KEGG   ENZYME: 6.3.2.49Help
Entry
EC 6.3.2.49                 Enzyme                                 

Name
L-alanine---L-anticapsin ligase;
BacD;
alanine-anticapsin ligase;
L-Ala-L-anticapsin ligase;
ywfE (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-alanine:L-anticapsin ligase (ADP-forming)
Reaction(IUBMB)
ATP + L-alanine + L-anticapsin = ADP + phosphate + bacilysin [RN:R11064]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-alanine [CPD:C00041];
L-anticapsin [CPD:C20941]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
bacilysin [CPD:C20942]
Comment
The enzyme, characterized from the bacterium Bacillus subtilis, is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. The enzyme requires Mg2+ or Mn2+ for activity, and has a broad substrate specificity in vitro [1].
History
EC 6.3.2.49 created 2006 as EC 6.3.2.28, trasferred 2015 to EC 6.3.2.49
Pathway
ec01130  Biosynthesis of antibiotics
Orthology
K13037  L-alanine-L-anticapsin ligase
Genes
BUZ: AYM40_00965
BSU: BSU37710(bacD)
BSR: I33_3919(bacD)
BSL: A7A1_0190
BSH: BSU6051_37710(bacD)
BSY: I653_18500
BSUT: BSUB_04007(bacD)
BSUL: BSUA_04007(bacD)
BSUS: Q433_20770
BSS: BSUW23_18625(bacD)
BST: GYO_4157(bacD)
BSO: BSNT_10429(ywfE)
BSQ: B657_37710(bacD)
BSX: C663_3676(bacD)
BAY: RBAM_034900(bacD)
BAQ: BACAU_3519(bacD)
BYA: BANAU_3672(bacD)
BAMP: B938_17910(bacD)
BAML: BAM5036_3420(bacD)
BAMA: RBAU_3628(bacD)
BAMN: BASU_3404(bacD)
BAMB: BAPNAU_3686(bacD)
BAMT: AJ82_19695
BAMY: V529_37620(bacD)
BMP: NG74_03665(bacD_2)
BAO: BAMF_3604(bacD)
BAZ: BAMTA208_19085(bacD)
BQL: LL3_03915(bacD)
BXH: BAXH7_03907(bacD)
BQY: MUS_4149(bacD)
BAMI: KSO_001610
BAMC: U471_36350
BAMF: U722_18645
BPU: BPUM_3423
BPUM: BW16_18175
BPUS: UP12_17620
BJS: MY9_3862
BACW: QR42_17175
BACP: SB24_11245
BACB: OY17_01115
BACY: QF06_17295
BACL: BS34A_40890(bacD)
BALM: BsLM_3801
SLAU: SLA_0354
SALJ: SMD11_5600
 » show all
Taxonomy
Reference
1  [PMID:16030213]
  Authors
Tabata K, Ikeda H, Hashimoto S.
  Title
ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase.
  Journal
J. Bacteriol. 187 (2005) 5195-202.
  Sequence
[bsu:BSU37710]
Reference
2  [PMID:22298000]
  Authors
Tsuda T, Suzuki T, Kojima S
  Title
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an L-amino-acid ligase.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 68 (2012) 203-6.
Reference
3  [PMID:22407814]
  Authors
Shomura Y, Hinokuchi E, Ikeda H, Senoo A, Takahashi Y, Saito J, Komori H, Shibata N, Yonetani Y, Higuchi Y
  Title
Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.
  Journal
Protein. Sci. 21 (2012) 707-16.
Reference
4  [PMID:23317005]
  Authors
Parker JB, Walsh CT
  Title
Action and timing of BacC and BacD in the late stages of biosynthesis of the dipeptide antibiotic bacilysin.
  Journal
Biochemistry. 52 (2013) 889-901.
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.49
IUBMB Enzyme Nomenclature: 6.3.2.49
ExPASy - ENZYME nomenclature database: 6.3.2.49
BRENDA, the Enzyme Database: 6.3.2.49

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