(carboxyethyl)arginine beta-lactam-synthase;
L-2-N-(2-carboxyethyl)arginine cyclo-ligase (AMP-forming)

Ligases;
Forming carbon-nitrogen bonds;
Cyclo-ligases

L-N2-(2-carboxyethyl)arginine cyclo-ligase (AMP-forming)

ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate [RN:R05467]

R05467

ATP [CPD:C00002];
L-N2-(2-carboxyethyl)arginine [CPD:C06655]

Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. It has been proposed [3] that L-N2-(2-carboxyethyl)arginine is first converted into an acyl-AMP by reaction with ATP and loss of diphosphate, and that the beta-lactam ring is then formed by the intramolecular attack of the beta-nitrogen on the activated carboxy group.

1  [PMID:11472170]

Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI.

Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities.

J. Am. Chem. Soc. 123 (2001) 7388-98.

Streptomyces clavuligerus

2  [PMID:12413541]

Townsend CA.

New reactions in clavulanic acid biosynthesis.

Curr. Opin. Chem. Biol. 6 (2002) 583-9.

Streptomyces clavuligerus

3  [PMID:9689037]

Bachmann BO, Li R, Townsend CA.

beta-Lactam synthetase: a new biosynthetic enzyme.

Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 9082-6.

Streptomyces clavuligerus

NCBI-GI: 3420295