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Entry
EC 6.3.4.24                 Enzyme                                 

Name
tyramine---L-glutamate ligase;
mfnD (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
BRITE hierarchy
Sysname
tyramine:L-glutamate gamma-ligase (ADP-forming)
Reaction(IUBMB)
ATP + tyramine + L-glutamate = ADP + phosphate + gamma-glutamyltyramine [RN:R10902]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
tyramine [CPD:C00483];
L-glutamate [CPD:C00025]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
gamma-glutamyltyramine [CPD:C20926]
Comment
The enzyme, which has been characterized from the archaea Methanocaldococcus fervens, participates in the biosynthesis of the cofactor methanofuran. Requires a divalent cation for activity, with Mn2+ giving the highest activity, followed by Mg2+, Co2+, Zn2+, and Fe2+.
History
EC 6.3.4.24 created 2014
Pathway
Methane metabolism
Orthology
K06914  
tyramine---L-glutamate ligase
Genes
HAS: 
MOX: 
MJA: 
MFE: 
MVU: 
MFS: 
MIF: 
MJH: 
MIG: 
MMP: 
MMQ: 
MMX: 
MMZ: 
MMD: 
MMAK: 
MMAO: 
MAE: 
MVN: 
MVO: 
MOK: 
MAC: 
MBA: 
MBY: 
MBW: 
MBAR: 
MMA: 
MMAZ: 
MVC: 
MEK: 
MLS: 
METM: 
MEF: 
MEQ: 
MSJ: 
MSZ: 
MSW: 
MTHE: 
MTHR: 
MHOR: 
MBU: 
MMET: 
MMH: 
MEV: 
MZH: 
MPY: 
MHZ: 
MTP: 
MCJ: 
MHI: 
MHU: 
MLA: 
MEM: 
MBG: 
MEMA: 
MPI: 
MBN: 
MFO: 
MPL: 
MPD: 
MEZ: 
RCI: 
MTH: 
MMG: 
METC: 
MST: 
MSI: 
MRU: 
MEB: 
MMIL: 
MEYE: 
MOL: 
MEL: 
MEW: 
METH: 
MFC: 
MFI: 
MFV: 
MKA: 
AFU: 
AFG: 
AVE: 
AST: 
FPL: 
GAC: 
GAH: 
LOKI: 
BARB: 
 » show all
Taxonomy
Reference
1  [PMID:25211225]
  Authors
Wang Y, Xu H, Harich KC, White RH
  Title
Identification and characterization of a tyramine-glutamate ligase (MfnD) involved in methanofuran biosynthesis.
  Journal
Biochemistry. 53 (2014) 6220-30.
  Sequence
[mja:MJ_0815] [mfe:Mefer_1180]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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