KEGG   ENZYME: 6.3.2.30Help
Entry
EC 6.3.2.30                 Enzyme                                 

Name
cyanophycin synthase (L-arginine-adding);
CphA (ambiguous);
CphA1 (ambiguous);
CphA2 (ambiguous);
cyanophycin synthetase (ambiguous);
multi-L-arginyl-poly-L-aspartate synthase (ambiguous)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
cyanophycin:L-arginine ligase (ADP-forming)
Reaction(IUBMB)
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 [RN:R09593]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[L-Asp(4-L-Arg)]n-L-Asp [CPD:C19710];
L-Arg [CPD:C00062]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[L-Asp(4-L-Arg)]n+1 [CPD:C19713]
Comment
Requires Mg2+ for activity. Both this enzyme and EC 6.3.2.29, cyanophycin synthase (L-aspartate-adding), are required for the elongation of cyanophycin, which is a protein-like cell inclusion that is unique to cyanobacteria and acts as a temporary nitrogen store [2]. Both enzymes are found in the same protein but have different active sites [2,4]. Both L-Asp and L-Arg must be present before either enzyme will display significant activity [2]. Canavanine and lysine can be incoporated into the polymer instead of arginine [2].
History
EC 6.3.2.30 created 2007
Orthology
K03802  
cyanophycin synthetase
Genes
PSD: 
ACI: 
CPS: 
GNI: 
GNIT_3034(cphA)
GPS: 
C427_4660(cphA)
LLO: 
LLO_2537(cphA)
FTU: 
FTT_1130c(cphA)
FTF: 
FTF1130c(cphA)
FTW: 
FTW_1163(cphA)
FTR: 
FTT: 
FTV_1080(cphA)
FTG: 
FTU_1164(cphA)
FTL: 
FTH: 
FTH_0821(cphA)
FTA: 
FTS: 
FTI: 
FTS_0821(cphA)
FTO: 
FTM: 
FTM_1298(cphA)
FTN: 
FTN_1112(cphA)
FCF: 
FCN: 
FPH: 
FRT: 
FNA: 
FNL: 
MEC: 
TVI: 
CSA: 
GPB: 
LHK: 
RPI: 
RPF: 
REU: 
REH: 
H16_A0774(cphA) H16_A0775(cphA')
CNC: 
CNE_1c07850(cphA1) CNE_1c07860(cphA2)
RME: 
Rmet_0703(cphA1) Rmet_0704(cphA2)
CTI: 
PNU: 
PPK: 
PPNO: 
PRB: 
BPE: 
BP1739(cphA) BP1740(cphA)
BPC: 
BPTD_1717(cphA) BPTD_1718(cphA)
BPER: 
BPA: 
BPP3184(cphA)
BPAR: 
BBR: 
BB3584(cphA) BB3585(cphA)
BBM: 
BBH: 
BPT: 
Bpet1860(cphA1) Bpet1861(cphA2)
BAV: 
BAV1232(cphA1) BAV1233(cphA2)
BHO: 
AXY: 
AXYL_01677(cphA1) AXYL_01678(cphA2)
AXO: 
AXN: 
CDN: 
RFR: 
POL: 
PNA: 
AAV: 
AJS: 
DIA: 
AAA: 
ACK: 
VEI: 
DAC: 
DEL: 
VAP: 
VPE: 
VPD: 
CTT: 
ADN: 
ADK: 
MPT: 
HAR: 
MMS: 
mma_3555(cphA1) mma_3556(cphA2)
HSE: 
Hsero_2170(cphA2) Hsero_2171(cphA1)
CFU: 
CFU_1324(cphA1) CFU_1325(cphA2)
LCH: 
RGE: 
RGE_40110(cphA) RGE_40120(cphA)
NEU: 
NET: 
NIT: 
NII: 
NMU: 
DAR: 
DSU: 
SDR: 
MEH: 
APP: 
GCA: 
BPRC: 
PPD: 
DTO: 
SCU: 
MCI: 
RLG: 
RLB: 
PAMI: 
OAR: 
SCH: 
ALI: 
BSUB: 
BIF: 
LGY: 
PPOL: 
AAC: 
AAD: 
TC41_0240(cphA)
BTS: 
CPE: 
CPE2213(cphA)
CPF: 
CPF_2477(cphA)
CPR: 
CPR_2187(cphA)
CTC: 
CTET: 
CBO: 
CBO0120(cphA)
CBA: 
CLB_0156(cphA)
CBH: 
CLC_0168(cphA)
CBY: 
CLM_0163(cphA)
CBL: 
CLK_3295(cphA)
