KEGG   ENZYME: 6.3.2.25Help
Entry
EC 6.3.2.25                 Enzyme                                 
Name
tubulin---tyrosine ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
alpha-tubulin:L-tyrosine ligase (ADP-forming)
Reaction(IUBMB)
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate [RN:R04730]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
detyrosinated alpha-tubulin [CPD:C05234];
L-tyrosine [CPD:C00082]
Product
alpha-tubulin [CPD:C01392];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
Orthology
K06047  
tubulin---tyrosine ligase
Genes
HSA: 
150465(TTL)
PTR: 
470483(TTL)
PPS: 
GGO: 
PON: 
MCC: 
699075(TTL)
MMU: 
69737(Ttl)
RNO: 
171572(Ttl)
CFA: 
483065(TTL)
FCA: 
BTA: 
514559(TTL)
SSC: 
445530(TTL)
ECB: 
100052052
MDO: 
100031152(TTL)
SHR: 
100930667(TTL)
OAA: 
100084789 100089481
GGA: 
416711(TTL)
MGP: 
100540631
TGU: 
100218069(TTL)
ACS: 
100561131(ttl)
XTR: 
100135000(ttl)
DRE: 
767687(wu:fb93a09)
TRU: 
101075885
OLA: 
101168672
BFO: 
BRAFLDRAFT_65827
SPU: 
582881
NVE: 
NEMVE_v1g99329
TAD: 
TRIADDRAFT_52733
MBR: 
MONBRDRAFT_14687
TET: 
TTHERM_00295800
PTM: 
GSPATT00017619001
TBR: 
Tb927.2.5250
TCR: 
507801.50
LIF: 
LINJ_27_2440
 » show all
Taxonomy
Reference
1  [PMID:3821560]
  Authors
Wehland J, Schroder HC, Weber K.
  Title
Isolation and purification of tubulin tyrosine ligase.
  Journal
Methods. Enzymol. 134 (1986) 170-9.
  Organism
Sus scofa [GN:ssc]
Reference
2  [PMID:7510228]
  Authors
Rudiger M, Wehland J, Weber K.
  Title
The carboxy-terminal peptide of detyrosinated alpha tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase.
  Journal
Eur. J. Biochem. 220 (1994) 309-20.
  Organism
Sus scofa [GN:ssc]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
60321-03-1
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