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Entry
EC 6.3.4.24                 Enzyme                                 

Name
tyramine---L-glutamate ligase;
mfnD (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
BRITE hierarchy
Sysname
tyramine:L-glutamate gamma-ligase (ADP-forming)
Reaction(IUBMB)
ATP + tyramine + L-glutamate = ADP + phosphate + gamma-glutamyltyramine [RN:R10902]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
tyramine [CPD:C00483];
L-glutamate [CPD:C00025]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
gamma-glutamyltyramine [CPD:C20926]
Comment
The enzyme, which has been characterized from the archaea Methanocaldococcus fervens, participates in the biosynthesis of the cofactor methanofuran. Requires a divalent cation for activity, with Mn2+ giving the highest activity, followed by Mg2+, Co2+, Zn2+, and Fe2+.
History
EC 6.3.4.24 created 2014
Pathway
ec00680  Methane metabolism
Orthology
K06914  tyramine---L-glutamate ligase
Genes
HAS: Halsa_0958
MOX: DAMO_2657
MJA: MJ_0815
MFE: Mefer_1180
MVU: Metvu_0080
MFS: MFS40622_1347
MIF: Metin_1143
MJH: JH146_1087
MIG: Metig_1750
MMP: MMP0564
MMD: GYY_03655
MAE: Maeo_0448
MVO: Mvol_0760
MAC: MA_1475
MBAR: MSBR2_1211
MBAK: MSBR3_1316
MMA: MM_2510
MMAC: MSMAC_2261
METM: MSMTP_0947
MTHE: MSTHC_0507
MTHR: MSTHT_0225
MHOR: MSHOH_2802
MBU: Mbur_1315
MMET: MCMEM_1762
MMH: Mmah_1619
MPY: Mpsy_2546
MTP: Mthe_1641
MCJ: MCON_1686
MHI: Mhar_1058
MHU: Mhun_0571
MLA: Mlab_0268
MEMA: MMAB1_1685
MPI: Mpet_1053
MBN: Mboo_0920
MPL: Mpal_0581
MPD: MCP_1324
MEZ: Mtc_1029
RCI: RCIX2220
MTH: MTH_835
METC: MTCT_0749
MWO: MWSIV6_1212(mfnD)
METE: tca_00802
MST: Msp_0387
MSI: Msm_0852
MRU: mru_1696
MEB: Abm4_0504
MMIL: sm9_1779
MEYE: TL18_07950
MOL: YLM1_1164
METH: MBMB1_0690
MFC: BRM9_1505
MCUB: MCBB_0701(mfnD)
MFV: Mfer_0210
MKA: MK0114
AFU: AF_1061
FPL: Ferp_2285
GAC: GACE_0439
GAH: GAH_01280
BARB: AOA66_0934(mptN)
LOKI: Lokiarch_26760(ddl)
 » show all
Taxonomy
Reference
1  [PMID:25211225]
  Authors
Wang Y, Xu H, Harich KC, White RH
  Title
Identification and characterization of a tyramine-glutamate ligase (MfnD) involved in methanofuran biosynthesis.
  Journal
Biochemistry. 53 (2014) 6220-30.
  Sequence
[mja:MJ_0815] [mfe:Mefer_1180]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.4.24
IUBMB Enzyme Nomenclature: 6.3.4.24
ExPASy - ENZYME nomenclature database: 6.3.4.24
BRENDA, the Enzyme Database: 6.3.4.24

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