
100620603
ECB: 
100051678(SLC27A5)
MDO: 
100029941
SHR: 
100916639
OAA: 
100090562
 » show all
Taxonomy
Reference
1  [PMID:13303991]
  Authors
ELLIOTT WH.
  Title
The enzymic activation of cholic acid by guinea-pig-liver microsomes.
  Journal
Biochem. J. 62 (1956) 427-33.
  Organism
Cavia porcellus
Reference
2  [PMID:13403911]
  Authors
ELLIOTT WH.
  Title
The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes.
  Journal
Biochem. J. 65 (1957) 315-21.
  Organism
Cavia porcellus
Reference
3  [PMID:3183523]
  Authors
Prydz K, Kase BF, Bjorkhem I, Pedersen JI.
  Title
Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver.
  Journal
J. Lipid. Res. 29 (1988) 997-1004.
  Organism
Rattus norvegicus [GN:rno]
Reference
4  [PMID:2521999]
  Authors
Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP.
  Title
Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver.
  Journal
Biochem. J. 257 (1989) 221-9.
  Organism
Rattus norvegicus [GN:rno]
Reference
5  [PMID:1551828]
  Authors
Mallonee DH, Adams JL, Hylemon PB
  Title
The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase.
  Journal
J. Bacteriol. 174 (1992) 2065-71.
Reference
6  [PMID:9390170]
  Authors
Wheeler JB, Shaw DR, Barnes S.
  Title
Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.
  Journal
Arch. Biochem. Biophys. 348 (1997) 15-24.
  Organism
Rattus norvegicus [GN:rno]
  Sequence
RNO: 79111
Reference
7  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J. Lipid. Res. 43 (2002) 2062-71.
  Organism
Rattus norvegicus [GN:rno]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9027-90-1
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