KEGG   ENZYME: 6.3.2.49Help
Entry
EC 6.3.2.49                 Enzyme                                 

Name
L-alanine---L-anticapsin ligase;
BacD;
alanine-anticapsin ligase;
L-Ala-L-anticapsin ligase;
ywfE (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-alanine:L-anticapsin ligase (ADP-forming)
Reaction(IUBMB)
ATP + L-alanine + L-anticapsin = ADP + phosphate + bacilysin [RN:R11064]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-alanine [CPD:C00041];
L-anticapsin [CPD:C20941]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
bacilysin [CPD:C20942]
Comment
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro [1].
History
EC 6.3.2.49 created 2006 as EC 6.3.2.28, trasferred 2015 to EC 6.3.2.49
Pathway
Biosynthesis of antibiotics
Orthology
K13037  
L-alanine-L-anticapsin ligase
Genes
BSU: 
BSU37710(bacD)
BSR: 
I33_3919(bacD)
BSL: 
BSH: 
BSY: 
BSUT: 
BSUL: 
BSUS: 
BSS: 
BST: 
GYO_4157(bacD)
BSO: 
BSN: 
BSQ: 
BSX: 
C663_3676(bacD)
BSP: 
BAY: 
BAQ: 
BYA: 
BAMP: 
BAML: 
BAMA: 
RBAU_3628(bacD)
BAMN: 
BASU_3404(bacD)
BAMB: 
BAMT: 
BAMY: 
BMP: 
NG74_03665(bacD_2)
BAO: 
BAMF_3604(bacD)
BAZ: 
BQL: 
LL3_03915(bacD)
BXH: 
BQY: 
MUS_4149(bacD)
BAMI: 
BAMC: 
BAMF: 
BPU: 
BPUM_3423(bacD)
BPUM: 
BJS: 
BACW: 
BACP: 
BACB: 
BACY: 
BACL: 
BALM: 
SCX: 
 » show all
Taxonomy
Reference
1  [PMID:16030213]
  Authors
Tabata K, Ikeda H, Hashimoto S.
  Title
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
  Journal
J. Bacteriol. 187 (2005) 5195-202.
  Sequence
[bsu:BSU37710]
Reference
2  [PMID:22298000]
  Authors
Tsuda T, Suzuki T, Kojima S
  Title
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 68 (2012) 203-6.
Reference
3  [PMID:22407814]
  Authors
Shomura Y, Hinokuchi E, Ikeda H, Senoo A, Takahashi Y, Saito J, Komori H, Shibata N, Yonetani Y, Higuchi Y
  Title
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.
  Journal
Protein. Sci. 21 (2012) 707-16.
Reference
4  [PMID:23317005]
  Authors
Parker JB, Walsh CT
  Title
Action and timing of BacC and BacD in the late stages of biosynthesis of the dipeptide antibiotic bacilysin.
  Journal
Biochemistry. 52 (2013) 889-901.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system