CBK: 
CLL_A0470(cphA)
CBB: 
CLD_0666(cphA)
CBI: 
CLJ_B0158(cphA)
CBT: 
CLH_0462(cphA)
CBF: 
CLI_0175(cphA)
CBM: 
CBF_0148(cphA)
CBJ: 
CBE: 
CKL: 
CKL_3725(cphA)
CKR: 
CCE: 
CLJ: 
CLB: 
CSR: 
CSB: 
CAH: 
AMT: 
AOE: 
ASF: 
ASM: 
ASB: 
CTH: 
CTX: 
CCL: 
CAD: 
SWO: 
DSY: 
DHD: 
DDH: 
DDL: 
DKU: 
TJR: 
SGY: 
DOR: 
DAI: 
DMI: 
DED: 
DEC: 
DRS: 
SAY: 
TPY_0184(cphA)
SAP: 
TTE: 
TTE2785(MurE3)
TEX: 
THX: 
TPD: 
TIT: 
TMT: 
TBO: 
TWI: 
TPZ: 
CSC: 
ATE: 
COB: 
CHD: 
COW: 
CKI: 
CKN: 
CLC: 
TOC: 
TTM: 
TTO: 
TXY: 
TSH: 
MAS: 
NTH: 
HHL: 
MMI: 
CCG: 
KSE: 
MPH: 
MLP_14760(cphA)
KFL: 
NML: 
AMS: 
ASE: 
ACPL_6520(cphA)
ACTN: 
L083_6049(cphA)
AFS: 
PLM: 
SACI: 
SYN: 
SYZ: 
SYY: 
SYT: 
SYS: 
SYQ: 
SYR: 
SYX: 
SYP: 
CYB: 
SYNE: 
SYNP: 
TEL: 
THN: 
MAR: 
MAE_27460(cphA)
CYT: 
cce_2237(cphA)
CYP: 
CYC: 
CYN: 
CYH: 
CYJ: 
CAN: 
CSN: 
GLP: 
CMP: 
TER: 
LEP: 
GEI: 
OAC: 
ONI: 
PSEU: 
CEP: 
MIC: 
ARP: 
GVI: 
gvip562(cphA)
GLJ: 
GKIL_2419(cphA)
ANA: 
all3879(cphA) alr0573(cphA)
NPU: 
NOS: 
NOP: 
AVA: 
ANB: 
ACY: 
NAZ: 
CSG: 
CALO: 
CALT: 
RIV: 
CTHE: 
PLP: 
SCS: 
NKO: 
DFE: 
SLI: 
RSI: 
HSW: 
GFO: 
GFO_0745(cphA)
FPS: 
FP1111(cphA)
ASL: 
NDO: 
DDD_3459(cphA)
FBA: 
CCH: 
CPB: 
HAU: 
TRO: 
STI: 
DDR: 
DMR: 
DPD: 
SRB: 
 » show all
Taxonomy
Reference
1  [PMID:11131019]
  Authors
Aboulmagd E, Oppermann-Sanio FB, Steinbuchel A.
  Title
Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308.
  Journal
Arch. Microbiol. 174 (2000) 297-306.
  Organism
Synechocystis sp.
  Sequence
[syn:slr2002]
Reference
2  [PMID:11319097]
  Authors
Aboulmagd E, Oppermann-Sanio FB, Steinbuchel A.
  Title
Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity.
  Journal
Appl. Environ. Microbiol. 67 (2001) 2176-82.
  Organism
Synechocystis sp.
  Sequence
[syn:slr2002]
Reference
3  [PMID:6767688]
  Authors
Allen MM, Hutchison F, Weathers PJ.
  Title
Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308.
  Journal
J. Bacteriol. 141 (1980) 687-93.
Reference
4  [PMID:10951215]
  Authors
Berg H, Ziegler K, Piotukh K, Baier K, Lockau W, Volkmer-Engert R.
  Title
Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers.
  Journal
Eur. J. Biochem. 267 (2000) 5561-70.
  Organism
Synechococcus elongatus
Reference
5  [PMID:12132696]
  Authors
Ziegler K, Deutzmann R, Lockau W.
  Title
Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense.
  Journal
Z. Naturforsch. [C]. 57 (2002) 522-9.
Reference
6  [PMID:9652408]
  Authors
Ziegler K, Diener A, Herpin C, Richter R, Deutzmann R, Lockau W.
  Title
Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin).
  Journal
Eur. J. Biochem. 254 (1998) 154-9.
  Organism
Anabaena variabilis
  Sequence
[ava:Ava_1814]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
131554-17-1

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