KEGG   ENZYME: 6.3.2.43Help
Entry
EC 6.3.2.43                 Enzyme                                 

Name
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase;
alpha-aminoadipate-lysW ligase lysX (gene name);
LysX (ambiguous);
AAA---LysW ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming)
Reaction(IUBMB)
ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate [RN:R09775]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate [CPD:C19885];
L-2-aminoadipate [CPD:C00956]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate [CPD:C19886]
Comment
The enzymes from the thermophilic bacterium Thermus thermophilus and the thermophilic archaea Sulfolobus acidocaldarius and Sulfolobus tokodaii protect the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW. This reaction is part of the lysine biosynthesis pathway in these organisms.
History
EC 6.3.2.43 created 2014
Pathway
Lysine biosynthesis
Metabolic pathways
Orthology
K05827  
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase
Genes
ENF: 
MSD: 
CCX: 
SAUC: 
PMS: 
PMQ: 
PMW: 
PIH: 
CMD: 
SGR: 
SSX: 
SFA: 
STRP: 
F750_6903(lysX)
SCI: 
SGU: 
SPRI: 
SRW: 
TUE45_06896(lysX_3)
SPAV: 
SLC: 
STRT: 
SESP: 
ASE: 
ACPL_7493(rimK)
CAI: 
RRS: 
RCA: 
CAU: 
CAG: 
CHL: 
HAU: 
TRO: 
trd_1519(lysX) trd_A0165(lysX)
STI: 
ATM: 
ANT_08250(lysX) ANT_12410(lysX)
CAP: 
DRA: 
DGE: 
DDR: 
DMR: 
DGO: 
DPD: 
DSW: 
DCH: 
DAB: 
DPU: 
TRA: 
TTH: 
TTJ: 
TTS: 
TTL: 
TSC: 
THC: 
TOS: 
TAQ: 
TBC: 
MRB: 
MRE: 
MSV: 
OPR: 
MHD: 
TTR: 
TAI: 
TLI: 
AMO: 
CPOR: 
MRO: 
CABY: 
HDL: 
HHB: 
Hhub_3648(argX)
HJE: 
HALH: 
HTSR_2057(lysX)
HHSR: 
HSR6_2133(lysX)
HMA: 
rrnAC2679(rimK)
HHI: 
HAH_3083(argX)
HHN: 
HAB: 
NPH: 
NP_5258A(argX)
NMO: 
Nmlp_1243(argX)
HUT: 
HTI: 
HMU: 
HALI: 
HSU: 
HSF: 
HWA: 
HQ_3714A(argX)
HWC: 
Hqrw_4129(argX)
HVO: 
HVO_0046(rimK1)
HME: 
HFX_0047(argX)
HGI: 
HBO: 
HLA: 
HTU: 
HDA: 
NMG: 
HXA: 
NAT: 
NJ7G_0263(argX)
NPE: 
NGE: 
HRU: 
NOU: 
SALI: 
HLR: 
HLC: 
PTO: 
FAC: 
FAI: 
FAD_0508(lysX)
CDIV: 
PHO: 
PH1721(PH1721)
PAB: 
PAB0290(rimK)
PFU: 
PFI: 
PYN: 
PYS: 
TKO: 
THS: 
THV: 
TCH: 
APE: 
APE_1463(lysX)
ACJ: 
ACAM_0923(lysX)
IHO: 
IIS: 
IAG: 
PFM: 
PDL: 
SSO: 
SSO0159(rimK-1)
SOL: 
SSOA: 
SSOL: 
SSOF: 
STO: 
STK_01920(lysX)
SAI: 
SACN: 
SACR: 
SACS: 
SIS: 
SIA: 
SIM: 
SID: 
SIY: 
SIN: 
SII: 
SIH: 
SIR: 
SIC: 
SULA: 
MSE: 
MCN: 
AHO: 
AMAN: 
PAI: 
PIS: 
PCL: 
PAS: 
PYR: 
POG: 
TNE: 
PYW: 
CMA: 
TUZ: 
TTN: 
TTX_0466(lysX_rimK)
VDI: 
VMO: 
NMR: 
NIR: 
NID: 
NIN: 
NKR: 
NCT: 
NMSP_0393(lysX_1) NMSP_0403(lysX_2)
CSY: 
NGA: 
Ngar_c14560(lysX1) Ngar_c29970(lysX2)
NVN: 
NVIE_002660(lysX1) NVIE_016500(lysX2)
NEV: 
TAA: 
NMY3_02221(lysX_1) NMY3_02228(lysX_2)
CSU: 
NBV: 
T478_1043(lysX) T478_1050(lysX)
TAH: 
KCR: 
BARB: 
HAH: 
 » show all
Taxonomy
Reference
1  [PMID:12925802]
  Authors
Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S
  Title
Cloning, expression, purification, crystallization and initial crystallographic analysis of the lysine-biosynthesis LysX protein from Thermus thermophilus HB8.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 59 (2003) 1651-2.
Reference
2  [PMID:19620981]
  Authors
Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M.
  Title
Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.
  Journal
Nat. Chem. Biol. 5 (2009) 673-9.
  Sequence
[ttj:TTHA1907]
Reference
3  [PMID:23434852]
  Authors
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
  Title
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
  Journal
Nat. Chem. Biol. 9 (2013) 277-83.
  Sequence
[sai:Saci_0754]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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